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Indirect immunostaining of PFA fixed Hela cells with an anti-beta-Actin primary antibody (Cat. No. 251 006, dilution 1:500) detected with FluoTag®-X2 Alexa Fluor 568 anti-Chicken IgY sdAb (Cat. No. N0702-AF568-S, dilution 1:500, the FluoTag® signal is represented in red). Nuclei were visualized by DAPI staining (blue).

FluoTag®-X2 anti-Chicken IgY

Cat No: N0702 Category:

195,00 €

FluoTag®-X2 anti-Chicken IgY (Smart Secondaries®) is a species-specific FluoTag® directed against immunoglobulins from Chicken. This fluorescent secondary reagent enables efficient detection of primary antibodies raised in chicken, offering reliable performance in various applications.

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The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.

FluoTag®-X2 anti-Chicken IgY is a fluorescent single-domain antibody (sdAb) that binds strongly and specifically to all chicken IgYs. As part of our Smart Secondaries® range, the FluoTag®-X2 anti-Chicken IgY is particularly suited for One-step immunofluorescence (One-step IF) and species-independent Multiplexing.

FluoTag®-X2 anti-Chicken IgY is site-specifically conjugated to two fluorophores per sdAb (FluoTag®–X2 variant). To learn more about FluoTags®, please visit our Technology section here.

We provide this product conjugated to various fluorophores and offer custom conjugation services with most commercially available fluorophores, provided these are available in a thiol-reactive activated form.

MSDS
Protocols
Reconstitution and Storage
Multiplexing (MX-IF)
Variations:
Conjugation Amount Cat No. RRID
Atto488 500 μl N0702-At488-S AB_2744580
AZDye568 500 μl N0702-AF568-S AB_3075944
Atto643 500 μl N0702-At643-S AB_3075947
Alexa647 500 μl N0702-AF647-S AB_3075945
AbberiorStar635P 500 μl N0702-Ab635P-S AB_2744579
LI-COR IRDye 680RD 500 μl N0702-Li680-S AB_3075948
LI-COR IRDye 800CW 500 μl N0702-Li800-S AB_3075949
Related Products:

FluoTag®-X2 anti-Mouse IgG1

FluoTag®-X2 anti-Mouse IgG2a/b

FluoTag®-X2 anti-Rabbit IgG

 

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Clone: 3G2
Host: Alpaca
Produced in: E.coli
Application: IF, WB
Dilution: 1:500 (corresponding to 10 nM final concentration if the sdAb was reconstituted as proposed bellow)
Capacity: N/A
Antigen: -
Targets: Chicken IgY
Specificity: Chicken IgY
Formulation:

The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 500 µL of 50% glycerol in deionized water. We recommend including 0.1% sodium azide as a preservative if applicable. When reconstituted with 500 µL, the single-domain antibody concentration is 5 µM. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Levic DS, Ryan S, Marjoram L, Honeycutt J, Bagwell J, Bagnat M. Distinct roles for luminal acidification in apical protein sorting and trafficking in zebrafish. J Cell Biol. 2020;219(4):e201908225. doi:10.1083/jcb.201908225
  2. Jähne S. A quantitative analysis of an average cultured neuron. PhD thesis (2019). University of Göttingen.
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product, you agree to our general terms and conditions.