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GFP Selector

Cat No: N0310 Category:

275,00 € – 445,00 €

GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is site-specific and covalently immobilized on 4% cross-linked agarose or agarose magnetic beads.

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GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose or magnetic-agarose beads.

The oriented and selective attachment via a flexible linker to the beads guarantees high accessibility of the sdAbs and largely eliminates batch-to-batch variations.

Due to the single-chain nature of sdAbs and their covalent immobilization, no “leakage” of light or heavy chains from IgGs is observed during elution using SDS sample buffer. GFP Selector thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background.

GFP Selector captures a wide range of GFP derivatives. For more details and other fluorescent protein variants, please see our Specificity Table.

GFP Selector is compatible with conventional physiological buffers and with more stringent compositions. As guidance, please look at some suggestions present in our IP protocol. GFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

MSDS
Protocols
IP
Variations:
Conjugation Amount Cat No. RRID
4% cross-linked agarose 2000 μl N0310-L AB_3075911
4% cross-linked magnetic agarose 2000 μl N0315-L AB_3075912
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TagFP Selector

Selector Control beads
Clone: 1H1
Host: Alpaca
Produced in: E.coli
Application: IP
Dilution: -
Capacity: > 3 μg GFP per μl of packed beads
Antigen: -
Targets: GFP
Specificity: GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine. Other not tested.
Formulation: 50 % slurry in PBS containing 20 % Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Store at 4°C for up to 12 months. Do not freeze!
Protocols:

Suggested protocols for IP and elutions using our Selector system can be found here: IP protocol

This and other protocols can be found on our Resources page.

 

Buffer
Compatibility		 • Common buffer substances at pH 5 to 9

• 2% Triton X-100, 1% Tween-20, 1% NP-40,
1% CHAPS, 1% Deoxycholate, 0.1% SDS

• 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA

• 4 M urea

• 10 mM DTT, 10 mM 2-Mercaptoethanol

• RNAse A, DNAse I, Benzonase, protease inhibitors
References:
  1. Li Y, Kamiyama Y, Minegishi F, et al. Group C MAP kinases phosphorylate MBD10 to regulate ABA-induced leaf senescence in Arabidopsis. Plant J. Published online March 13, 2024. doi:10.1111/tpj.16706 (IP; Arabidopsis)
  2. Sanfilippo P, Kim AJ, Bhukel A, et al. Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome. Neuron. Published online January 13, 2024. doi:10.1016/j.neuron.2023.12.014 (CoIP; fruit fly)
  3. Devi S, Indramohan M, Jäger E, et al. CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout. Cell Rep. 2023;42(3):112265. doi:10.1016/j.celrep.2023.112265
  4. Dobson L, Barrell WB, Seraj Z, et al. GSK3 and lamellipodin balance lamellipodial protrusions and focal adhesion maturation in mouse neural crest migration. Cell Rep. 2023;42(9):113030. doi:10.1016/j.celrep.2023.113030
  5. Thölmann S, Seebach J, Otani T, et al. JAM-A interacts with α3β1 integrin and tetraspanins CD151 and CD9 to regulate collective cell migration of polarized epithelial cells. Cell Mol Life Sci. 2022;79(2):88. Published 2022 Jan 24. doi:10.1007/s00018-022-04140-5
  6. Fitieh A, Locke AJ, Mashayekhi F, Khaliqdina F, Sharma AK, Ismail IH. BMI-1 regulates DNA end resection and homologous recombination repair. Cell Rep. 2022;38(12):110536. doi:10.1016/j.celrep.2022.110536
  7. Ashraf APK, Gerke V. The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair. Front Cell Dev Biol. 2022;10:968164. Published 2022 Sep 19. doi:10.3389/fcell.2022.968164
  8. Futamata H, Fukuda M, Umeda R, et al. Cryo-EM structures of thermostabilized prestin provide mechanistic insights underlying outer hair cell electromotility [published correction appears in Nat Commun. 2022 Nov 28;13(1):7337]. Nat Commun. 2022;13(1):6208. Published 2022 Oct 20. doi:10.1038/s41467-022-34017-x
  9. Ibrahim Z, Wang T, Destaing O, et al. Structural insights into p300 regulation and acetylation-dependent genome organisation. Nat Commun. 2022;13(1):7759. Published 2022 Dec 15. doi:10.1038/s41467-022-35375-2
  10. Klama S, Hirsch AG, Schneider UM, Zander G, Seel A, Krebber H. A guard protein mediated quality control mechanism monitors 5′-capping of pre-mRNAs. Nucleic Acids Res. 2022;50(19):11301-11314. doi:10.1093/nar/gkac952
  11. Petutschnig E, Anders J, Stolze M, et al. EXTRA LARGE G-PROTEIN2 mediates cell death and hyperimmunity in the chitin elicitor receptor kinase 1-4 mutant. Plant Physiol. 2022;189(4):2413-2431. doi:10.1093/plphys/kiac214 (CoIP)
  12. Rathi S, Polat I, Pereira G. The budding yeast GSK-3 homologue Mck1 is an essential component of the spindle position checkpoint. Open Biol. 2022;12(11):220203. doi:10.1098/rsob.220203  (CoIP)
  13. Grosse S, Lu YY, Coban I, Neumann B, Krebber H. Nuclear SR-protein mediated mRNA quality control is continued in cytoplasmic nonsense-mediated decay. RNA Biol. 2021;18(10):1390-1407. doi:10.1080/15476286.2020.1851506
  14. Kojima T, Wasano K, Takahashi S, Homma K. Cell death-inducing cytotoxicity in truncated KCNQ4 variants associated with DFNA2 hearing loss. Dis Model Mech. 2021;14(11):dmm049015. doi:10.1242/dmm.049015 (CoIP)
  15. Hirsch AG, Becker D, Lamping JP, Krebber H. Unraveling the stepwise maturation of the yeast telomerase including a Cse1 and Mtr10 mediated quality control checkpoint. Sci Rep. 2021;11(1):22174. Published 2021 Nov 12. doi:10.1038/s41598-021-01599-3
  16. Ramsay EP, Abascal-Palacios G, Daiß JL, et al. Structure of human RNA polymerase III. Nat Commun. 2020;11(1):6409. Published 2020 Dec 17. doi:10.1038/s41467-020-20262-5
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. Limited Use Label License The purchaser of this product is granted a limited, non-transferable right to use the purchased material only for internal, academic research purposes. The license explicitly excludes the right to resell this product or any of its components. For information on further licensing possibilities please contact info@nano-tag.com
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