Contact Shop

Indirect immunostaining of PFA fixed Cos7 cells with an anti-Aif rabbit polyclonal affinity-purified primary antibody (Cat. No. 300 003, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 AbberiorSTAR 580 anti-rabbit IgG sdAb (Cat. No. N2404-Ab580-S). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X4 anti-Rabbit IgG

Cat No: N2404 Category:

195,00 €

This new FluoTag®-X4 anti-Rabbit IgG is a species-specific FluoTag® directed against immunoglobulins from Rabbit. Especially useful for One-step immunofluorescence.

Read more

Like many other mammals, the immune repertoire of rabbits consists of several immunoglobulin (Ig) isotypes (IgA, IgG, IgE, and IgM). However, compared to other species, rabbits possess only one type of IgG carrying either a lambda or kappa light chain. Historically, rabbit antibodies have been a rich source of polyclonal antibodies, which were employed in various fields of life sciences. They are employed either directly from animal sera, or as affinity-purified reagents.  In addition, advances in antibody engineering over the last decade enabled the generation of monoclonal rabbit antibodies. Independent of its clonality and irrespectively of its VH domain, our single domain antibodies (sdAbs, or Nanobodies) bind the constant domain (Fc) of any rabbit IgG specifically and strongly, making this secondary tool ideal for demanding imaging applications.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags, please check our Technology Section.

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μl N2404-At488-S AB_2936445
AZDye568 500 μl N2404-AF568-S AB_3076042
Atto643 500 μl N2404-At643-S AB_3076045
Alexa647 500 μl N2404-AF647-S AB_3076043
AbberiorStar635P 500 μl N2404-Ab635P-S AB_3076041
Related Products:

FluoTag®-X2 anti-Mouse IgG1

FluoTag®-X2 anti-Mouse IgG2a/b

FluoTag®-X2 anti-Rabbit IgG
Clone: 10E10, 8H10
Host: Alpaca
Produced in: E.coli
Application: IF, WB
Dilution: 1:500 (corresponding to 5 nM final concentration for each clone if reconstituted as proposed bellow)
Capacity: N/A
Antigen: -
Targets: Rabbit IgG
Specificity: Recognizes native Rabbit IgG. It does not cross-react with Mouse, Guinea Pig, Chicken, Rat, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation: A mixture of two sdAb clones lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of each single-domain antibody clone is 2.5 µM.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Suggested general protocols for immunofluorescence can be found in our Resource Section.

One-Step immunofluorescence can be performed with our smart secondaries. Here you can find more on these technologies One-Step-IF.
References:
  1. Sanfilippo P, Kim AJ, Bhukel A, et al. Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome. Neuron. Published online January 13, 2024. doi:10.1016/j.neuron.2023.12.014 (IF; fruit fly)
  2. Yoo J, Dombrovski M, Mirshahidi P, et al. Brain wiring determinants uncovered by integrating connectomes and transcriptomes. Curr Biol. 2023;33(18):3998-4005.e6. doi:10.1016/j.cub.2023.08.020
  3. Sullenberger C, Kong D, Avazpour P, Luvsanjav D, Loncarek J. Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning. J Cell Biol. 2023;222(12):e202301092. doi:10.1083/jcb.202301092 (IF; HeLa)
  4. Hodzic D, Wu J, Krchma K, et al. The inner nuclear membrane protein NEMP1 supports nuclear envelope openings and enucleation of erythroblasts. PLoS Biol. 2022;20(10):e3001811. Published 2022 Oct 10. doi:10.1371/journal.pbio.3001811 (IF; mouse)
  5. Hausrat TJ, Vogl C, Neef J, et al. Monoallelic loss of the F-actin-binding protein radixin facilitates startle reactivity and pre-pulse inhibition in mice. Front Cell Dev Biol. 2022;10:987691. Published 2022 Nov 28. doi:10.3389/fcell.2022.987691 (IHC; mouse cochleae)
  6. Reichert JS. SV2A-just a synaptic vesicle protein? Unravelling the interaction of SV2A and mitochondria in the pathogenesis and therapy of Morbus Alzheimer. PhD thesis (2022). University of Mainz. (IHC, SRM; mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

Related products