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sdAb anti-ALFA Halo fusion

Immunocytochemistry of PFA-fixed HeLa cells stably expressing a non-fluorescent m-Ruby-ALFA fusion protein. A Greyscale image of the staining performed with sdAb anti-ALFA Halo fusion (Cat. No. N1541, dilution 1:500) and detected with fluorophore-labeled Halo ligand. B Greyscale image of co-staining with the FluoTag®-X2 anti-mRuby coupled to Atto 488 (Cat. No. N3302-At488, dilution 1:500). C The corresponding DAPI signal. D Merge of A-C. E Control staining of HeLa cells using N3302-At488 without N1541.

sdAb anti-ALFA Halo fusion

Cat No: N1541 Category:

275,00 €

The sdAb anti-ALFA Halo fusion combines a single-domain antibody (sdAb) targeting the ALFA tag with a dehalogenase-based technology. The Halo protein tag is fused to the C-terminus of the sdAb anti-ALFA.

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The sdAb anti-ALFA Halo fusion combines an alpaca-derived single-domain antibody (sdAb) targeting the ALFA tag with a dehalogenase-based technology. The Halo protein tag sequence is fused to the C-terminus of the sdAb anti-ALFA. The final application of the sdAb anti-ALFA Halo fusion depends on the Halo ligand used. For instance, when combined with a fluorescent Halo ligand, our sdAb anti-ALFA Halo fusion can be used in immunofluorescence for detailed protein visualization and manipulation.

The ALFA system is NanoTag’s patented technology, consisting of the ALFA tag—a rationally designed epitope tag (SRLEEELRRRLTE) – and a set of high-affinity anti-ALFA sdAbs, available in various formats to suit diverse application needs. To learn more about the ALFA system, please visit our Technology section here.

Halo refers to a modified bacterial enzyme, haloalkane dehalogenase, engineered to covalently bind synthetic ligands. These ligands can be conjugated to various functional groups such as fluorophores, biotin, or beads, enabling numerous biochemical, proteomic, and imaging applications.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
- 50 µg N1541 AB_3668676
Related Products:

FluoTag®-X2 anti-ALFA (Cat. No. N1502)

sdAb anti-ALFA (Cat. No. N1505)

Recombinant anti-ALFA antibody (rHcAb) (Cat. No. N1582 – N1586)
Clone: 1G5
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: For IF (ICC), use the sdAb anti-ALFA Halo fusion at 1:500 dilution.
Capacity: N/A
Antigen: ALFA-tag
Targets: ALFA-tag
Specificity: Recognizes the ALFA tag (SRLEEELRRRLTE).
Formulation:

sdAb anti-ALFA Halo fusion is lyophilized from PBS pH 7.4 with 2% BSA (US-Origin). After reconstitution in 200 μL of sterile 50% glycerol (v/v) in deionized water, the final concentration of sdAb anti-ALFA Halo fusion is 0.25 mg/mL. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 38.0
Ext Coef: 60118
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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