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Multiplexing (see our Technologies): Indirect immunostaining of PFA fixed mouse hippocampus neurons with an anti-Tau primary antibody (314 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 565 anti-msIgG2 sdAb (Cat. No. 2702-At565-S, represented in red) and an anti-Synaptoporin primary antibody (102 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 488 anti-msIgG2 sdAb (Cat. No. 2702-At488-S, represented in green). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X2 anti-Mouse IgG2a/b

Cat No: N2702 Category:

205,00 €

FluoTag®-X2 anti-Mouse IgG2 (Smart Secondaries®) is a species-specific FluoTag® directed against IgG2a and IgG2b subtype from Mouse. It serves as an effective secondary tool, enabling OneStep Immunofluorescence (IF) and species-independent Multiplexing.

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In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).

FluoTag®-X2 anti-Mouse IgG2a/b is a fluorescent single-domain antibody (sdAb) that binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain. As part of our Smart Secondaries® range, the FluoTag®-X2 anti-Mouse IgG1 is particularly suited for OneStep Immunofluorescence (OneStep IF) and species-independent Multiplexing.

FluoTag®-X2 anti-Mouse IgG2a/b is site-specifically conjugated to two fluorophores per sdAb (FluoTag®–X2 variant). To learn more about FluoTags®, please visit our Technology section here.

We provide this product conjugated to various fluorophores and offer custom conjugation services with most commercially available fluorophores, provided these are available in a thiol-reactive activated form.

MSDS
Protocols
Reconstitution and Storage
Multiplexing (MX-IF)
Variations:
Conjugation Amount Cat No. RRID
Janelia Fluor 635b 500 μL N2702-JF635B-S AB_3713467
Atto488 500 μL N2702-At488-S AB_3076053
AZDye568 500 μL N2702-AF568-S AB_3076052
Atto643 500 μL N2702-At643-S AB_3076054
Alexa647 500 μL N2702-AF647-S AB_2936181
LI-COR IRDye 680RD 500 μL N2702-Li680-S AB_3076055
LI-COR IRDye 800CW 500 μL N2702-Li800-S AB_3076056
abberior STAR 635P 500 μL N2702-Ab635P-S AB_3076051
Related Products:

FluoTag®-X2 anti-Mouse IgG1

FluoTag®-X2 anti-Chicken IgY

FluoTag®-X2 anti-Rabbit IgG

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Clone: 14A4
Host: Alpaca
Produced in: E.coli
Application: IF, WB
Dilution: 1:500 (corresponding to 10 nM final concentration if the sdAb was reconstituted as proposed below)
Capacity: N/A
Antigen: -
Targets: Mouse IgG2
Specificity: Recognizes native Mouse IgG2a and IgG2b. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation:

The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50% glycerol in deionized water. We recommend including 0.1% sodium azide as a preservative if applicable. When reconstituted with 500 µL, the single-domain antibody concentration is 5 µM. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (ICC/IF; tested species: rat)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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