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Multiplexing (see our Technologies): Indirect immunostaining of PFA fixed mouse hippocampus neurons with an anti-MAP2 primary antibody (188 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 488 anti-msIgG1 sdAb (Cat. No. 2002, represented in red), an anti-GFAP primary antibody (173 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 647N anti-msIgG1 sdAb (Cat. No. 2002, represented in green) and an anti-Synapsin1 primary antibody (106 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 565 anti-msIgG1 sdAb (Cat. No. 2002, represented in yellow). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X2 anti-Mouse IgG1

Cat No: N2002 Category:

195,00 €

FluoTag®-X2 anti-Mouse IgG1 (Smart Secondaries®) is a species-specific FluoTag® directed against IgG1 from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications.

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In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.

Our anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags®, please check our Technology Section.

 

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μl N2002-At488-S AB_3076021
AZDye568 500 μl N2002-AF568-S AB_3076019
Atto643 500 μl N2002-At643-S AB_3076022
Alexa647 500 μl N2002-AF647-S AB_3076020
LI-COR IRDye 680RD 500 μl N2002-Li680-S AB_3076023
LI-COR IRDye 800CW 500 μl N2002-Li800-S AB_3076024
AbberiorStar635P 500 μl N2002-Ab635P-S AB_3076018
Related Products:

FluoTag®-X2 anti-Mouse IgG2a/b

FluoTag®-X2 anti-Rabbit IgG

FluoTag®-X2 anti-Chicken IgY
Clone: 10A4
Host: Alpaca
Produced in: E.coli
Application: IF, WB
Dilution: 1:500 (corresponding to 10 nM final concentration if the sdAb was reconstituted as proposed bellow)
Capacity: N/A
Antigen: -
Targets: Mouse IgG1
Specificity: Recognizes the native Mouse IgG1 subtype. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 5 µM.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Suggested general protocols for immunofluorescence can be found in our Resource Section.

One-Step immunofluorescence and multiplex staining can be performed with our smart secondaries. Here you can find more on these technologies One-Step-IF & Multiplexing.
References:
  1. Sanfilippo P, Kim AJ, Bhukel A, et al. Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome. Neuron. Published online January 13, 2024. doi:10.1016/j.neuron.2023.12.014 (IF; fruit fly)
  2. Haggerty KN, Eshelman SC, Sexton LA, et al. Super-resolution mapping in rod photoreceptors identifies rhodopsin trafficking through the inner segment plasma membrane as an essential subcellular pathway. PLoS Biol. 2024;22(1):e3002467. Published 2024 Jan 8. doi:10.1371/journal.pbio.3002467
  3. Queiroz Zetune Villa Real K, Mougios N, Rehm R, et al. A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy. Small Methods. 2023;7(10):e2300218. doi:10.1002/smtd.202300218 (ICC/IF; rat)
  4. Ring J, Tadic J, Ristic S, et al. The HSP40 chaperone Ydj1 drives amyloid beta 42 toxicity. EMBO Mol Med. 2022;14(5):e13952. doi:10.15252/emmm.202113952 (IHC; fruit fly)
  5. Agüi-Gonzalez P, Dankovich TM, Rizzoli SO, Phan NTN. Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry. Nanomaterials (Basel). 2021;11(7):1797. Published 2021 Jul 10. doi:10.3390/nano11071797 (ICC/IF; rat)
  6. Xiao C, Grzonka M, Meyer-Gerards C, Mack M, Figge R, Bazzi H. Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development. EMBO Rep. 2021;22(2):e51127. doi:10.15252/embr.202051127 (IHC; mouse)
  7. Saal KA, Warth Pérez Arias C, Roser AE, et al. Rho-kinase inhibition by fasudil modulates pre-synaptic vesicle dynamics. J Neurochem. 2021;157(4):1052-1068. doi:10.1111/jnc.15274 (IF, STED; rat primary neurons)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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