275,00 € – 625,00 €
In-house developed single-domain antibody (sdAb) that binds specifically and strongly to DsRed, mRFP, mCherry, mScalet and others DsRed family members.
One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives.
Our sdAb anti-RFP clone 2B12 binds specifically and strongly to DsRed, mRFP, mCherry, mScalet and others DsRed family members. Have a look at our FP Specificity Chart.
Variations: |
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Clone: | 2B12 | ||||||||||||||||
Host: | Alpaca | ||||||||||||||||
Produced in: | E.coli | ||||||||||||||||
Application: |
IF Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly. |
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Dilution: | N/A | ||||||||||||||||
Capacity: | N/A | ||||||||||||||||
Antigen: | mCherry | ||||||||||||||||
Targets: | mRFP, mScarlet-i, mCherry | ||||||||||||||||
Specificity: | mRFP (red fluorescent protein) and other derivatives like mOrange, DsRed1, DsRED2, tdTomato, mCherry and mScarlet-i. | ||||||||||||||||
Formulation: |
DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage. |
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kDa: | 16.2 | ||||||||||||||||
Ext Coef: | 32618 | ||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
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Protocols: |
For more details, click the Protocols button above or check our FP Specificity Chart. |
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References: | - | ||||||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |