sdAb anti-Rabbit IgG

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. The HRP-conjugated sdAbs are lyophilized from HRP protector buffer and should be reconstituted with 200 µL pure deionized water for storage at 4°C or with 200 µL high purity, sterile 50% glycerol for storage at -80°C. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks (Un-, DBCO-, Biotin-conjugated sdAbs) or at 4°C for up to 6 months (HRP-conjugated sdAbs). For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

Relevant protocols can be found under the “Protocols” button above. For additional information, visit our Resources page.

1. Hellmeier J, Strauss S, Xu S, et al. Quantification of absolute labeling efficiency at the single-protein level. Nat Methods. Published online April 24, 2024. doi:10.1038/s41592-024-02242-5 (ICC/IF; DNA-PAINT)
2. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF; DNA-PAINT)
3. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (WB; tested species: mouse)