Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mNeonGreen reporter protein with FluoTag®-X2 Alexa Fluor 568 anti-mNeonGreen sdAb (Cat. No. N3202-AF568-L, dilution 1:500, the mNeonGreen signal is represented in green, the corresponding FluoTag®-signal is represented in red and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue).

PFA-fixed Cos7 cells expressing a TOM70-nfmNeonGreen fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mNeonGreen coupled to Alexa Fluor 647 (Cat. No. N3202-AF647, dilution 1:500). A Greyscale image of the staining performed with N3202-AF647. B False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

PFA-fixed Cos7 cells expressing a TOM70- nfmNeonGreen fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mNeonGreen coupled to AZDye 568 (Cat. No. N3202-AF568, dilution 1:500). A Greyscale image of the staining performed with N3202-AF568. B False color representation of the image shown in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

PFA-fixed Cos7 cells expressing a TOM70- nfmNeonGreen fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mNeonGreen coupled to Atto 488 (Cat. No. N3202-At488, dilution 1:500). A Greyscale image of the staining performed with N3202-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.