sdAb anti-Rabbit IgG

Cat No: N2405 Category:

275,00 625,00 

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Like many other mammals, the immune repertoire of rabbits consists of several immunoglobulins (Ig) isotypes (IgA, IgG, IgE, and IgM). However, compared to other species, rabbits possess only one type of IgG carrying either a lambda or kappa light chain. Historically, rabbit antibodies have been a rich source of polyclonal antibodies, which were employed in various fields of life sciences. They are employed either directly from animal sera or as affinity-purified reagents.  In addition, advances in antibody engineering over the last decade enabled the generation of monoclonal rabbit antibodies. Independent of its clonality and irrespectively of its VH domain, our single domain antibodies (sdAbs, or Nanobodies) bind the constant domain (Fc) of any rabbit IgG specifically and with high affinity, making this secondary tool ideal for demanding imaging applications.

 

MSDS
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N2405-250ug AB_3076046
Biotin 250 μg N2405-Biotin AB_3076047
DBCO 250 μg N2405-DBCO AB_3076048
HRP 200 μl N2405-HRP AB_3076049
Related Products: -
Clone: 10E10
Host: Alpaca
Produced in: E.coli
Application: IF, IHC, WB
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Rabbit IgG
Specificity: Recognizes native Rabbit IgG. It does not cross-react with Mouse, Guinea Pig, Chicken, Rat, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation: 1)The DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Reconstitute with 250 µL 50% glycerol for storage at -80°C. 2)The unconjugated with a C-terminal Cys sdAbs are lyophilized from 10 mM KPi pH 6.0, 150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water. Use unconjugated sdAbs immediately after reconstitution. 3)The HRP-conjugated sdAbs are lyophilized from HRP-Protector. Reconstitute HRP-conjugated sdAbs with 200 µL pure water. Store at 4°C.
kDa: 14.5
Ext Coef: 22648
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for upto 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

Visit our Resource page for more protocols.

Here you find our suggestion for the conjugation of maleimide chemistry to our sdAbs cysteine.

References:
  1. Hellmeier J, Strauss S, Xu S, et al. Quantification of absolute labeling efficiency at the single-protein level. Nat Methods. Published online April 24, 2024. doi:10.1038/s41592-024-02242-5 (ICC/IF; DNA-PAINT)
  2. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF; DNA-PAINT)
  3. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (WB; tested species: mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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