Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mNeonGreen reporter protein with FluoTag®-X2 Alexa Fluor 568 anti-mNeonGreen sdAb (Cat. No. N3202-AF568-L, dilution 1:500, the mNeonGreen signal is represented in green, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue).
FluoTag®-X2 anti-mNeonGreen is derived from an in-house developed single-domain antibody (sdAb) that recognizes mNeonGreen, which is a bright monomeric green fluorescent protein derived from Branchiostoma lanceolatume.
mNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.
Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor, have a look at our specificity chart in the resource section.
For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.
|Specificity:||Recognizes mNeonGreen. Does not cross-react with any GFP- or dsRed, of TagBFP derivatives.|
|Formulation:||Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
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