Indirect immunostaining of PFA fixed Hela cells expressing Nup98-GFP with FluoTag®-X4 Atto 565 anti-GFP sdAb (Cat. No. N0304, dilution 1:500, the GFP signal is represented in green, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in yellow).

PFA-fixed Cos7 cells expressing a TOM70-nfGFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-GFP coupled to Alexa Fluor 647 (Cat. No. N0304-AF647, dilution 1:500). A Greyscale image of the staining performed with N0304-AF647. B False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfGFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-GFP coupled to AZDye568 (Cat. No. N0304-AF568, dilution 1:500). A Greyscale image of the staining displayed with N0304-AF568. B False color representation of the image shown in A is presented in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfGFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-GFP coupled to Atto 488 (Cat. No. N0304-At488, dilution 1:500). A Greyscale image of the staining performed with N0304-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.

FluoTag®-X4 anti-GFP

Name: FluoTag®-X4 anti-GFP
SKU: N0304
Price: 400.00 EUR
Availability: InStock

Cat No: N0304 Category: Primary sdAbs

400,00 €

FluoTag®-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity.

Clear

General information

A breakthrough in biology and bioluminescence began in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants in the fluorescence protein database “fpbase”

Our sdAbs bind strongly to most of the fluorescent proteins derived from Aequorea Victoria; check our specificity chart in our Resource Section.

For detailed information regarding our FluoTag-Q, -X2, and -X4 series check our Technology Section.

MSDS

Variations:

Conjugation	Amount	Cat No.	RRID
Atto488	200 μl	N0304-At488-L	AB_2744629
AZDye568	200 μl	N0304-AF568-L	AB_3075903
Atto643	200 μl	N0304-At643-L	AB_3075906
Alexa647	200 μl	N0304-AF647-L	AB_2905517
AbberiorStar635P	200 μl	N0304-Ab635P-L	AB_3075902

FluoTag®-X4 anti-GFP

General information

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