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Immunostaining of PFA fixed 3T3 cells expressing a TOM70-BFP reporter protein with FluoTag®-X2 Abberior STAR 580 anti-TagFP sdAb (Cat. No. N0502, dilution 1:500, the BFP signal is represented in blue, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in magenta).

FluoTag®-X2 anti-TagFP

Cat No: N0502 Category:

375,00 €

FluoTag®-X2 anti-TagFP is derived from an in-house developed single-domain antibody (sdAb) that recognizes fluorescent proteins deriving from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657 and mKate with high affinity and specificity.

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TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2. (change!!!!)

Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2. Look at our specificity chart in the resource section.

For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.

MSDS
Variations:
Conjugation Amount Cat No.
AbberiorStar580 200 μl N0502-Ab580-L
AbberiorStar635P 200 μl N0502-Ab635P-L
Alexa647 200 μl N0502-AF647-L
Atto488 200 μl N0502-At488-L
AZDye568 200 μl N0502-AF568-L
Related Products: -
Clone: 1H7
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500
Capacity: N/A
Antigen: tagBFP
Specificity: Recognizes TagRFP, Tag BFP and mTagBFP2.
Formulation: Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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