Immunostaining of PFA fixed 3T3 cells expressing a TOM70-BFP reporter protein with FluoTag®-X2 Abberior STAR 580 anti-TagFP sdAb (Cat. No. N0502, dilution 1:500, the BFP signal is represented in blue, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in magenta).
FluoTag®-X2 anti-TagFP is derived from an in-house developed single-domain antibody (sdAb) that recognizes fluorescent proteins deriving from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657 and mKate with high affinity and specificity.
TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2. (change!!!!)
Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2. Look at our specificity chart in the resource section.
For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.
|Specificity:||Recognizes TagRFP, Tag BFP and mTagBFP2.|
|Formulation:||Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
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