FluoTag®-X2 anti-Mouse IgG1

Cat No: N2002 Category:

195,00 

FluoTag®-X2 anti-Mouse IgG1 (Smart Secondaries®) is a species-specific FluoTag® directed against IgG1 from Mouse. It serves as an effective secondary tool, enabling One-step immunofluorescence (IF) and species-independent Multiplexing.

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In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.

FluoTag®-X2 anti-Mouse IgG1 is a fluorescent single-domain antibody (sdAb) that binds specifically and strongly to mouse IgG1, independently of the IgGs light chains. As part of our Smart Secondaries® range, the FluoTag®-X2 anti-Mouse IgG1 is particularly suited for One-step immunofluorescence (One-step IF) and species-independent Multiplexing.

FluoTag®-X2 anti-Mouse IgG1 is site-specifically conjugated to two fluorophores per sdAb (FluoTag®–X2 variant). To learn more about FluoTags®, please visit our Technology section here.

We provide this product conjugated to various fluorophores and offer custom conjugation services with most commercially available fluorophores, provided these are available in a thiol-reactive activated form.

Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μl N2002-At488-S AB_3076021
AZDye568 500 μl N2002-AF568-S AB_3076019
Atto643 500 μl N2002-At643-S AB_3076022
Alexa647 500 μl N2002-AF647-S AB_3076020
LI-COR IRDye 680RD 500 μl N2002-Li680-S AB_3076023
LI-COR IRDye 800CW 500 μl N2002-Li800-S AB_3076024
AbberiorStar635P 500 μl N2002-Ab635P-S AB_3076018
Related Products:

FluoTag®-X2 anti-Mouse IgG2a/b

FluoTag®-X2 anti-Rabbit IgG

FluoTag®-X2 anti-Chicken IgY

Clone: 10A4
Host: Alpaca
Produced in: E.coli
Application:

IF (ICC), IHC, WB

Dilution: 1:500 (corresponding to 10 nM final concentration if the sdAb was reconstituted as proposed bellow)
Capacity: N/A
Antigen: -
Targets: Mouse IgG1
Specificity: Recognizes the native Mouse IgG1 subtype. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation:

The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50% glycerol in deionized water. We recommend including 0.1% sodium azide as a preservative if applicable. When reconstituted with 500 µL, the single-domain antibody concentration is 5 µM. For more details, click the Protocols button above and check Reconstitution and Storage.

kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.

Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.

References:
  1. Sanfilippo P, Kim AJ, Bhukel A, et al. Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome. Neuron. Published online January 13, 2024. doi:10.1016/j.neuron.2023.12.014 (IF; fruit fly)
  2. Haggerty KN, Eshelman SC, Sexton LA, et al. Super-resolution mapping in rod photoreceptors identifies rhodopsin trafficking through the inner segment plasma membrane as an essential subcellular pathway. PLoS Biol. 2024;22(1):e3002467. Published 2024 Jan 8. doi:10.1371/journal.pbio.3002467
  3. Queiroz Zetune Villa Real K, Mougios N, Rehm R, et al. A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy. Small Methods. 2023;7(10):e2300218. doi:10.1002/smtd.202300218 (ICC/IF; rat)
  4. Ring J, Tadic J, Ristic S, et al. The HSP40 chaperone Ydj1 drives amyloid beta 42 toxicity. EMBO Mol Med. 2022;14(5):e13952. doi:10.15252/emmm.202113952 (IHC; fruit fly)
  5. Agüi-Gonzalez P, Dankovich TM, Rizzoli SO, Phan NTN. Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry. Nanomaterials (Basel). 2021;11(7):1797. Published 2021 Jul 10. doi:10.3390/nano11071797 (ICC/IF; rat)
  6. Xiao C, Grzonka M, Meyer-Gerards C, Mack M, Figge R, Bazzi H. Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development. EMBO Rep. 2021;22(2):e51127. doi:10.15252/embr.202051127 (IHC; mouse)
  7. Saal KA, Warth Pérez Arias C, Roser AE, et al. Rho-kinase inhibition by fasudil modulates pre-synaptic vesicle dynamics. J Neurochem. 2021;157(4):1052-1068. doi:10.1111/jnc.15274 (IF, STED; rat primary neurons)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.