Indirect immunostaining of PFA fixed Hela cells with an anti-beta-Actin primary antibody (Cat. No. 251 006, dilution 1:500) detected with FluoTag®-X2 Alexa Fluor 568 anti-Chicken IgY sdAb (Cat. No. N0702-AF568-S, dilution 1:500, the FluoTag® signal is represented in red). Nuclei were visualized by DAPI staining (blue).
FluoTag®-X2 anti-Chicken is a species-specific FluoTag® directed against immunoglobulins from Chicken.
The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.
Our sdAbs anti-chicken IgY binds strongly and specifically to all chicken IgYs.
FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.
FluoTag®-X2 anti-Mouse IgG2a/b
|Dilution:||1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)|
|Formulation:||The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 5.0 µM.|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Suggested general protocols for immunofluorescence can be found in our Resource Section.
One-Step immunofluorescence and multiplex staining can be performed with our smart secondaries. Here you can find more on these technologies One-Step-IF & Multiplexing
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
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