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Indirect immunostaining of PFA fixed Cos7 cells with an anti-Aif rabbit polyclonal affinity-purified primary antibody (Cat. No. 300 003, Synaptic Systems, dilution 1:500) detected with FluoTag®-X2 AbberiorSTAR 580 anti-rabbit IgG sdAb (Cat. No. N2404-Ab580-S). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X4 anti-Rabbit IgG

Cat No: N2404 Category:

195,00 €

This new FluoTag®-X4 anti-Rabbit IgG (Smart Secondaries®) is a species-specific FluoTag® directed against immunoglobulins from Rabbit. It serves as an effective secondary tool, particularly useful for One-step immunofluorescence.

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FluoTag®-X4 anti-Rabbit IgG is a fluorescent single-domain antibody (sdAb, nanobody, VHH) with high specificity and affinity for Rabbit IgG. It binds the constant Fc domain of any Rabbit IgG, regardless of clonality or VH domain, making it an ideal secondary tool for demanding imaging applications. As part of our Smart Secondaries® range, the FluoTag®-X4 anti-Rabbit IgG is particularly suited for One-step immunofluorescence (One-step IF).

FluoTag®-X4 anti-Rabbit IgG is a blend of two different sdAbs, each carrying two fluorophores, that bind simultaneously to Rabbit IgG. We also offer our standard FluoTag®-X2 anti-Rabbit IgG, which is directly conjugated to two fluorophores per sdAb. To learn more about FluoTags®, please visit our Technology section here.

We provide this product conjugated to various fluorophores and offer custom conjugation services with most commercially available fluorophores, provided these are available in a thiol-reactive activated form.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μl N2404-At488-S AB_2936445
AZDye568 500 μl N2404-AF568-S AB_3076042
Atto643 500 μl N2404-At643-S AB_3076045
Alexa647 500 μl N2404-AF647-S AB_3076043
AbberiorStar635P 500 μl N2404-Ab635P-S AB_3076041
Related Products:

FluoTag®-X2 anti-Mouse IgG1

FluoTag®-X2 anti-Mouse IgG2a/b

FluoTag®-X2 anti-Rabbit IgG
Clone: 10E10, 8H10
Host: Alpaca
Produced in: E.coli
Application: IF, WB
Dilution: 1:500 (corresponding to 5 nM final concentration for each clone if reconstituted as proposed below).
Capacity: N/A
Antigen: -
Targets: Rabbit IgG
Specificity: Recognizes native Rabbit IgG. It does not cross-react with Mouse, Guinea Pig, Chicken, Rat, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation:

A mixture of two sdAb clones lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50% glycerol in deionized water. We recommend including 0.1% sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of each single-domain antibody clone is 2.5 µM. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the “Protocols” button above and check “Reconstitution and Storage”.

Protocols:

Relevant protocols can be found under the “Protocols” button above. For additional information, visit our Resources page.
References:
  1. Sanfilippo P, Kim AJ, Bhukel A, et al. Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome. Neuron. Published online January 13, 2024. doi:10.1016/j.neuron.2023.12.014 (IF; fruit fly)
  2. Yoo J, Dombrovski M, Mirshahidi P, et al. Brain wiring determinants uncovered by integrating connectomes and transcriptomes. Curr Biol. 2023;33(18):3998-4005.e6. doi:10.1016/j.cub.2023.08.020
  3. Sullenberger C, Kong D, Avazpour P, Luvsanjav D, Loncarek J. Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning. J Cell Biol. 2023;222(12):e202301092. doi:10.1083/jcb.202301092 (IF; HeLa)
  4. Hodzic D, Wu J, Krchma K, et al. The inner nuclear membrane protein NEMP1 supports nuclear envelope openings and enucleation of erythroblasts. PLoS Biol. 2022;20(10):e3001811. Published 2022 Oct 10. doi:10.1371/journal.pbio.3001811 (IF; mouse)
  5. Hausrat TJ, Vogl C, Neef J, et al. Monoallelic loss of the F-actin-binding protein radixin facilitates startle reactivity and pre-pulse inhibition in mice. Front Cell Dev Biol. 2022;10:987691. Published 2022 Nov 28. doi:10.3389/fcell.2022.987691 (IHC; mouse cochleae)
  6. Reichert JS. SV2A-just a synaptic vesicle protein? Unravelling the interaction of SV2A and mitochondria in the pathogenesis and therapy of Morbus Alzheimer. PhD thesis (2022). University of Mainz. (IHC, SRM; mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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