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COS-7 cell transfected with Vimentin-ALFA stained with Recombinant heavy-chain anti-ALFA antibody (Cat. No. N1583, dilution 1:500) and FluoTag-X4 anti-rabbit IgG Atto 488 (Cat. No N2404-At488-S). Nuclei were visualized using DAPI.

Recombinant anti-ALFA antibody (rHcAb)

Cat No: N/A Category:

400,00 €

The recombinant heavy-chain anti-ALFA antibody is a genetic fusion of our established camelid anti-ALFA single-domain antibody (sdAb) to various IgG Fc domains of the most commonly employed host species.

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The recombinant heavy-chain anti-ALFA antibody is a genetic fusion of our established camelid anti-ALFA single-domain antibody (sdAb) to various IgG Fc domains of the most commonly employed host species. This antibody preserves the specificity and high-affinity binding of the anti-ALFA single-domain antibody and adds the features of a conventional IgG type antibody. It can be used in Western blotting and many other applications based on immunodetection (IF, IHC, IP).

MSDS
Protocols
Reconstitution
Variations:
Conjugation Amount Cat No. RRID
Mouse IgG1 Fc-fusion 100 µg N1582 AB_3065196
Rabbit IgG Fc-fusion 100 µg N1583 AB_3075998
Mouse IgG2 Fc-fusion 100 µg N1585 AB_3076000
Guinea Pig IgG Fc-fusion 100 µg N1584 AB_3075999
Human IgG1 Fc-fusion 100 µg N1586 AB_3076001
Related Products: -
Clone: 1G5
Host: Alpaca
Produced in: HEK
Application: IF, IHC, WB
Dilution: 1:500 (IF, IHC), 1:1000 WB
Capacity: N/A
Antigen: -
Targets: ALFA-tag
Specificity: Recognizes the ALFA-tag (SRLEEELRRRLTE).
Formulation:

Lyophilized from PBS pH 7.4, containing 100 µg rec. hcAbs and 2% BSA (US-Origin). Reconstitute with 100 µL 50% glycerol.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing:

Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

You can find protocols on our Resources page
References:
  1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
  2. Potlapalli BP, Fuchs J, Rutten T, Meister A, Houben A. The potential of ALFA-tag and tyramide-based fluorescence signal amplification to expand the CRISPR-based DNA imaging toolkit. J Exp Bot. Published online August 6, 2024. doi:10.1093/jxb/erae341 (CRISPR-FISH; DNA repeats/centromere labeling, A. thaliana; telomere labeling, N. benthamiana)
  3. Andreu-Carbó M, Egoldt C, Velluz MC, Aumeier C. Microtubule damage shapes the acetylation gradient. Nat Commun. 2024;15(1):2029. Published 2024 Mar 6. doi:10.1038/s41467-024-46379-5 (N1586; IF/ICC; HeLa)
  4. Frodyma DE, Troia TC, Rao C, et al. PGC-1β and ERRα Promote Glutamine Metabolism and Colorectal Cancer Survival via Transcriptional Upregulation of PCK2. Cancers (Basel). 2022;14(19):4879. Published 2022 Oct 5. doi:10.3390/cancers14194879 (WB; human HCT116, HT-29)
  5. Nozawa K, Sogabe T, Hayashi A, et al. In vivo nanoscopic landscape of neurexin ligands underlying anterograde synapse specification. Neuron. 2022;110(19):3168-3185.e8. doi:10.1016/j.neuron.2022.07.027 (IHC; mouse brain)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions. Limited Use Label License: Internal Research Use Only The materials you will receive/have received from NanoTag Biotechnologies GmbH (“Materials”) were provided or produced under a service license between NanoTag Biotechnologies GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies”) through the use of Life Technologies proprietary cell lines. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit. Pharmacokinetic and toxicology studies required to meet the regulations for filing an IND (“IND-Enabling Studies”), first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial research (beginning at IND-Enabling Studies and thereafter) or any other commercial purposes or applications of any kind, including, without limitation, quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008.

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