Rabbit anti-ALFA polyclonal serum

Lyophilized from 200 µL rabbit serum. Reconstitute with 200 µL 50% glycerol (v/v) in deionized water. If applicable, we recommend including 0.1% sodium azide as a preservative. For more details, click the Protocols button above and check Reconstitution and Storage.

Vials containing lyophilized antibodies can be stored at 2-8°C for up to 12 months. After reconstitution, working aliquots can be stored at 4°C for up to 4 weeks. For long term storage (up to 6 months), store reconstituted antibodies at -20°C or below. For more details, click the Protocols button above and check Reconstitution and Storage.

1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
2. Cheetham-Wilkinson IJ, Sivalingam B, Flitton C, et al. RpH-ILV: Probe for lysosomal pH and acute LLOMe-induced membrane permeabilization in cell lines and Drosophila. Sci Adv. 2025;11(1):eadr7325. doi:10.1126/sciadv.adr7325 (Immunogold labeling and electron microscopy; HEK293T)

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