ALFA Selector ST

Cat No: N1511 Category:

250,00 420,00 

ALFA Selector ST (for “Super Tight”) is an innovative, high-affinity resin (Agarose & Magnetic) designed for efficient and clean pull-down of ALFA-tagged proteins.

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ALFA Selector ST (for “Super Tight”) is an innovative, high-affinity resin designed for the highly efficient pull-down of ALFA-tagged proteins.

Please look to our Technology Section for more information about the ALFA system.

ALFA Selector ST is based on a single-domain antibody (sdAb), which is covalently immobilized on 4% cross-linked agarose or agarose-magnetic beads and binds ALFA-tagged proteins with extraordinarily high affinity (Kd = 26 pM). Target proteins are efficiently released from ALFA Selector ST under acidic (e.g., 0.1 M glycin pH2.2) or denaturing conditions. The extremely tight interaction with ALFA-tagged proteins makes competitive elution using ALFA elution peptide inefficient. We recommend using ALFA Selector PE for competitive elution under physiological and room temperature condition conditions. For efficient elutions at 4°C, we recommend using the ALFA Selector CE

The innovative, oriented and highly selective attachment of the active sdAbs via a flexible linker guarantees optimal accessibility for target proteins and at the same time largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed even during elution with strongly denaturing and reducing conditions (e.g., SDS sample buffer). ALFA Selector ST thus features high affinity and superior capacity for ALFA fusion proteins while showing negligible non-specific background.

ALFA Selector ST is compatible not only with physiological buffers but also with high stringency buffers (see ALFA Selector Manual). ALFA Selector ST thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

MSDS
Variations:
Conjugation Amount Cat No.
4% cross-linked magnetic agarose 2000 μl N1516
4% cross-linked agarose 2000 μl N1511-L
Related Products:

ALFA Selector PE (Peptide Elutable), magnetic and agarose

ALFA Selector CE (Cold Elutable), magnetic and agarose

ALFA elution peptide (10 mg)

 

Clone: 1G5
Host: Alpaca
Produced in: E.coli
Application: IP
Dilution: N/A
Capacity: > 4 µg ALFA-tagged GFP per µL of packed beads
Antigen: ALFA-tag
Specificity: Recognizes the ALFA-tag (SRLEEELRRRLTE)
Formulation: Resin is 50 % slurry in PBS containing 20 % Ethanol.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Store at 4 °C for up to 12 months. Do not freeze!
Protocols:

Detail protocols for IP and elutions using the ALFA system can be found here in the ALFA Selector Manual 

Protocols can be found on our Resources page.

References:

Götzke et al. 2019 The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.

Kilisch et al. 2021 Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer.

Supplementary Information for Kilisch et al. 2021

 

Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.