Multiplexing Blocker Rabbit

Cat No: K0202-50 Categories: ,

175,00 

Protein mixture containing a blend of sdAbs recognizing Rabbit immunoglobulin G (IgG) molecules. This reagent can be used in multiplexing applications for an optional blocking step.

 

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Multiplexing Blocker Rabbit (MXB-R) is a highly purified protein mixture containing a blend of sdAbs recognizing Rabbit immunoglobulin G (IgG) molecules. This reagent can be used in multiplexing applications for an optional blocking step, recommended for protocols that require extended incubation or washing steps.

Highly purified proteins lyophilized from PBS.

Variations:
Conjugation Amount Cat No. RRID
- 550 µl K0202-50 AB_3075894
Related Products: -
Clone: -
Host: -
Produced in: E. coli
Application: IF
Dilution: If reconstituted in 550 µl as suggested, use 10 µL per µg of primary antibody in multiplexing experiments.
Capacity: -
Antigen: -
Targets: Rabbit IgG
Specificity: Specific for rabbit IgG.
Formulation:

Dissolve the powder in 550 µL of sterile 50% glycerol (v/v) prepared with deionized water. Optionally, add 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.

kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.

Protocols:

For blocking in multiplexing applications, use 10 µL per µg of primary antibody. For more details, click the Protocols button above and check Multiplexing (MX-IF) . For additional information, visit our Resources page.

References:
  1. Steen PR, Unterauer EM, Masullo LA, Kwon J, Perovic A, Jevdokimenko K, Opazo F, Fornasiero EF, Jungmann R. The DNA-PAINT palette: a comprehensive performance analysis of fluorescent dyes. Nat Methods. 2024 Sep;21(9):1755-1762. doi: 10.1038/s41592-024-02374-8. Epub 2024 Aug 7. PMID: 39112798; PMCID: PMC11399092. (ICC/IF; Six-plex; DNA-PAINT; rat primary hippocampal neurons)
  2. Mougios N, Cotroneo ER, Imse N, et al. NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals. Nat Commun. 2024;15(1):8771. Published 2024 Oct 10. doi:10.1038/s41467-024-53030-w
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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