mNeonGreen Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.
mNeonGreen Selector is based on a high-affinity single-domain antibody (sdAb) that binds strongly and selectively mNeonGreen (Specificity Table) and is covalently immobilized on 4% cross-linked agarose beads.
The oriented and selective immobilization via a flexible linker guarantees high accessibility of the sdAbs and largely eliminates batch-to-batch variations.
Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. mNeonGreen Selector thus features high affinity and superior capacity for mNeonGreen fusion proteins while showing negligible non-specific background.
mNeonGreen Selector captures selectively mNeonGreen protein. For more details and other fluorescent protein variants, please see our Specificity Table.
mNeonGreen Selector is compatible with conventional physiological buffers and with more stringent compositions. As guidance, please look at some suggestions present in our IP protocol for the Selector series. mNeonGreen Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
|Capacity:||> 3 μg mNeonGreen per μl of packed beads|
|Specificity:||Recognizes mNeonGreen. Does not cross-react with any GFP-, dsRed, or TagBFP derivatives.|
|Formulation:||50 % slurry in PBS containing 20 % Ethanol|
|Storing:||Store at 4°C for up to 12 months. Do not freeze!|
Suggested protocols for IP and elutions using our Selector system can be found here: IP protocol
This and other protocols can be found on our Resources page.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|