Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mNeonGreen reporter protein with FluoTag®-X2 Alexa Fluor 568 anti-mNeonGreen sdAb (Cat. No. N3202-AF568-L, dilution 1:500, the mNeonGreen signal is represented in green, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue).
FluoTag®-X2 anti-mNeonGreen is derived from an in-house developed single-domain antibody (sdAb) that recognizes mNeonGreen, which is a bright monomeric green fluorescent protein derived from Branchiostoma lanceolatume.
mNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.
An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”
Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor. Have a look at our specificity chart in our Resource Section.
FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.
|Dilution:||1:500 (corresponding to 5 nM final concentration)|
|Specificity:||Recognizes mNeonGreen. Does not cross-react with any GFP- or dsRed, of TagBFP derivatives.|
|Formulation:||The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Western Blotting is not recommended. The sdAbs tend to recognize native protein conformation only.
Look at detailed protocols and our specificity chart in our Resource Section.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|