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Immunostaining of PFA fixed 3T3 cells expressing a TOM70-mCherry reporter protein with FluoTag®-X4 Atto 647N anti-RFP sdAb (Cat. No. N0404, dilution 1:500, the mCherry signal is represented in red, the corresponding FluoTag-signal is represented in green and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue).

sdAb anti-RFP

Cat No: N0405 Category:

275,00 € – 625,00 €

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One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives.

Our nanobodies bind specifically and strongly to DsRed, RFP, and mCherry, among others family members. Look at our specificity chart in the resource section.

 

MSDS
Protocols
Reconstitution
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N0405-250ug AB_3075924
Biotin 250 μg N0405-Biotin AB_3075925
DBCO 250 μg N0405-DBCO AB_3075926
Related Products: -
Clone: 2B12
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: mRFP
Specificity: mRFP (red fluorescent protein) and other derivatives like mOrange, dsRed1, dsRED2, tdTomato, mCherry and mScarlet-i.
Formulation: DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyohilized from 10 mM KPi,150 mM NaCl, 1 mM EDTA pH 6.0. Reconsitute with 250 µL pure water for immidate use or with 250 µL 50% glycerol for storage at -80 °C.
kDa: 16.2
Ext Coef: 32618
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for upto 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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