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Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mEos reporter protein with FluoTag®-X2 Abberior STAR 580 anti-mEos sdAb (Cat. No. N3102, dilution 1:500, the mEos signal is represented in green, the corresponding FluoTag-signal is represented in red and the merge of both channels is represented in yellow/orange). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X2 anti-mEos

Cat No: N3102 Category:

375,00 €

FluoTag®-X2 anti-mEOS is derived from an in-house developed single-domain antibody (sdAb) that recognizes mEOS, which is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.

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In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several enhanced versions have been discovered expanding the repertoire of photoswitchable fluorescent probes. An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”

Our nanobody binds specifically and strongly to mEOS2, 3.2 & 4b; likely, it also binds other EOS fluorescent proteins. Have a look at our specificity chart in our Resource Section.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

 

 

 

MSDS Protocols
Variations:
Amount Conjugation Cat No.
Atto488 200 μl N3102-At488-L
AZDye568 200 μl N3102-AF568-L
Atto643 200 μl N3102-At643-L
Alexa647 200 μl N3102-AF647-L
AbberiorStar635P 200 μl N3102-Ab635P-L
Related Products: -
Clone: 1E8
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: mEOS4b
Specificity: Recognizes mEOS and mEOS derivatives.
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

This Product is not recommended to be used to detect proteins in Western Blott, sdAbs tend to recognize native/folded protein mainly.

Look at detailed protocols and our specificity chart in our Resource Section.

 

 
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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