195,00 €
FluoTag®-X2 anti-Mouse IgG1 (Smart Secondaries®) is a species-specific FluoTag® directed against IgG1 from Mouse. It serves as an effective secondary tool, enabling One-step immunofluorescence (IF) and species-independent Multiplexing.
In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.
FluoTag®-X2 anti-Mouse IgG1 is a fluorescent single-domain antibody (sdAb) that binds specifically and strongly to mouse IgG1, independently of the IgGs light chains. As part of our Smart Secondaries® range, the FluoTag®-X2 anti-Mouse IgG1 is particularly suited for One-step immunofluorescence (One-step IF) and species-independent Multiplexing.
FluoTag®-X2 anti-Mouse IgG1 is site-specifically conjugated to two fluorophores per sdAb (FluoTag®–X2 variant). To learn more about FluoTags®, please visit our Technology section here.
We provide this product conjugated to various fluorophores and offer custom conjugation services with most commercially available fluorophores, provided these are available in a thiol-reactive activated form.
Variations: |
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Clone: | 10A4 | ||||||||||||||||||||||||||||||||
Host: | Alpaca | ||||||||||||||||||||||||||||||||
Produced in: | E.coli | ||||||||||||||||||||||||||||||||
Application: |
IF (ICC), IHC, WB |
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Dilution: | 1:500 (corresponding to 10 nM final concentration if the sdAb was reconstituted as proposed bellow) | ||||||||||||||||||||||||||||||||
Capacity: | N/A | ||||||||||||||||||||||||||||||||
Antigen: | - | ||||||||||||||||||||||||||||||||
Targets: | Mouse IgG1 | ||||||||||||||||||||||||||||||||
Specificity: | Recognizes the native Mouse IgG1 subtype. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs. | ||||||||||||||||||||||||||||||||
Formulation: |
The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50% glycerol in deionized water. We recommend including 0.1% sodium azide as a preservative if applicable. When reconstituted with 500 µL, the single-domain antibody concentration is 5 µM. For more details, click the Protocols button above and check Reconstitution and Storage. |
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kDa: | - | ||||||||||||||||||||||||||||||||
Ext Coef: | - | ||||||||||||||||||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
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Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page. |
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References: |
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Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |