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MBP Selector

Cat No: N0110 Category:

275,00 €

MBP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

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MBP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations.

Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. MBP Selector thus features high affinity and superior capacity for MBP fusion proteins while showing negligible non-specific background.

MBP Selector is compatible with conventional physiological buffers and with more stringent compositions. As guidance, please look at some suggestions present in our IP protocol. MBP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
4% cross-linked agarose 2000 μl N0110-L AB_3075895
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Clone: 1G5
Host: Alpaca
Produced in: E. coli
Application: IP
Dilution: N/A
Capacity: > 2.5 μg MBP per μl of packed beads
Antigen: -
Targets: MBP
Specificity: Recognizes E.coli maltose-binding protein (MBP)
Formulation:

50 % slurry in PBS containing 20 % Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Store at 4°C for up to 12 months. Do not freeze!
Protocols:

Suggested protocols for IP and elutions using our Selector system can be found here: IP protocol

This and other protocols can be found on our Resources page.
References:
  1. James C, Möller U, Spillner C, et al. Phosphorylation of ELYS promotes its interaction with VAPB at decondensing chromosomes during mitosis. EMBO Rep. 2024;25(5):2391-2417. doi:10.1038/s44319-024-00125-6 (Binding assay; E.coli)
  2. Pörschke M, Rodríguez-González I, Parfentev I, Urlaub H, Kehlenbach RH. Transportin 1 is a major nuclear import receptor of the nitric oxide synthase interacting protein. J Biol Chem. 2023;299(3):102932. doi:10.1016/j.jbc.2023.102932
  3. Baade I, Hutten S, Sternburg EL, et al. The RNA-binding protein FUS is chaperoned and imported into the nucleus by a network of import receptors. J Biol Chem. 2021;296:100659. doi:10.1016/j.jbc.2021.100659
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.