Multiplexing (see our Technologies): Indirect immunostaining of PFA fixed mouse hippocampus neurons with an anti-MAP2 primary antibody (188 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 488 anti-msIgG1 sdAb (Cat. No. 2002, represented in red), an anti-GFAP primary antibody (173 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 647N anti-msIgG1 sdAb (Cat. No. 2002, represented in green) and an anti-Synapsin1 primary antibody (106 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 565 anti-msIgG1 sdAb (Cat. No. 2002, represented in yellow). Nuclei were visualized by DAPI staining (blue).
In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.
Our anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.
FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.
|Dilution:||1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)|
|Specificity:||Recognizes the native Mouse IgG1 subtype. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.|
|Formulation:||The single sdAb clone was lyophilized from PBS pH 7.4. Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Suggested general protocols for immunofluorescence can be found in our Resource Section.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|