400,00 €
FluoTag®-X2 anti-TagFP is an in-house developed single-domain antibody (sdAb) with high affinity and specificity for fluorescent proteins derived from Entacmaea quadricolor, including TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate, and mKate2. It features two fluorophores per sdAb molecule (X2).
Fluorescent proteins (FPs), widely used as protein tags in cell biology and microscopy, have revolutionized protein imaging and biochemical applications. TagRFP, a monomeric red fluorescent protein, was engineered from TurboRFP (derived from Entacmaea quadricolor) to exhibit an extended fluorescence lifetime and enhanced photostability (Merzlyak et al., 2007). Through further mutagenesis, this red-emitting protein was transformed into the blue-emitting TagBFP (Subach et al., 2008). A few years later, an improved version known as mTagBFP2 was introduced, offering greater brightness and stability (Subach et al., 2011). In 2007, mKate was developed from TagRFP, noted for its excellent far-red fluorescence and photostability (Shcherbo et al., 2007). The most currently available variants can be found on an open-source Fluorescent Protein Database FPbase.
FluoTag®-X2 anti-TagFP binds strongly to TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate and mKate2. For detailed information, please refer to our FP Specificity Chart.
FluoTags® are single-domain antibodies (sdAbs) directly conjugated to a defined number of fluorophores. FluoTag®-Q is sdAb equipped with a single fluorophore for more quantitative readouts. FluoTag®-X2 carries two fluorophores per sdAb. FluoTag®-X4 is a blend of two different sdAbs that bind simultaneously to the single target, each bearing two fluorophores, thereby decorating the target protein with 4 fluorophores in total for a brighter signal. For more detailed information on FluoTags®, please check our Technology Section.
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Related Products: | - | ||||||||||||||||||||||||
Clone: | 1H7 | ||||||||||||||||||||||||
Host: | Alpaca | ||||||||||||||||||||||||
Produced in: | E.coli | ||||||||||||||||||||||||
Application: |
IF (ICC) Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly. |
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Dilution: | 1:500 (corresponding to 5 nM final concentration) | ||||||||||||||||||||||||
Capacity: | N/A | ||||||||||||||||||||||||
Antigen: | - | ||||||||||||||||||||||||
Targets: | TagFP | ||||||||||||||||||||||||
Specificity: | Recognizes TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate and mKate2. Likely reactive with other untested TagRFP derivatives. | ||||||||||||||||||||||||
Formulation: |
The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). For more details, click the Protocols button above and check Reconstitution and Storage. |
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kDa: | - | ||||||||||||||||||||||||
Ext Coef: | - | ||||||||||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
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Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and review the FP Specificity Chart. |
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Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||||||||
Legal terms: | By purchasing this product, you agree to our general Terms & Conditions. |