170,00 €
FluoTag®-X2 anti-Mouse IgG2 is a species-specific FluoTag® directed against IgG2a and IgG2b subtype from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications
In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).
Our anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.
FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.
Variations: |
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Clone: | 14A4 | ||||||||||||||||||||||||
Host: | Alpaca | ||||||||||||||||||||||||
Produced in: | E.coli | ||||||||||||||||||||||||
Application: | IF | ||||||||||||||||||||||||
Dilution: | 1:500 (corresponding to 10 nM final concentration if the sdAb was reconstituted as proposed bellow) | ||||||||||||||||||||||||
Capacity: | N/A | ||||||||||||||||||||||||
Antigen: | Mouse IgG2 | ||||||||||||||||||||||||
Specificity: | Recognizes native Mouse IgG2a and IgG2b. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs. | ||||||||||||||||||||||||
Formulation: | The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the single-domain antibody concentration is 5 µM. | ||||||||||||||||||||||||
kDa: | - | ||||||||||||||||||||||||
Ext Coef: | - | ||||||||||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||||||||||
Storing: | Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C. | ||||||||||||||||||||||||
Protocols: |
Suggested general protocols for immunofluorescence can be found in our Resource Section. One-Step immunofluorescence and multiplex staining can be performed with our smart secondaries. Here you can find more on these technologies One-Step-IF & Multiplexing |
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References: | - | ||||||||||||||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |