Multiplexing (see our Technologies): Indirect immunostaining of PFA fixed mouse hippocampus neurons with an anti-Tau primary antibody (314 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 565 anti-msIgG2 sdAb (Cat. No. 2702-At565-S, represented in red) and an anti-Synaptoporin primary antibody (102 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 488 anti-msIgG2 sdAb (Cat. No. 2702-At488-S, represented in green). Nuclei were visualized by DAPI staining (blue).
In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).
Our anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.
FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.
|Dilution:||1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)|
|Specificity:||Recognizes native Mouse IgG2a and IgG2b. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.|
|Formulation:||The single sdAb clone was lyophilized from PBS pH 7.4. Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Suggested general protocols for immunofluorescence can be found in our Resource Section.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|