FluoTag®-X2 anti-Mouse IgG1

Cat No: N2002 Category:

170,00 

FluoTag®-X2 anti-Mouse IgG1 is a species-specific FluoTag® directed against IgG1 from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications

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In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.

Our anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.

 

MSDS
Variations:
Conjugation Amount Cat No.
AbberiorStar580 500 μl N2002-Ab580-S
AbberiorStar635P 500 μl N2002-Ab635P-S
Alexa647 500 μl N2002-AF647-S
Atto488 500 μl N2002-At488-S
AZDye568 500 μl N2002-AF568-S
LI-COR IRDye 680RD 500 μl N2002-Li680-S
LI-COR IRDye 800CW 500 μl N2002-Li800-S
Related Products:

FluoTag®-X2 anti-Mouse IgG2a/b

FluoTag®-X2 anti-Rabbit IgG

FluoTag®-X2 anti-Chicken IgY

Clone: 10A4
Host: Alpaca
Produced in: E.coli
Application: IF, WB
Dilution: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)
Capacity: N/A
Antigen: Mouse IgG1
Specificity: Recognizes the native Mouse IgG1 subtype. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4. Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Suggested general protocols for immunofluorescence can be found in our Resource Section.

One-Step immunofluorescence and multiplex staining can be performed with our smart secondaries. Here you can find more on these technologies One-Step-IF & Multiplexing

 

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.