GFP Selector

Cat No: N0310 Category:

275,00 445,00 

GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is site-specific and covalently immobilized on 4% cross-linked agarose or agarose magnetic beads.

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GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose or magnetic-agarose beads.

The oriented and selective attachment via a flexible linker to the beads guarantees high accessibility of the sdAbs and largely eliminates batch-to-batch variations.

Due to the single-chain nature of sdAbs and their covalent immobilization, no “leakage” of light or heavy chains from IgGs is observed during elution using SDS sample buffer. GFP Selector thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background.

GFP Selector captures a wide range of GFP derivatives. For more details and other fluorescent protein variants, please see our Specificity Table.

GFP Selector is compatible with conventional physiological buffers and with more stringent compositions. As guidance, please look at some suggestions present in our IP protocol. GFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

Conjugation Amount Cat No. RRID
4% cross-linked agarose 2000 μl N0310-L AB_3075911
4% cross-linked magnetic agarose 2000 μl N0315-L AB_3075912
Related Products:

RFP Selector

mNeonGreen Selector

TagFP Selector

Selector Control beads

Clone: 1H1
Host: Alpaca
Produced in: E.coli
Application: IP
Dilution: -
Capacity: > 3 μg GFP per μl of packed beads
Antigen: -
Targets: GFP
Specificity: GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine. Other not tested.
Formulation: 50 % slurry in PBS containing 20 % Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Store at 4°C for up to 12 months. Do not freeze!

Suggested protocols for IP and elutions using our Selector system can be found here: IP protocol

This and other protocols can be found on our Resources page.


• Common buffer substances at pH 5 to 9

• 2% Triton X-100, 1% Tween-20, 1% NP-40,
1% CHAPS, 1% Deoxycholate, 0.1% SDS

• 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA

• 4 M urea

• 10 mM DTT, 10 mM 2-Mercaptoethanol

• RNAse A, DNAse I, Benzonase, protease inhibitors

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