Formulation:
|
DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi,150 mM NaCl, and 1 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL 50% glycerol for storage at -80 °C. The N1505-HRP is lyophilized from 200 µL HRP protector buffer and should be reconstituted with 200 µL pure deionized water. and Store at 4°C.
|
References:
|
- Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
- Mougios N, Cotroneo ER, Imse N, et al. NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals. Nat Commun. 2024;15(1):8771. Published 2024 Oct 10. doi:10.1038/s41467-024-53030-w
- Shaib AH, Chouaib AA, Chowdhury R, et al. One-step nanoscale expansion microscopy reveals individual protein shapes. Nat Biotechnol. Published online October 9, 2024. doi:10.1038/s41587-024-02431-9
- Hellmeier J, Strauss S, Xu S, et al. Quantification of absolute labeling efficiency at the single-protein level. Nat Methods. Published online April 24, 2024. doi:10.1038/s41592-024-02242-5 (ICC/IF; DNA-PAINT)
- van Zwam MC, Dhar A, Bosman W, et al. IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms. PLoS Biol. 2024;22(3):e3002551. Published 2024 Mar 11. doi:10.1371/journal.pbio.3002551 (N1505-HRP; WB; HT1080 cells)
- Zhang L, Stauffer WT, Wang JS, et al. Recruitment of Polo-like kinase couples synapsis to meiotic progression via inactivation of CHK-2. Elife. 2023;12:e84492. Published 2023 Jan 26. doi:10.7554/eLife.84492 (WB; C. elegans)
- Igreja C, Loschko T, Schäfer A, et al. Application of ALFA-Tagging in the Nematode Model Organisms Caenorhabditis elegans and Pristionchus pacificus. Cells. 2022;11(23):3875. Published 2022 Dec 1. doi:10.3390/cells11233875 (WB; C. elegans, P. pacificus)
- Akhuli D, Dhar A, Viji AS, Bhojappa B, Palani S. ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in Yeast. mSphere. 2022;7(5):e0033322. doi:10.1128/msphere.00333-22 (WB; yeast)
- Buglino JA, Ozakman Y, Xu Y, Chowdhury F, Tan DS, Glickman MS. Diisonitrile Lipopeptides Mediate Resistance to Copper Starvation in Pathogenic Mycobacteria. mBio. 2022;13(5):e0251322. doi:10.1128/mbio.02513-22 (WB; M. tuberculosis)
- Carsten A, Rudolph M, Weihs T, et al. MINFLUX imaging of a bacterial molecular machine at nanometer resolution. Methods Appl Fluoresc. 2022;11(1):10.1088/2050-6120/aca880. Published 2022 Dec 13. doi:10.1088/2050-6120/aca880 (WB; HeLa infected with Y. enterocolitica)
- Kusakari K, Machida T, Ishida Y, et al. The complex formation of MASP-3 with pattern recognition molecules of the lectin complement pathway retains MASP-3 in the circulation. Front Immunol. 2022;13:907023. Published 2022 Aug 16. doi:10.3389/fimmu.2022.907023 (Homodimer formation)
- Xu J, Kim AR, Cheloha RW, et al. Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies. Elife. 2022;11:e74326. Published 2022 Jan 25. doi:10.7554/eLife.74326 (WB; fruit fly)
- Zhang Q, Miyamoto A, Watanabe S, et al. Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods. 2022;2(10):100301. Published 2022 Sep 20. doi:10.1016/j.crmeth.2022.100301 (IF, multiplexed super-resolution microscopy; Xenopus laevis XTC cells)
- Gomkale R, Linden A, Neumann P, et al. Mapping protein interactions in the active TOM-TIM23 supercomplex. Nat Commun. 2021;12(1):5715. Published 2021 Sep 29. doi:10.1038/s41467-021-26016-1 (WB; yeast)
|