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Indirect immunostaining of PFA fixed mouse brain section with Chicken anti-MAP2 (SYSY cat. no. 188 006) and sdAb anti-Chicken IgY conjugated to Sulfo-Cyanin 5 (green). Nuclei have been visualized by DAPI staining (blue).

sdAb anti-Chicken IgY

Cat No: N0705 Category:

275,00 € – 625,00 €

sdAb anti-Chicken IgY (Smart Secondaries®) is a single-domain anitbody against Chicken IgY that can be labeled or immobilized by the customer, making it an effective secondary tool for demanding immunolabeling and imaging applications.

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The immunorepertoire of chickens is much simpler compared to any other mammal, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.

sdAb anti-Chicken IgY binds strongly and specifically to Chicken IgYs.

To learn more about the Smart Secondaries®, please visit our Technology section here.

We provide this product conjugated to various labels and offer custom conjugation services with most commercially available labels, provided these are available in a thiol-reactive activated form.

 

MSDS
Protocols
Reconstitution and Storage
Maleimide labeling
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N0705-250ug AB_3075950
Biotin 250 μg N0705-Biotin AB_3075951
DBCO 250 μg N0705-DBCO AB_3075952
Related Products: View related products
Clone: 3G2
Host: Alpaca
Produced in: E. coli
Application: IF, WB
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Chicken IgY
Specificity: Recognizes specifically Chicken IgY. Does not cross-react with antibodies from species like: Mouse, Rabbit, Guinea Pig, Rat or Goat.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.
kDa: 14,9
Ext Coef: 14628
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.