Immunostaining of PFA fixed 3T3 cells expressing a TOM70-mCherry reporter protein with FluoTag®-X4 Atto 647N anti-RFP sdAb (Cat. No. N0404, dilution 1:500, the mCherry signal is represented in red, the corresponding FluoTag-signal is represented in green and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue).

A single motoneuron in the Drosophila larval central nervous system, fixed with 2% PFA. Cell membrane (RFP,red) and synaptic connections (YPet, blue), were stained with FluoTag-X4 anti-RFP (N0404-AF568-L) and FluoTag-X4 anti-GFP (N0304-AT488).

Image credit: Tom Pettini, Matthias Landgraf Lab, University of Cambridge, UK

PFA-fixed Cos7 cells expressing a TOM70-nfRFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-RFP coupled to Alexa Fluor 647 (Cat. No. N0404-AF647, dilution 1:500). A Greyscale image of the staining performed with N0404-AF647. B False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfRFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-RFP coupled to AZDye 568 (Cat. No. N0404-AF568, dilution 1:500). A Greyscale image of the staining performed with N0404-AF568. B False color representation of the image shown in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfRFP-BFP fusion protein (nf: non-fluorescent) were stained with FluoTag®-X4 anti-RFP coupled to Atto 488 (Cat. No. N0404-At488, dilution 1:500). A Greyscale image of the staining performed with N0404-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.