TagFP Selector

Cat No: N0510 Category:


TagFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

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TagFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

The oriented and selective attachment via a flexible linker guarantees high accessibility of the sdAbs and largely eliminates batch-to-batch variations.

Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. TagFP Selector thus features high affinity and superior capacity for TagFP fusion proteins while showing negligible non-specific background.

TagFP Selector captures a wide range of “Tag” fluorescent derivatives ((e.g., TagBFP & TagRFP) . For more details and other fluorescent protein variants, please see our Specificity Table.

TagFP Selector is compatible with conventional physiological buffers and with more stringent compositions. As guidance, please look at some suggestions present in our IP protocol. TagFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

Conjugation Amount Cat No. RRID
4% cross-linked agarose 2000 μl N0510-L AB_3075942
Related Products:

GFP Selector

mNeonGreen Selector

RFP Selector

Selector Control beads

Clone: 1H7
Host: Alpaca
Produced in: E.coli
Application: IP
Dilution: N/A
Capacity: > 3 μg BFP per μl of packed beads
Antigen: -
Targets: tagBFP
Specificity: TagRFP, Tag BFP and mTagBFP2
Formulation: 50 % slurry in PBS containing 20 % Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Store at 4 °C for up to 12 months. Do not freeze!

Suggested protocols for IP and elutions using our Selector system can be found here: IP protocol

This and other protocols can be found on our Resources page.

• Common buffer substances at pH 5 to 9

• 2% Triton X-100, 1% Tween-20, 1% NP-40,
1% CHAPS, 1% Deoxycholate, 0.1% SDS

• 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA

• 4 M urea

• 10 mM DTT, 10 mM 2-Mercaptoethanol

• RNAse A, DNAse I, Benzonase, protease inhibitors

  1. Han Y, Georgii E, Priego-Cubero S, et al. Arabidopsis histone deacetylase HD2A and HD2B regulate seed dormancy by repressing DELAY OF GERMINATION 1. Front Plant Sci. 2023;14:1124899. Published 2023 May 29. doi:10.3389/fpls.2023.1124899 (CoIP; Arabidopsis)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. Limited Use Label License The purchaser of this product is granted a limited, non-transferable right to use the purchased material only for internal, academic research purposes. The license explicitly excludes the right to resell this product or any of its components. For information on further licensing possibilities please contact info@nano-tag.com
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