Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mEos reporter protein with FluoTag®-X2 Abberior STAR 580 anti-mEos sdAb (Cat. No. N3102, dilution 1:500, the mEos signal is represented in green, the corresponding FluoTag-signal is represented in red and the merge of both channels is represented in yellow/orange). Nuclei were visualized by DAPI staining (blue).
FluoTag®-X2 anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.
In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several improved versions were discovered expanding the repertoir of photoswitchable fluorescent probes.
Our nanobody binds specifically and strongly to mEOS2, 3.2 & 4b; likely, it also binds other EOS fluorescent proteins. Have a look at our specificity chart in the resource section.
For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.
|Specificity:||Recognizes mEOS and mEOS derivatives.|
|Formulation:||Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
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