400,00 €
FluoTag®-X2 anti-mEOS is a fluorophore-conjugated single-domain antibody (sdAb) that recognizes mEOS – a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii. FluoTag®-X2 anti-mEOS enables direct and highly specific fluorescent detection of mEOS without the need for secondary antibodies.
In 2004 Wiedernmann and colleagues described an engineered fluorescent protein with photoswitchable properties, EosFP, which could be selectively shifted from green to red emission and was derived from a fluorescent protein discovered in Lobopyllia hemprichii. The same study also describes a monomeric, improved version, mEOS. Over the last years, several enhanced versions have been discovered expanding the repertoire of photoswitchable fluorescent probes. An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”.
Our nanobody binds specifically and strongly to mEOS2, 3.2 and 4b; likely, it also binds other EOS fluorescent proteins. Have a look at our FP Specificity Chart.
FluoTag®-X2 anti-mEOS is directly conjugated to two fluorophores per sdAb (FluoTag®–X2 variant). To learn more about the FluoTags®, please visit our Technology section here.
Variations: |
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Related Products: |
sdAb anti-mEos (Cat. No. N3105) View related products |
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Clone: | 1E8 | ||||||||||||||||||||||||||||
Host: | Alpaca | ||||||||||||||||||||||||||||
Produced in: | E.coli | ||||||||||||||||||||||||||||
Application: |
IF (ICC), IHC Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly. |
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Dilution: | 1:500 (corresponding to 5 nM final concentration) | ||||||||||||||||||||||||||||
Capacity: | N/A | ||||||||||||||||||||||||||||
Antigen: | - | ||||||||||||||||||||||||||||
Targets: | mEOS4b | ||||||||||||||||||||||||||||
Specificity: | Recognizes mEOS and mEOS derivatives. | ||||||||||||||||||||||||||||
Formulation: |
The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). For more details, click the Protocols button above and check Reconstitution and Storage. |
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kDa: | - | ||||||||||||||||||||||||||||
Ext Coef: | - | ||||||||||||||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
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Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page and review the FP Specificity Chart. |
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References: |
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Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |