250,00 € – 420,00 €
GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is site-specific and covalently immobilized on 4% cross-linked agarose or agarose magnetic beads.
GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose or magnetic-agarose beads.
The oriented and selective attachment via a flexible linker to the beads guarantees high accessibility of the sdAbs and largely eliminates batch-to-batch variations.
Due to the single-chain nature of sdAbs and their covalent immobilization, no “leakage” of light or heavy chains from IgGs is observed during elution using SDS sample buffer. GFP Selector thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background.
GFP Selector captures a wide range of GFP derivatives. For more details and other fluorescent protein variants, please see our Specificity Table.
GFP Selector is compatible with conventional physiological buffers and with more stringent compositions. As guidance, please look at some suggestions present in our IP protocol. GFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
|Capacity:||> 3 μg GFP per μl of packed beads|
|Specificity:||GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine. Other not tested.|
|Formulation:||50 % slurry in PBS containing 20 % Ethanol|
|Storing:||Store at 4°C for up to 12 months. Do not freeze!|
Suggested protocols for IP and elutions using our Selector system can be found here: IP protocol
This and other protocols can be found on our Resources page.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. Limited Use Label License The purchaser of this product is granted a limited, non-transferable right to use the purchased material only for internal, academic research purposes. The license explicitly excludes the right to resell this product or any of its components. For information on further licensing possibilities please contact email@example.com|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|