Immunostaining of HeLa cells expressing an ALFA-Vimentin fusion protein with an anti-ALFA sdAb (Cat. No. N1505-DBCO, dilution 1:500) bearing a 10-nucleotide single-stranded DNA, imaged by 3D DNA-PAINT. The histogram refers to the region marked by a small yellow rectangle where 2 individual vimentin filaments are resolved although being only ~90 nm apart. The localization precision was 5.2 nm. (Figure modified from Götzke et al. Nat Commun (2019).)
250,00 € – 600,00 €
The ALFA tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) and is part of the proprietary technology of NanoTag. In contrast to most conventional epitope tags, the ALFA tag was developed based on rational considerations, which enabled us to equip it with desired features (learn more about the ALFA system here). The ALFA tag, in combination with three sdAbs, offers a unique platform to employ a single epitope tag in virtually all biochemical and biomedical applications. A full characterization of the system can be found in an open source article published in Nat. Commun. in 2019.
|Specificity:||Recognizes the ALFA tag (SRLEEELRRRLTE).|
|Formulation:||DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi,150 mM NaCl, and 1 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL 50% glycerol for storage at -80 °C. The N1505-HRP is lyophilized from 200 µL HRP protector buffer and should be reconstituted with 200 µL pure deionized water. and Store at 4°C.|
|Storing:||Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.|
Götzke et al. 2019 The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
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