A serendipitous discovery in the early 90s at the Free University of Brussels resulted in Dr. Hamers-Casterman finding a new class of antibodies lacking the light chains in the blood of camels (1). These heavy-chain-only antibodies (HcAbs) can be found in camels, dromedaries, and all the new world camels (llamas, alpacas, guanacos & vicuñas).
The minimal domain necessary to bind an epitope/antigen is what is today known as a single-domain antibody (sdAb), variable domain of the heavy-chain-only antibody (VHH), and commercially as Nanobody® (a trademark of Ablynx, now Sanofi).
NanoTag Biotechnologies GmbH has extensive experience and proprietary technologies to screen, select, engineer, validate, and produce specific nanobodies for various applications.
SdAbs keep gaining popularity in multiple applications, from super-resolution microscopy (2–6), affinity purifications (7–10), crystallography (9,11–13), biosensors (14–16), cryo-EM (8,17,18) to medical diagnostics (19,20), clinical imaging (21,22), theragnostic (23), monoclonal and cell therapies (24).
This broad spectrum of applications is due to several unique features that make them attractive when compared to conventional antibodies.
At NanoTag Biotechnologies, we offer tailored sdAb discovery services for your protein of interest, custom sdAb conjugation, and a validated off-the-shelf product portfolio. The sdAbs that are directly coupled to fluorophores can be found as FluoTags®, or if immobilized on agarose and magnetic agarose beads, they can be found as Selectors.
In addition, our sdAbs are also available in an unconjugated version or as sdAbs bearing biotin, cup-per-free click chemistry DBCO, enzymes like the Horse Radish Peroxidase (HRP), or even Fc domains from different species (Minibodies).
If you need a different functional molecule on them, please contact us with your idea.