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Immunostaining of PFA fixed Cos7 cells expressing a TOM70-mRuby reporter protein with FluoTag®-X2 Alexa Fluor 647 anti-mRuby sdAb (Cat. No. N3302-AF647-L, dilution 1:500, the mRuby signal is represented in red, the corresponding FluoTag-signal is represented in green and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X2 anti-mRuby

Cat No: N3302 Category:

375,00 €

FluoTag®-X2 anti-mRUBY is derived from an in-house developed single-domain antibody (sdAb) that recognizes mRuby and mRuby derivatives, which is a bright monomeric red fluorescent protein derived from Entacmaea quadricolar.

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mRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor . mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.

Our nanobody binds specifically and strongly to mRuby fluorescent proteins. Have a look at our specificity chart in the resource section.

For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.

 

MSDS
Variations:
Conjugation Amount Cat No.
AbberiorStar580 200 μl N3302-Ab580-L
AbberiorStar635P 200 μl N3302-Ab635P-L
Alexa647 200 μl N3302-AF647-L
Atto488 200 μl N3302-At488-L
AZDye568 200 μl N3302-AF568-L
Related Products: -
Clone: 1F8
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500
Capacity: N/A
Antigen: mRUBY3
Specificity: Recognizes most common mRuby derivatives
Formulation: Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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