Immunostaining of PFA fixed brain sections expressing an ALFA tag fusion protein with FluoTag®-X2 Sulfo Cyanin 3 anti-ALFA sdAb (Cat. No. N1502, dilution 1:1000; the FluoTag®-signal is represented in red). Nuclei were visualized by DAPI staining (blue).

Immunostaining of PFA fixed Cos7 cells expressing either a TOM70-GFP-ALFA fusion protein (right) or an ALFA-Vimentin fusion protein (left) with FluoTag®-X2 Abberior STAR 635P anti-ALFA sdAb (Cat. No. N1502, dilution 1:500, the FluoTag®-signal is represented in red). Nuclei were visualized by DAPI staining (blue).

Overlay of bright-field and super-resolution STED images of FtsZ-ALFA expressed in E.coli using plasmids and detected with FluoTag®-X2 anti-ALFA coupled to Atto 488 (Cat. No. N1502-At488-L, dilution 1:500). Image courtesy of Bill Söderström, Australian Institute for Microbiology and Infection, University of Technology Sydney, Australia.

FluoTag®-X2 anti-ALFA

Name: FluoTag®-X2 anti-ALFA
SKU: N1502
Price: 400.00 EUR
Availability: InStock

Cat No: N1502 Category: Single-domain antibodies

400,00 €

FluoTag®-X2 anti-ALFA is derived from an in-house developed single-domain antibody (sdAb) that recognizes a small rationally-designed epitope tag: the ALFA tag.

Clear

FluoTag®-X2 anti-ALFA is derived from an in-house developed single-domain antibody (sdAb) that recognizes a small rationally-designed epitope tag: the ALFA tag. A full characterization of the system can be found in an open source article published in Nat. Commun. in 2019.

Our FluoTag-X2 series binds in a monovalent fashion with high affinity and specificity irrespective of whether the sample is alive or fixed with 4% paraformaldehyde, 2% glutaraldehyde, or methanol. Additionally, like all X2 FluoTags, it features two site-specifically coupled fluorophores per sdAb.

Owing to the small size of our FluoTags and the short ALFA-tag (15 amino acids; PSRLEEELRRRLTEP), the distance between the target epitope and each fluorophore is below 3 nm. Proteins of interest can be tagged with our ALFA-tag at the N-termini, C-termini, or in-between domains and they can also be immunoprecipitated or natively purified with complexes or partner binders using our series of ALFA-Selector resins with physiological elution capability.

Therefore, this ALFA tag and the companion FluoTags®-X2 anti-ALFA and ALFA-Selectors are the perfect choices for fluorescent-based experiments that want to be combined with biochemical purifications, mass-spectrometry, or any other high-end application.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS Protocols

Variations:

Conjugation	Amount	Cat No.	RRID
Atto488	200 μl	N1502-At488-L	AB_3075982
AZDye568	200 μl	N1502-AF568-L	AB_3075980
Atto643	200 μl	N1502-At643-L	AB_3075983
Alexa647	200 μl	N1502-AF647-L	AB_3075981
LI-COR IRDye 680RD	200 μl	N1502-Li680-L	AB_3075984
LI-COR IRDye 800CW	200 μl	N1502-Li800-L	AB_3075985
AbberiorStar635P	200 μl	N1502-Ab635P-L	AB_3075979

FluoTag®-X2 anti-ALFA

Related products

ALFA Selector CE (Incl. 10 mg elution peptide) – 4% cross-linked agarose, 2000 μl

ALFA Selector ST – 4% cross-linked agarose, 2000 μl

sdAb anti-ALFA