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DNA PAINT image of nucelar pore complexes using sdAb anti-GFP DBCO (N0305-DBCO) modified with DNA oligos. Courtesy of MASSIVE PHOTONICS.

sdAb anti-GFP

Cat No: N0305 Category:

275,00 € – 625,00 €

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A breakthrough in the field of biology and bioluminescence started in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants here.

Our sdAbs bind strongly to most of the fluorescent proteins derived from Aequorea Victoria; check our specificity chart in the resources section.

 

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
HRP 200 μl N0305-HRP AB_3075910
unconjugated C-terminal Cys 250 μg N0305-250ug AB_3075907
Biotin 250 μg N0305-Biotin AB_3075908
DBCO 250 μg N0305-DBCO AB_3075909
Related Products: -
Clone: 1H1
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: GFP
Specificity: GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine.
Formulation: DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyohilized from 10 mM KPi,150 mM NaCl, 1 mM EDTA pH 6.0. Reconsitute with 250 µL pure water for immidate use or with 250 µL 50% glycerol for storage at -80 °C. The N0305-HRP is lyophilized from 200 µL HRP protector buffer and should be reconstituted with 200 µL pure deionized water. and Store at 4°C.
kDa: 16.1
Ext Coef: 24138
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for upto 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart

MSDS datasheets for HRP conjugated sdAbs can be found here

 
References:
  1. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF; DNA-PAINT)
  2. Niederauer C, Nguyen C, Wang-Henderson M, et al. Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions. Nat Commun. 2023;14(1):4345. Published 2023 Jul 19. doi:10.1038/s41467-023-40065-8 (DNA-PAINT)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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