375,00 €
FluoTag®-X2 anti-mNeonGreen is derived from an in-house developed single-domain antibody (sdAb) that recognizes mNeonGreen, which is a bright monomeric green fluorescent protein derived from Branchiostoma lanceolatume.
mNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.
Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor, have a look at our specificity chart in the resource section.
For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.
Variations: |
|
||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Related Products: | - | ||||||||||||||||||
Clone: | 1E2 | ||||||||||||||||||
Host: | Alpaca | ||||||||||||||||||
Produced in: | E.coli | ||||||||||||||||||
Application: | IF | ||||||||||||||||||
Dilution: | 1:500 | ||||||||||||||||||
Capacity: | - | ||||||||||||||||||
Antigen: | mNeonGreen | ||||||||||||||||||
Specificity: | Recognizes mNeonGreen. Does not cross-react with any GFP- or dsRed, of TagBFP derivatives. | ||||||||||||||||||
Formulation: | Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable. | ||||||||||||||||||
kDa: | - | ||||||||||||||||||
Ext Coef: | - | ||||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||||
Storing: | Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C. | ||||||||||||||||||
Protocols: |
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only. |
||||||||||||||||||
References: | - | ||||||||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |