Single-domain antibody discovery services tailored to your needs

Our experience combined with proprietary technologies provides an excellent choice for your nanobody discovery projects. The NanoTag discovery workflow is highly customizable to the needs of your project, ranging from complex counter selections steps, promoting conformation-specific epitopes, special salt and pH working conditions for binding, and other screening variations that have been carried out successfully in our facility.

Highly experienced scientists, dedicated laboratories, and our own animal research farm will facilitate your challenging project

With a highly customizable service, we can assist at every level of a nanobody discovery project, from antigen design and production, tailored screenings, to candidate validation and scale-up production.

Discovery projects can be stopped at different exit points if requested following reports at defined milestones. For example, if the immune response of the immunized animals is not strong enough after the immunization phase, there will be the option of stopping or prolonging the immunization.

NanoTag has all the necessary permission from the authorities to perform immunizations only minutes away from our main laboratories. This ensures the best blood quality possible and RNA preservation, which results in an exceptional diversity of nanobodies needed for a thorough and deep screening.

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Our fully customizable service allows clients to decide at which stage in the discovery process the material should be transferred (blood, cDNA, library, or single clones delivery)
1-4 weeks
The client provides the antigens needed for the immunization of animals and for the screening phase.
Alternatively, NanoTag can produce antigens for you as an optional R&D service.
6-12 weeks
Our experienced scientists will coordinate with you the best-suited immunization scheme to perform in our research farm.
1-2 weeks
Library generation:
After the final boost, 150 ml of blood is drawn from each animal, and PBMCs are separated to finally make an mRNA extraction and produce a cDNA library using a special set of primers that results in an enhanced VHH diversity.
4-6 weeks
Phage display screening:
We have optimized several steps from the conventional phage display procedures. Starting with the generation of an enhanced VHH library diversity at the cDNA level due to our primers, but also, we have optimized the transformation into bacteria to minimize this bottleneck in diversity.
Additionally, we have developed a series of antigen immobilization methodologies that allow very clean panning rounds, maximizing the retention of relevant clones.
Positive clones first validation in vitro:
After obtaining the sequences of positively selected clones and grouped in families, non-redundant clones are produced & purified in a mid-scale for more thorough in vitro testing.
Basic validation will be performed in ELISA, using ramp concentrations of antigen to obtain a relative affinity between candidates. More thorough and custom validations are at this stage also possible (e.g. affinity measurements, client-specific testing, etc.)