The ALFA tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) and is part of the proprietary technology of NanoTag. In contrast to most conventional epitope tags, the ALFA tag was developed based on rational considerations, which enabled us to equip it with desired features (learn more about the ALFA system here). The ALFA tag, in combination with three sdAbs, offers a unique platform to employ a single epitope tag in virtually all biochemical and biomedical applications. A full characterization of the system can be found in an open source article published in Nat. Commun. in 2019.
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MSDS datasheets for HRP conjugated sdAbs can be found here |
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Clone: | 1G5 | |||||||||||||||
Host: | Alpaca | |||||||||||||||
Produced in: | E.coli | |||||||||||||||
Application: | IF, WB | |||||||||||||||
Dilution: | ELISA with sdAb anti-ALFA HRP 1:5000 | |||||||||||||||
Capacity: | N/A | |||||||||||||||
Antigen: | - | |||||||||||||||
Targets: | ALFA-tag | |||||||||||||||
Specificity: | Recognizes the ALFA tag (SRLEEELRRRLTE). | |||||||||||||||
Formulation: | DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi,150 mM NaCl, and 1 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL 50% glycerol for storage at -80 °C. The N1505-HRP is lyophilized from 200 µL HRP protector buffer and should be reconstituted with 200 µL pure deionized water. and Store at 4°C. | |||||||||||||||
kDa: | 16.0 | |||||||||||||||
Ext Coef: | 18638 | |||||||||||||||
Shipping: | Ambient temperature | |||||||||||||||
Storing: | Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below. | |||||||||||||||
Protocols: | - | |||||||||||||||
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Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | |||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |