Immunostaining of PFA fixed brain sections expressing an ALFA-tag fusion protein with FluoTag®-X2 Sulfo Cyanin 3 anti-ALFA sdAb (Cat. No. N1502, dilution 1:1000; red). Nuclei were visualized with DAPI (blue).

Immunostaining of PFA fixed Cos7 cells expressing either an ALFA-Vimentin fusion protein (left) or a TOM70-GFP-ALFA fusion protein (right) with FluoTag®-X2 anti-ALFA Abberior STAR 635P (Cat. No. N1502-Ab635P-L, dilution 1:500, red). Nuclei were visualized by DAPI staining (blue).

dSTORM image with corresponding bright-field insert of the engineered FtsZ-ALFA E.coli strain, where the ALFA-tag was inserted into the chromosomal copy of the ftsZ gene [Westlund et al., 2023, Curr Genet]. The FtsZ-ALFA was detected with the FluoTag®-X2 anti-ALFA coupled to Janelia Fluor® 635b (Cat. No. N1502-JF635B-L). The Full Width at Half Maximum (FWHM) of FtsZ rings was extracted from line scans as indicated by the green dotted lines in the upper and lower E. coli cells. Image courtesy of Bill Söderström, Australian Institute for Microbiology and Infection, University of Technology Sydney, Australia.

Overlay of bright-field and super-resolution STED images of FtsZ-ALFA expressed in E.coli using plasmids and detected with FluoTag®-X2 anti-ALFA coupled to Atto 488 (Cat. No. N1502-At488-L, dilution 1:500). Image courtesy of Bill Söderström, Australian Institute for Microbiology and Infection, University of Technology Sydney, Australia.

FluoTag®-X2 anti-ALFA

Name: FluoTag®-X2 anti-ALFA
SKU: N1502
Price: 400.00 EUR
Availability: InStock

Cat No: N1502 Category: Primary sdAbs

400,00 €

FluoTag®-X2 anti-ALFA is a fluorescently labeled single-domain antibody (sdAb) that specifically detects the small, rationally designed ALFA epitope tag. Enables direct and highly specific fluorescent detection of ALFA-tagged targets without the need for secondary antibodies.

Clear

FluoTag®-X2 anti-ALFA is derived from an in-house developed single-domain antibody (sdAb) that recognizes a small rationally-designed epitope tag: the ALFA-tag. A full characterization of the system can be found in an open source article published in Nat. Commun. in 2019.

Our FluoTag®-X2 series binds in a monovalent fashion with high affinity and specificity irrespective of whether the sample is alive or fixed with 4% paraformaldehyde, 2% glutaraldehyde, or methanol. Additionally, like all X2 FluoTags, it features two site-specifically coupled fluorophores per sdAb.

Owing to the small size of our FluoTags® and the short ALFA-tag (15 amino acids; PSRLEEELRRRLTEP), the distance between the target epitope and each fluorophore is below 3 nm. Proteins of interest can be tagged with our ALFA-tag at the N-termini, C-termini, or in-between domains and they can also be immunoprecipitated or natively purified with complexes or partner binders using our series of ALFA-Selector resins with physiological elution capability.

Therefore, this ALFA-tag and the companion FluoTags®-X2 anti-ALFA and ALFA-Selectors are the perfect choices for fluorescent-based experiments that want to be combined with biochemical purifications, mass-spectrometry, or any other high-end application.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS

Variations:

Conjugation	Amount	Cat No.	RRID
Janelia Fluor 635b	200 μl	N1502-JF635B-L	AB_3668670
Atto488	200 μl	N1502-At488-L	AB_3075982
AZDye568	200 μl	N1502-AF568-L	AB_3075980
Atto643	200 μl	N1502-At643-L	AB_3075983
Alexa647	200 μl	N1502-AF647-L	AB_3075981
LI-COR IRDye 680RD	200 μl	N1502-Li680-L	AB_3075984
LI-COR IRDye 800CW	200 μl	N1502-Li800-L	AB_3075985
AbberiorStar635P	200 μl	N1502-Ab635P-L	AB_3075979

FluoTag®-X2 anti-ALFA

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