One vial contains 10 mg of ALFA elution peptide to prepare a native elution buffer for the ALFA Selector PE.
One vial contains 10 mg of ALFA elution peptide to prepare a native elution buffer for the ALFA Selector PE. We recommend dissolving the lyophilized peptide in 250 µL pure water to get a 100x stock solution. This peptide will not efficiently elute ALFA-tagged proteins from the ALFA Selector ST (super tight).
PLEASE NOTE: Every unit of ALFA Selector PE is delivered including a vial containing 10 mg lyophilized peptide (sufficient for 25 mL elution buffer). This is the right product for you if you need more elution peptides for your experiments.
|Produced in:||Synthetic peptide|
|Specificity:||Is recognized by NbALFA, NbALFA-PE and NbALFA-CE|
|Formulation:||Lyophilized powder containing 10 mg ALFA elution peptide. Reconstitute with 250 µL sterile water to obtain a 100x stock solution (20 mM).|
|Storing:||Vials containing lyophilized peptide can be stored at 4 °C for up to 6 months. When reconstituted in water, aliquot and store at -20°C or below.|
Please dissolve the lyophilized peptide (10 mg) in 250 µl of distilled water. This results in an ALFA peptide concentration of 40 mg/ml equivalent to 20 mM (100x).
Detail protocols for IP and elutions using the ALFA system can be found here in the ALFA Selector Manual
Protocols can be found on our Resources page.
Götzke et al. 2019 The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|