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COS-7 cell transfected with Vimentin-ALFA stained with Recombinant sdAb anti-ALFA Rabbit Fc-fusion (cat. no. N1583) (1:500) and FluoTag-X4 anti-rabbit IgG Atto 488 (cat. no N2404-At488-S). Nuclei were visualized using DAPI.

Recombinant anti-ALFA Antibody (rec. hcAbs)

Cat No: N/A Category:

375,00 €

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The recombinant sdAb anti-ALFA Fc-fusion is a genetic fusion of our established camelid anti-ALFA single-domain antibody to various IgG Fc domains of the most commonly employed host species. This antibody preserves the specificity and high-affinity binding of the anti-ALFA single-domain antibody and adds the features of a conventional IgG type antibody. It can be used in Western blotting and many other applications based on immunodetection (IF, IHC, IP).

MSDS Protocols
Variations:
Amount Conjugation Cat No.
Mouse IgG1 Fc-fusion 100 µg N1582
Rabbit Fc-fusion protein 100 µg N1583
Mouse IgG2 Fc-fusion protein 100 µg N1585
Guinea Pig Fc-fusion 100 µg N1584
Human IgG1 Fc-fusion protein 100 µg N1586
Related Products: -
Clone: 1G5
Host: Alpaca
Produced in: HEK
Application: IF, IHC, WB
Dilution: 1:500 (IF, IHC), 1:1000 WB
Capacity: N/A
Antigen: -
Targets: ALFA-tag
Specificity: Recognizes the ALFA-tag (SRLEEELRRRLTE).
Formulation: Lyophilized from PBS pH 7.4, containing 100 µg rec. hcAbs and 2% BSA (US-Origin). Reconstitute with 100 µL 50% glycerol.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

You can find protocols on our Resources page
References:

Götzke et al. 2019 The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions. Limited Use Label License: Internal Research Use Only The materials you will receive/have received from NanoTag Biotechnologies GmbH (“Materials”) were provided or produced under a service license between NanoTag Biotechnologies GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies”) through the use of Life Technologies proprietary cell lines. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit. Pharmacokinetic and toxicology studies required to meet the regulations for filing an IND (“IND-Enabling Studies”), first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial research (beginning at IND-Enabling Studies and thereafter) or any other commercial purposes or applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008.

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