Immunostaining of PFA fixed rat hippocampus neurons with FluoTag-X2 anti-Synaptotagmin-1 Atto 488 (Cat. No. N2302-At488-L, dilution 1:500; green) and anti-Gephyrin Cy3 (Synaptic Systems Cat. No. 147 011C3). Nuclei have been visualized by DAPI staining (blue).
PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to Alexa Fluor 647 (Cat. No. N2302-AF647, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-AF647. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-At488. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in magenta, B in green and C in blue.
PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to AZDye 568 (Cat. No. N2302-AF568, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-AF568. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-AF647. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in red, B in magenta and C in blue.
PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) coupled to Atto 488 (Cat. No. N2302-At488, dilution 1:500). A Greyscale image of the staining performed with the anti-SYT1 single-domain antibody N2302-At488. B Greyscale image of the counterstaining performed with an anti-GFAP single-domain antibody N3802-AF647. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in green, B in magenta and C in blue.
FluoTag®-X2 anti-Synaptotagmin 1 (7D5)
400,00 €
FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) is an alpaca single-domain antibody (sdAb) that recognizes specifically the cytoplasmic domain of murine Synaptotagmin 1 with high affinity and specificity.
Synaptotagmin 1 (Syt1) belongs to a family with 17 members. Synaptotagmins are all single-pass transmembrane proteins containing two domains binding to calcium at their intracellular portion. Synaptotagmin 1 is enriched at the pre-synaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase of calcium concentration.
Our nanobody recognizes strongly and specifically the cytosolic portion of SYT1.
FluoTags are directly conjugated to fluorophores, however, they can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores, decorating the target protein with 4 fluorophores in total (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.
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| Clone: | 7D5 | ||||||||||||||||||||||||
| Host: | Alpaca | ||||||||||||||||||||||||
| Produced in: | E.coli | ||||||||||||||||||||||||
| Application: | IF | ||||||||||||||||||||||||
| Dilution: | 1:500 (corresponding to 5 nM final concentration) | ||||||||||||||||||||||||
| Capacity: | N/A | ||||||||||||||||||||||||
| Antigen: | - | ||||||||||||||||||||||||
| Targets: | Synaptotagmin 1 (cytosolic) | ||||||||||||||||||||||||
| Specificity: | Recognizes the cytosolic domain of rat and mouse Synaptotagmin 1 (other species not tested). | ||||||||||||||||||||||||
| Formulation: | The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM | ||||||||||||||||||||||||
| kDa: | - | ||||||||||||||||||||||||
| Ext Coef: | - | ||||||||||||||||||||||||
| Shipping: | Ambient temperature | ||||||||||||||||||||||||
| Storing: | Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C. | ||||||||||||||||||||||||
| Protocols: |
This Product is not recommended to be used to detect proteins in Western Blott, sdAbs tend to recognize native/folded protein mainly. Look at detailed protocols in our Resource Section. |
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| Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||||||||
| Legal terms: | By purchasing this product you agree to our general terms and conditions. |
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