400,00 €
FluoTag®-Q anti-TagFP is an in-house developed single-domain antibody (sdAb) with high affinity and specificity for fluorescent proteins derived from Entacmaea quadricolor, including TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate, and mKate2. It features one fluorophore per sdAb molecule for more quantitative measurements (Q).
Fluorescent proteins (FPs), widely used as protein tags in cell biology and microscopy, have revolutionized protein imaging and biochemical applications. TagRFP, a monomeric red fluorescent protein, was engineered from TurboRFP (derived from Entacmaea quadricolor) to exhibit an extended fluorescence lifetime and enhanced photostability (Merzlyak et al., 2007). Through further mutagenesis, this red-emitting protein was transformed into the blue-emitting TagBFP (Subach et al., 2008). A few years later, an improved version known as mTagBFP2 was introduced, offering greater brightness and stability (Subach et al., 2011). In 2007, mKate was developed from TagRFP, noted for its excellent far-red fluorescence and photostability (Shcherbo et al., 2007). The most currently available variants can be found on an open-source Fluorescent Protein Database FPbase.
FluoTag®-Q anti-TagFP binds strongly to TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate and mKate2. For detailed information, please refer to our FP Specificity Chart.
FluoTags® are single-domain antibodies (sdAbs) directly conjugated to a defined number of fluorophores. FluoTag®-Q is sdAb equipped with a single fluorophore for more quantitative readouts. FluoTag®-X2 carries two fluorophores per sdAb. FluoTag®-X4 is a blend of two different sdAbs that bind simultaneously to the single target, each bearing two fluorophores, thereby decorating the target protein with 4 fluorophores in total for a brighter signal. For more detailed information on FluoTags®, please check our Technology Section.
Variations: |
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Related Products: | - | ||||||||||||||||||||||||
Clone: | 1H7 | ||||||||||||||||||||||||
Host: | Alpaca | ||||||||||||||||||||||||
Produced in: | E.coli | ||||||||||||||||||||||||
Application: | IF | ||||||||||||||||||||||||
Dilution: | 1:1000 (corresponding to 5 nM final concentration) | ||||||||||||||||||||||||
Capacity: | N/A | ||||||||||||||||||||||||
Antigen: | - | ||||||||||||||||||||||||
Targets: | TagFP | ||||||||||||||||||||||||
Specificity: | Recognizes TagRFP, TagRFP657, TagBFP, mTagBFP2, mKate and mKate2. Likely reactive with other untested TagRFP derivatives. | ||||||||||||||||||||||||
Formulation: |
The sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 200 µl of 50% glycerol in deionized water. We recommend including 0.1% sodium azide as a preservative, if applicable. When reconstituted with 200 µl, the final concentration of single-domain antibody is 5 µM . |
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kDa: | - | ||||||||||||||||||||||||
Ext Coef: | - | ||||||||||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||||||||||
Storing: |
Vials containing lyophilized protein can be stored at 4°C for 6 months. We recommend reconstituting the protein with 50% sterile glycerol in deionized water and including 0.1 % sodium azide as a preservative, if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long-term storage at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4°C. |
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Protocols: |
These products are not recommended for detecting proteins in Western blot, as sdAbs primarily recognize native or folded proteins. For more details check our Protocols in Resource Section and FP Specificity Chart. |
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References: |
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Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||||||||
Legal terms: | By purchasing this product, you agree to our general Terms & Conditions. |