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Multiplexing (see our Technologies): Indirect immunostaining of PFA fixed Cos7 cells with an anti-beta-Actin primary antibody (Cat. No. 251 011, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 565 anti-mouseKLC sdAb (Cat. No. 1202-At565-S, represented in red) and an anti-alpha-Tubulin primary antibody (Cat. No. 302 211, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 Atto 488 anti-mouseKLC sdAb (Cat. No. 1202-At488-S, represented in green). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X2 anti-Mouse Ig kappa light chain

Cat No: N1202 Category:

195,00 €

FluoTag®-X2 anti-Mouse Immunoglobulin kappa light chain is a species-specific FluoTag® directed against the kappa light chain from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications.

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In most research monoclonal antibodies are generated in mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be further categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to the subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.

Our sdAbs anti-mouse KLC specifically binds to all mouse Ig isotypes carrying a kappa light chain.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μl N1202-At488-S AB_2744594
AZDye568 500 μl N1202-AF568-S AB_3075956
Atto643 500 μl N1202-At643-S AB_3075959
Alexa647 500 μl N1202-AF647-S AB_3075957
LI-COR IRDye 680RD 500 μl N1202-Li680-S AB_3075960
LI-COR IRDye 800CW 500 μl N1202-Li800-S AB_3075961
AbberiorStar635P 500 μl N1202-Ab635P-S AB_2744593
Related Products:

FluoTag®-X2 anti-Mouse IgG1

FluoTag®-X2 anti-Mouse IgG2a/b

FluoTag®-X2 anti-Rabbit IgG
Clone: 1A23
Host: Alpaca
Produced in: E. coli
Application: IF, WB
Dilution: 1:500 (corresponding to 10 nM final concentration if the sdAb was reconstituted as proposed bellow).
Capacity: -
Antigen: -
Targets: Mouse kappa light chain
Specificity: Specifically recognizes Mouse Immunoglobulin kappa light chains. Does not cross-react with antibodies from species like Rat, Guinea Pig, Rabbit, Chicken or Goat.
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 500 µl of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the single-domain antibody concentration is 5 µM.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Suggested general protocols for immunofluorescence can be found in our Resource Section.

One-Step immunofluorescence and multiplex staining can be performed with our smart secondaries. Here you can find more on these technologies One-Step-IF & Multiplexing.
References:
  1. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF; STED; rat)
  2. Mougios N, Opazo F, Rizzoli SO, Reshetniak S. Trafficking proteins show limited differences in mobility across different postsynaptic spines. iScience. 2023;26(2):105971. Published 2023 Jan 13. doi:10.1016/j.isci.2023.105971 (ICC/IF; rat)
  3. Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO. Heat denaturation enables multicolor X10-STED microscopy. Sci Rep. 2023;13(1):5366. Published 2023 Apr 1. doi:10.1038/s41598-023-32524-5 (ICC/IF; rat)
  4. Hoffmann C, Rentsch J, Tsunoyama TA, et al. Synapsin condensation controls synaptic vesicle sequestering and dynamics. Nat Commun. 2023;14(1):6730. Published 2023 Oct 23. doi:10.1038/s41467-023-42372-6 
  5. Alvelid J, Damenti M, Sgattoni C, Testa I. Event-triggered STED imaging. Nat Methods. 2022;19(10):1268-1275. doi:10.1038/s41592-022-01588-y (ICC/IF; rat)
  6. Upmanyu N, Jin J, Emde HV, et al. Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3. Neuron. 2022;110(9):1483-1497.e7. doi:10.1016/j.neuron.2022.02.008
  7. Hodzic D, Wu J, Krchma K, et al. The inner nuclear membrane protein NEMP1 supports nuclear envelope openings and enucleation of erythroblasts. PLoS Biol. 2022;20(10):e3001811. Published 2022 Oct 10. doi:10.1371/journal.pbio.3001811 (IF; mouse)
  8. Reichert JS. SV2A-just a synaptic vesicle protein? Unravelling the interaction of SV2A and mitochondria in the pathogenesis and therapy of Morbus Alzheimer. PhD thesis (2022). University of Mainz. (SRM)
  9. Dankovich TM, Kaushik R, Olsthoorn LHM, et al. Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R. Nat Commun. 2021;12(1):7129. Published 2021 Dec 8. doi:10.1038/s41467-021-27462-7
  10. Mookherjee D, Das S, Mukherjee R, et al. RETREG1/FAM134B mediated autophagosomal degradation of AMFR/GP78 and OPA1 -a dual organellar turnover mechanism. Autophagy. 2021;17(7):1729-1752. doi:10.1080/15548627.2020.1783118 (ICC/IF; STED; tested cells: CHO)
  11. Maidorn M, Olichon A, Rizzoli SO, Opazo F. Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons. MAbs. 2019;11(2):305-321. doi:10.1080/19420862.2018.1551675
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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