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ALFA elution peptide

Cat No: N1520 Categories: ,

145,00 €

One vial contains 10 mg of ALFA elution peptide to prepare a native elution buffer for the ALFA Selector PE.

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One vial contains 10 mg of ALFA elution peptide to prepare a native elution buffer for the ALFA Selector PE. We recommend dissolving the lyophilized peptide in 250 µL pure water to get a 100x stock solution. This peptide will not efficiently elute ALFA-tagged proteins from the ALFA Selector ST (super tight).

PLEASE NOTE: Every unit of ALFA Selector PE is delivered including a vial containing 10 mg lyophilized peptide (sufficient for 25 mL elution buffer). This is the right product for you if you need more elution peptides for your experiments.

MSDS
Protocols
ALFA Selector Manual
Variations:
Conjugation Amount Cat No. RRID
- 10 mg N1520 -
Related Products: -
Clone: -
Host: -
Produced in: Synthetic peptide
Application:
Dilution: -
Capacity: -
Antigen: -
Targets: ALFA-tag
Specificity: Is recognized by NbALFA, NbALFA-PE and NbALFA-CE
Formulation: Lyophilized powder containing 10 mg ALFA elution peptide. Reconstitute with 250 µL sterile water to obtain a 100x stock solution (20 mM).
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized peptide can be stored at 4 °C for up to 6 months. When reconstituted in water, aliquot and store at -20°C or below.
Protocols:

Please dissolve the lyophilized peptide (10 mg) in 250 µl of distilled water. This results in an ALFA peptide concentration of 40 mg/ml equivalent to 20 mM (100x).

Detail protocols for IP and elutions using the ALFA system can be found here in the ALFA Selector Manual.

Protocols can be found on our Resources page.
References:
  1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
  2. van Zwam MC, Dhar A, Bosman W, et al. IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms. PLoS Biol. 2024;22(3):e3002551. Published 2024 Mar 11. doi:10.1371/journal.pbio.3002551 (CoIP; HT1080 cells)
  3. Krauter D, Stausberg D, Hartmann TJ, et al. Targeting PI3K/Akt/mTOR signaling in rodent models of PMP22 gene-dosage diseases. EMBO Mol Med. Published online February 21, 2024. doi:10.1038/s44321-023-00019-5  (IP; rat sciatic nerve, HEK293T)
  4. Lin C, Feng S, DeOliveira CC, Crane BR. Cryptochrome-Timeless structure reveals circadian clock timing mechanisms. Nature. 2023;617(7959):194-199. doi:10.1038/s41586-023-06009-4 (CoIP; fruit fly S2 cells)
  5. Tao X, Zhao C, MacKinnon R. Membrane protein isolation and structure determination in cell-derived membrane vesicles. Proc Natl Acad Sci U S A. 2023;120(18):e2302325120. doi:10.1073/pnas.2302325120 (vesicle purification; Sf9 cells)
  6. Guilhen C, Lima WC, Ifrid E, Crespo-Yañez X, Lamrabet O, Cosson P. A New Family of Bacteriolytic Proteins in Dictyostelium discoideum. Front Cell Infect Microbiol. 2021;10:617310. Published 2021 Feb 3. doi:10.3389/fcimb.2020.617310 (IP; D. discoideum)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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