Immunostaining of PFA fixed 3T3 cells expressing a TOM70-mCherry reporter protein with FluoTag®-X4 Atto 647N anti-RFP sdAb (Cat. No. N0404, dilution 1:500, the mCherry signal is represented in red, the corresponding FluoTag-signal is represented in green and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue).
FluoTag®-X4 anti-RFP is a blend of two in-house developed single-domain antibodies (sdAbs) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity.
One of the first red-emitting fluorescent proteins described was DsRed from Discosoma sp. After a couple of years from its publication in 1999 and more than 20 mutations, a monomeric version with several enhanced characteristics appeared as mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, being mCherry one of the most popular derivatives.
Our nanobodies bind specifically and strongly to DsRed, RFP, and mCherry, among others family members. Look at our specificity chart in the resource section.
For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.
|Specificity:||mRFP (red fluorescent protein) and other derivatives like mOrange, dsRed1, dsRED2, tdTomato, mCherry and mScarlet-i.|
|Formulation:||Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
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