sdAb anti-RFP

Cat No: N0405 Category:

275,00 625,00 

In-house developed single-domain antibody (sdAb) that binds specifically and strongly to DsRed, mRFP, mCherry, mScalet and others DsRed family members.

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One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives.

Our sdAb anti-RFP clone 2B12 binds specifically and strongly to DsRed, mRFP, mCherry, mScalet and others DsRed family members. Have a look at our FP Specificity Chart.

Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N0405-250ug AB_3075924
Biotin 250 μg N0405-Biotin AB_3075925
DBCO 250 μg N0405-DBCO AB_3075926
Related Products:

FluoTag®-X2 anti-mScarlet-i (Cat. No. N1302)

FluoTag®-X4 anti-RFP (Cat. No. N0404)

Clone: 2B12
Host: Alpaca
Produced in: E.coli
Application:

IF

Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly.

Dilution: N/A
Capacity: N/A
Antigen: mCherry
Targets: mRFP, mScarlet-i, mCherry
Specificity: mRFP (red fluorescent protein) and other derivatives like mOrange, DsRed1, DsRED2, tdTomato, mCherry and mScarlet-i.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.

kDa: 16.2
Ext Coef: 32618
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.

Protocols:

For more details, click the Protocols button above or check our FP Specificity Chart.

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.