Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mEOS reporter protein with FluoTag®-X2 Abberior STAR 580 anti-mEos sdAb (Cat. No. N3102, dilution 1:500, the mEos signal is represented in green, the corresponding FluoTag-signal is represented in red and the merge of both channels is represented in yellow/orange). Nuclei were visualized by DAPI staining (blue).

PFA-fixed Cos7 cells expressing a TOM70-nfmEOS fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mEOS coupled to Alexa Fluor 647 (Cat. No. N3102-AF647, dilution 1:500). A Greyscale image of the staining performed with N3102-AF647. A typical mitochondrial staining was observed. B False color representation of the anti-mEOS signal depicted in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of all channels. False color representation of the DAPI signal: blue; the mEOS signal: magenta.

PFA-fixed Cos7 cells expressing a TOM70-nfmEOS fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mEOS coupled to AZDye 568 (Cat. No. N3102-AF568, dilution 1:500). A Greyscale image of the staining performed with N3102-AF568. A typical mitochondrial staining was observed.  B False color representation of the anti-mEOS signal depicted in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of all channels. False color representation of the DAPI signal: blue; the mEOS signal: red.

PFA-fixed Cos7 cells expressing a TOM70-nfmEOS fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mEOS coupled to Atto 488 (Cat. No. N3102-At488, dilution 1:500). A Greyscale image of the staining performed with N3102-At488. A typical mitochondrial staining was observed. B False color representation of the anti-mEOS signal depicted in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of all channels. False color representation of the DAPI signal: blue; the mEOS signal: green.