FluoTag®-X2 anti-mEos

Cat No: N3102 Category:

375,00 

FluoTag®-X2 anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.

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In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several improved versions were discovered expanding the repertoir of photoswitchable fluorescent probes.

Our nanobody binds specifically and strongly to mEOS2, 3.2 & 4b; likely, it also binds other EOS fluorescent proteins. Have a look at our specificity chart in the resource section.

For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.

 

 

 

MSDS
Variations:
Conjugation Amount Cat No.
AbberiorStar580 200 μl N3102-Ab580-L
AbberiorStar635P 200 μl N3102-Ab635P-L
Alexa647 200 μl N3102-AF647-L
Atto488 200 μl N3102-At488-L
AZDye568 200 μl N3102-AF568-L
Related Products: -
Clone: 1E8
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500
Capacity: N/A
Antigen: mEOS4b
Specificity: Recognizes mEOS and mEOS derivatives.
Formulation: Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.