Immunostaining of PFA fixed 3T3 cells expressing a TOM70-BFP reporter protein with FluoTag®-X2 Abberior STAR 580 anti-TagFP sdAb (Cat. No. N0502, dilution 1:500). The BFP signal is represented in blue, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in magenta.

PFA-fixed Cos7 cells expressing a TOM70-BFP fusion protein were stained with FluoTag®-X2 anti-TagFP coupled to Alexa Fluor 647 (Cat. No. N0502-AF647, dilution 1:500). A Greyscale image of the staining performed with N0502-AF647. B False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-BFP fusion protein were stained with FluoTag®-X2 anti-TagFP coupled to AZDye 568 (Cat. No. N0502-AF568, dilution 1:500). A Greyscale image of the staining performed with N0502-AF568. B False color representation of the image shown in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-BFP fusion protein were stained with FluoTag®-X2 anti-TagFP coupled to Atto 488 (Cat. No. N0502-At488, dilution 1:500). A Greyscale image of the staining performed with N0502-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.

FluoTag®-X2 anti-TagFP

Name: FluoTag®-X2 anti-TagFP
SKU: N0502
Price: 400.00 EUR
Availability: InStock

Cat No: N0502 Category: Primary sdAbs

400,00 €

FluoTag®-X2 anti-TagFP is an in-house developed single-domain antibody (sdAb) with high affinity and specificity for fluorescent proteins derived from Entacmaea quadricolor, including TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate, and mKate2. It features two fluorophores per sdAb molecule (X2).

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Fluorescent proteins (FPs), widely used as protein tags in cell biology and microscopy, have revolutionized protein imaging and biochemical applications. TagRFP, a monomeric red fluorescent protein, was engineered from TurboRFP (derived from Entacmaea quadricolor) to exhibit an extended fluorescence lifetime and enhanced photostability (Merzlyak et al., 2007). Through further mutagenesis, this red-emitting protein was transformed into the blue-emitting TagBFP (Subach et al., 2008). A few years later, an improved version known as mTagBFP2 was introduced, offering greater brightness and stability (Subach et al., 2011). In 2007, mKate was developed from TagRFP, noted for its excellent far-red fluorescence and photostability (Shcherbo et al., 2007). The most currently available variants can be found on an open-source Fluorescent Protein Database FPbase.

FluoTag®-X2 anti-TagFP binds strongly to TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate and mKate2. For detailed information, please refer to our FP Specificity Chart.

FluoTags® are single-domain antibodies (sdAbs) directly conjugated to a defined number of fluorophores. FluoTag®-Q is sdAb equipped with a single fluorophore for more quantitative readouts. FluoTag®-X2 carries two fluorophores per sdAb. FluoTag®-X4 is a blend of two different sdAbs that bind simultaneously to the single target, each bearing two fluorophores, thereby decorating the target protein with 4 fluorophores in total for a brighter signal. For more detailed information on FluoTags®, please check our Technology Section.

MSDS

Variations:

Conjugation	Amount	Cat No.	RRID
Atto488	200 μl	N0502-At488-L	AB_2744623
AZDye568	200 μl	N0502-AF568-L	AB_3075935
Atto643	200 μl	N0502-At643-L	AB_3075938
Alexa647	200 μl	N0502-AF647-L	AB_3075936
AbberiorStar635P	200 μl	N0502-Ab635P-L	AB_2744622

FluoTag®-X2 anti-TagFP

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