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RFP Selector

Cat No: N0410 Category:

275,00 € – 445,00 €

RFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose or magnetic-agarose beads.

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RFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose or magnetic-agarose beads.

The oriented and selective attachment via a flexible linker guarantees high accessibility of the sdAbs and largely eliminates batch-to-batch variations.

Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. RFP Selector thus features high affinity and superior capacity for RFP fusion proteins while showing negligible non-specific background.

RFP Selector captures a wide range of RFP derivatives. For more details and other fluorescent protein variants, please see our Specificity Table.

RFP Selector is compatible with conventional physiological buffers and with more stringent compositions. As guidance, please look at some suggestions present in our IP protocol. RFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
4% cross-linked magnetic agarose 2000 μl N0415-L AB_3075928
4% cross-linked agarose 2000 μl N0410-L AB_3075927
Related Products:

GFP Selector

mNeonGreen Selector

TagFP Selector

Selector Control beads
Clone: 2B12
Host: Alpaca
Produced in: E.coli
Application: IP
Dilution: -
Capacity: > 3 μg RFP per μl of packed beads
Antigen: -
Targets: mRFP
Specificity: mRFP (red fluorescent protein) and other derivatives like mOrange, dsRed1, dsRED2, tdTomato, mCherry and mScarlet-i.
Formulation: 50 % slurry in PBS containing 20 % Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Store at 4°C for up to 12 months. Do not freeze!
Protocols:

Suggested protocols for IP and elutions using our Selector system can be found here: IP protocol

This and other protocols can be found on our Resources page.

Buffer
Compatibility		 • Common buffer substances at pH 5 to 9

• 2% Triton X-100, 1% Tween-20, 1% NP-40,
1% CHAPS, 1% Deoxycholate, 0.1% SDS

• 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA

• 4 M urea

• 10 mM DTT, 10 mM 2-Mercaptoethanol

• RNAse A, DNAse I, Benzonase, protease inhibitors
References:
  1. Kojima T, Wasano K, Takahashi S, Homma K. Cell death-inducing cytotoxicity in truncated KCNQ4 variants associated with DFNA2 hearing loss. Dis Model Mech. 2021;14(11):dmm049015. doi:10.1242/dmm.049015 (CoIP)
  2. Thornton DT. Characterization of HSP90 subpopulation conformations targeted by inhibitors and the effect of phosphorylation on HSP90’s association with co-chaperone and client proteins. Master’s Thesis (2019). University of New Hampshire.
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. Limited Use Label License The purchaser of this product is granted a limited, non-transferable right to use the purchased material only for internal, academic research purposes. The license explicitly excludes the right to resell this product or any of its components. For information on further licensing possibilities please contact info@nano-tag.com
Legal terms: By purchasing this product you agree to our general terms and conditions.

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