275,00 € – 625,00 €
In-house developed single-domain antibody (sdAb) that recognizes a small rationally-designed epitope tag ALFA.
The ALFA tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) and is part of the proprietary technology of NanoTag. In contrast to most conventional epitope tags, the ALFA tag was developed based on rational considerations, which enabled us to equip it with desired features (learn more about the ALFA system here). The ALFA tag, in combination with three sdAbs, offers a unique platform to employ a single epitope tag in virtually all biochemical and biomedical applications. A full characterization of the system can be found in an open source article published in Nat. Commun. in 2019.
Variations: |
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Related Products: |
FluoTag®-X2 anti-ALFA (Cat. No. N1502) sdAb anti-ALFA Halo fusion (Cat. No. 1541) Recombinant anti-ALFA antibody (rHcAb) (Cat. No. N1582 – N1586) |
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Clone: | 1G5 | ||||||||||||||||||||
Host: | Alpaca | ||||||||||||||||||||
Produced in: | E.coli | ||||||||||||||||||||
Application: | IF, WB | ||||||||||||||||||||
Dilution: | ELISA with sdAb anti-ALFA HRP 1:5000 | ||||||||||||||||||||
Capacity: | N/A | ||||||||||||||||||||
Antigen: | - | ||||||||||||||||||||
Targets: | ALFA-tag | ||||||||||||||||||||
Specificity: | Recognizes the ALFA tag (SRLEEELRRRLTE). | ||||||||||||||||||||
Formulation: |
DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. The HRP-conjugated sdAbs are lyophilized from HRP protector buffer and should be reconstituted with 200 µL pure deionized water for storage at 4°C or with 200 µL high purity, sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage. |
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kDa: | 16.0 | ||||||||||||||||||||
Ext Coef: | 18638 | ||||||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
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Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page. |
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References: |
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Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |