Liberate your IF: Species-independent multitarget stainings!
Due to the combination of unique features like monovalent binding, highly specific, and very low dissociation of the target (low Koff), it is possible with our FluoTags® to perform One-step indirect immunofluorescence using your favorite conventional primary antibodies. This capability of the pre-mixing primary antibody with species and isotype-specific FluoTags® also allows easy multiplex of all your immunofluorescence applications without significant problems. There is no need to compromise your immunofluorescence-based application by selecting suboptimal primaries from different species for multitarget labeling. Our approach makes your stainings independent of the species independent. In other words, immunostaining experiments not only can become shorter by a one-step IF, but it is also possible to use on the same sample multiple IgG1 monoclonals raised on mice or multiple polyclonal antibodies raised in rabbits (Caution here: polyclonal primary antibodies need to be affinity purified for best results).
For further clarifications, you can spend two minutes on our video tutorial (below) or go directly to the specifics of our proposed protocol here