Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mEOS reporter protein with FluoTag®-X2 Abberior STAR 580 anti-mEos sdAb (Cat. No. N3102, dilution 1:500, the mEos signal is represented in green, the corresponding FluoTag-signal is represented in red and the merge of both channels is represented in yellow/orange). Nuclei were visualized by DAPI staining (blue).

Confocal images of immunostained spinal cord sections from homozygous knock-in mEos4b-GlyRβ mice (top row, see [Maynard et al., 2021, eLife]) and wild-type animals that do not express mEos4b-tagged receptors (bottom row). The left panel displays the fluorescent signal of endogenous mEos4b-GlyRβ protein (green) that localises at inhibitory synapses. The middle panel shows the labeling of endogenous mEos4b-GlyRβ with FluoTag® anti-mEos-AF647 (Cat. No. N3102-AF647-L, dilution 1:1000, cyan). The right panel shows Gephyrin labeling as a positive control to mark inhibitory synapses using the mAb7a antibody (Synaptic Systems Cat. No.147011, dilution 1:1000, red) and a CF568-conjugated secondary antibody. Image courtesy of S. Camuso and C.G. Specht, Inserm, UPSaclay, Le Kremlin-Bicêtre, France [unpublished data].

dSTORM image of spinal cord section from knock-in mEos4b-GlyRβ mouse [Maynard et al., 2021, eLife]. mEos4b-tagged GlyRβ were labeled with FluoTag®-X2 anti-mEos coupled to Janelia Fluor® 635b (Cat. No. N3102-JF535B-L). Image courtesy of C.G. Specht and S. Camuso, Inserm, UPSaclay, Le Kremlin-Bicêtre, France.

PFA-fixed Cos7 cells expressing a TOM70-nfmEOS fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mEOS coupled to Alexa Fluor 647 (Cat. No. N3102-AF647, dilution 1:500). A Greyscale image of the staining performed with N3102-AF647. A typical mitochondrial staining was observed. B False color representation of the anti-mEOS signal depicted in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of all channels. False color representation of the DAPI signal: blue; the mEOS signal: magenta.

PFA-fixed Cos7 cells expressing a TOM70-nfmEOS fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mEOS coupled to AZDye 568 (Cat. No. N3102-AF568, dilution 1:500). A Greyscale image of the staining performed with N3102-AF568. A typical mitochondrial staining was observed.  B False color representation of the anti-mEOS signal depicted in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of all channels. False color representation of the DAPI signal: blue; the mEOS signal: red.

PFA-fixed Cos7 cells expressing a TOM70-nfmEOS fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mEOS coupled to Atto 488 (Cat. No. N3102-At488, dilution 1:500). A Greyscale image of the staining performed with N3102-At488. A typical mitochondrial staining was observed. B False color representation of the anti-mEOS signal depicted in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of all channels. False color representation of the DAPI signal: blue; the mEOS signal: green.