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Ubiquitin K48 Selector

Cat No: N1810 Categories: ,

290,00 €

Ubiquitin K48 Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

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Ubiquitination is a post-translational modification in which an E3 ligase catalyzes the attachment of ubiquitin to a substrate protein. After the initial ubiquitin is conjugated, additional ubiquitin molecules can be linked through one of the seven lysine (K) residues in ubiquitin to form polyubiquitin chains. Linkage through lysine 48 (K48) serves as a canonical signal for proteasomal degradation, marking the substrate for recognition and processing by the 26S proteasome.

Ubiquitin K48 Selector is based on a single-domain antibody (sdAb), covalently immobilized on agarose beads, that specifically recognizes K48-linked polyubiquitin chains. It can be used in immunoprecipitation for the selective enrichment of K48-ubiquitinated proteins.

This product was a co-development with

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
4% cross-linked agarose 2 mL slurry N1810 AB_3076013
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Clone: A4-1
Host: Synthetic Library
Produced in: E.coli
Application: IP
Dilution: N/A
Capacity: > 2.5 µg K-48 linked Ubiquitin per µL of packed beads
Antigen: -
Targets: Ubiquitin K48 linkage
Specificity: Recognizes K48-linkages of polyubiquitin.
Formulation:

50% slurry in PBS containing 20% Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Store at 4°C for up to 12 months. Do not freeze!
Protocols: -
References:
  1. Weyer Y, Schwabl SI, Tang X, et al. The Dsc ubiquitin ligase complex identifies transmembrane degrons to degrade orphaned proteins at the Golgi. Nat Commun. 2024;15(1):9257. Published 2024 Oct 26. doi:10.1038/s41467-024-53676-6 (IP; yeast)
  2. Gonzalez-Santamarta M, Ceccato L, Carvalho AS, Rain JC, Matthiesen R, Rodriguez MS. Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome Using Chain-Specific Nanobodies. Methods Mol Biol. 2023;2602:125-136. doi:10.1007/978-1-0716-2859-1_9 (Isolation of ubiquitylated proteins, MS; MCL cells)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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