Multiplexing: Indirect immunostaining of PFA fixed Cos7 cells with an anti-beta-Actin primary antibody (Cat. No. 251 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 565 anti-mskappaLC sdAb (Cat. No. 1202-At565-S, represented in red) and an anti-alpha-Tubulin primary antibody (Cat. No. 302 211, Synaptic Systems, dilutiuon 1:500) detected with FluoTag®-X2 Atto 488 anti-mskappaLC sdAb (Cat. No. 1202-At488-S, represented in green). Nuclei were visualized by DAPI staining (blue).
FluoTag®-X2 anti-Mouse Immunoglobulin kappa light chain is a species-specific FluoTag® directed against the kappa light chain from Mouse.
Most research monoclonal antibodies are generated in mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be further categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to the subclasses, each IgG molecule can be equipped with either a lambda, or kappa light chain.
Our sdAbs anti-mouse KLC specifically binds to all mouse Ig isotypes carrying a kappa light chain.
For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.
|Produced in:||E. coli|
|Antigen:||Mouse kappa light chain|
|Specificity:||Specifically recognizes Mouse Immunoglobulin kappa light chains. Does not cross-react with antibodies from species like: Rat, Guinea Pig, Rabbit, Chicken or Goat.|
|Formulation:||Lyophilized from PBS pH 7.4. Reconstitute with 500 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|