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Indirect immunostaining of PFA fixed Hela cells expressing Nup98-GFP with FluoTag®-X4 Atto 565 anti-GFP sdAb (Cat. No. N0304, dilution 1:500, the GFP signal is represented in green, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in yellow).

FluoTag®-X4 anti-GFP

Cat No: N0304 Category:

375,00 €

FluoTag®-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity.

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General information

A breakthrough in biology and bioluminescence began in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants in the fluorescence protein database “fpbase”

Our sdAbs bind strongly to most of the fluorescent proteins derived from Aequorea Victoria; check our specificity chart in our Resource Section.

For detailed information regarding our FluoTag-Q, -X2, and -X4 series check our Technology Section.

MSDS Protocols
Variations:
Amount Conjugation Cat No.
Atto488 200 μl N0304-At488-L
AZDye568 200 μl N0304-AF568-L
Atto643 200 μl N0304-At643-L
Alexa647 200 μl N0304-AF647-L
AbberiorStar635P 200 μl N0304-Ab635P-L
Related Products:

sdAb anti-GFP HRP (cat. no. N0305-HRP)

sdAb anti-GFP Biotin (cat. no. N0305-Biotin)

 
Clone: 1H1, 1B2
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:250 (corresponding to 5 nM for each sdAb clone)
Capacity: N/A
Antigen: -
Targets: GFP
Specificity: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine.
Formulation: A mixture of two sdAb clones lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted in 200 µl, the concentration of each individual single-domain antibody is 1.25 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

This Product is not recommended to be used to detect proteins in Western Blott, sdAbs tend to recognize mainly native/folded proteins.

Look at detailed protocols and our specificity chart in our Resource Section.

 

 
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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