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Multiplexing (see our Technologies): Indirect immunostaining of PFA fixed mouse hippocampus neurons with an anti-Tau primary antibody (314 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 565 anti-msIgG2 sdAb (Cat. No. 2702-At565-S, represented in red) and an anti-Synaptoporin primary antibody (102 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 488 anti-msIgG2 sdAb (Cat. No. 2702-At488-S, represented in green). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X2 anti-Mouse IgG2a/b

Cat No: N2702 Category:

195,00 €

FluoTag®-X2 anti-Mouse IgG2 is a species-specific FluoTag® directed against IgG2a and IgG2b subtype from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications

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In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).

Our anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.

 

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μl N2702-At488-S AB_3076053
AZDye568 500 μl N2702-AF568-S AB_3076052
Atto643 500 μl N2702-At643-S AB_3076054
Alexa647 500 μl N2702-AF647-S AB_2936181
LI-COR IRDye 680RD 500 μl N2702-Li680-S AB_3076055
LI-COR IRDye 800CW 500 μl N2702-Li800-S AB_3076056
AbberiorStar635P 500 μl N2702-Ab635P-S AB_3076051
Related Products:

FluoTag®-X2 anti-Mouse IgG1

FluoTag®-X2 anti-Chicken IgY

FluoTag®-X2 anti-Rabbit IgG
Clone: 14A4
Host: Alpaca
Produced in: E.coli
Application: IF, WB
Dilution: 1:500 (corresponding to 10 nM final concentration if the sdAb was reconstituted as proposed bellow)
Capacity: N/A
Antigen: -
Targets: Mouse IgG2
Specificity: Recognizes native Mouse IgG2a and IgG2b. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the single-domain antibody concentration is 5 µM.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Suggested general protocols for immunofluorescence can be found in our Resource Section.

One-Step immunofluorescence and multiplex staining can be performed with our smart secondaries. Here you can find more on these technologies One-Step-IF & Multiplexing
References:
  1. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (ICC/IF; tested species: rat)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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