GFP Selector

Cat No: N0310 Category:

250,00 420,00 

GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

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GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations.

Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background.

GFP Selector immobilizes a wide range of GFP derivatives. For details see our Product Specificity Chart.

GFP Selector is compatible not only with physiological buffers but also with high stringency buffers (see Product Data Sheet). GFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

MSDS
Variations:
Conjugation Amount Cat No.
4% cross-linked agarose 2000 μl N0310-L
4% cross-linked magnetic agarose 2000 μl N0315-L
Related Products: -
Clone: 1H1
Host: Alpaca
Produced in: E.coli
Application: IP
Dilution: -
Capacity: > 2.5 μg GFP per μl of packed beads
Antigen: GFP
Specificity: GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine.
Formulation: 50 % slurry in PBS containing 20 % Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Store at 4°C for up to 12 months. Do not freeze!
Protocols: -
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.