Immunostaining of PFA fixed 3T3 cells expressing a TOM70-mCherry reporter protein with FluoTag®-Q Atto 488 anti-RFP sdAb (Cat. No. N0401-At488-L, dilution 1:500, the mCherry signal is represented in red, the corresponding FluoTag-signal is represented in green and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue).

PFA-fixed Cos7 cells expressing a TOM70-nfRFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-Q anti-RFP coupled to Alexa Fluor 647 (Cat. No. N0401-AF647, dilution 1:500). A Greyscale image of the staining performed with N0401-AF647. B False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfRFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-Q anti-RFP coupled to AZDye 568 (Cat. No. N0401-AF568, dilution 1:500). A Greyscale image of the staining performed with N0401-AF568. B False color representation of the image shown in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfRFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-Q anti-RFP coupled to Atto 488 (Cat. No. N0401-At488, dilution 1:500). A Greyscale image of the staining performed with N0401-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding BFP signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.

FluoTag®-Q anti-RFP

Name: FluoTag®-Q anti-RFP
SKU: N0401
Price: 400.00 EUR
Availability: InStock

Cat No: N0401 Category: Primary sdAbs

400,00 €

FluoTag®-Q anti-RFP is derived from an in-house developed single-domain antibody (sdAb) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity.

Clear

One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants in the fluorescence protein database “fpbase”

Our nanobodies bind specifically and strongly to DsRed, RFP, and mCherry, among other family members. Look at our specificity chart in the Resource Section.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS

Variations:

Conjugation	Amount	Cat No.	RRID
Atto488	200 μl	N0401-At488-L	AB_3075917
AZDye568	200 μl	N0401-AF568-L	AB_3075915
Atto643	200 μl	N0401-At643-L	AB_3075918
Alexa647	200 μl	N0401-AF647-L	AB_2905529
AbberiorStar635P	200 μl	N0401-Ab635P-L	AB_2744602

FluoTag®-Q anti-RFP

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