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Multiplexing Blocker Mouse

Cat No: K0102-50 Categories: ,

175,00 €

Protein mixture containing a blend of sdAbs recognizing Mouse immunoglobulin G (IgG) molecules. This reagent can be used in multiplexing applications for an optional blocking step.

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Multiplexing Blocker Mouse (MXB-M) is a highly purified protein mixture containing a blend of sdAbs recognizing Mouse immunoglobulin G (IgG) molecules. This reagent can be used in multiplexing applications for an optional blocking step, recommended for protocols that require extended incubation or washing steps.

Highly purified proteins lyophilized from PBS.

MSDS
Protocols
Reconstitution and Storage
Multiplexing (MX-IF)
Variations:
Conjugation Amount Cat No. RRID
- 550 µl K0102-50 AB_3668669
Related Products: View related products
Clone: -
Host: -
Produced in: E. coli
Application: IF
Dilution: If reconstituted in 550 µl as suggested, use 10 µL per µg of primary antibody in multiplexing experiments.
Capacity: -
Antigen: -
Targets: Mouse IgG
Specificity: Specific for mouse IgGs.
Formulation:

Dissolve the powder in 550 µL of sterile 50% glycerol (v/v) prepared with deionized water. Optionally, add 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature.
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

For blocking in multiplexing applications, use 10 µL per µg of primary antibody. For more details, click the Protocols button above and check Multiplexing (MX-IF) . For additional information, visit our Resources page.
References:
  1. Steen PR, Unterauer EM, Masullo LA, Kwon J, Perovic A, Jevdokimenko K, Opazo F, Fornasiero EF, Jungmann R. The DNA-PAINT palette: a comprehensive performance analysis of fluorescent dyes. Nat Methods. 2024 Sep;21(9):1755-1762. doi: 10.1038/s41592-024-02374-8. Epub 2024 Aug 7. PMID: 39112798; PMCID: PMC11399092. (ICC/IF; Six-plex; DNA-PAINT; rat primary hippocampal neurons)
  2. Mougios N, Cotroneo ER, Imse N, et al. NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals. Nat Commun. 2024;15(1):8771. Published 2024 Oct 10. doi:10.1038/s41467-024-53030-w
  3. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (DNA-PAINT)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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