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Ubiquitin K48 Selector

Cat No: N1810 Category:

275,00 €

Ubiquitin K48 Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

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Proteins in cells can be ubiquitinated where E3 ligase catalyzes the last step of this process. After conjugating the initial ubiquitin on the target protein, further ubiquitin proteins can be conjugated to the first ubiquitin resulting in a polyubiquitin chain. The conjugation of new ubiquitins to form a poly-chain happens at one of the seven lysines (K) present in ubiquitin. The addition of a new ubiquitin on the lysine at position 48 (K48) is a signal for the target protein to be sent to the proteasome for degradation.

Our sdAb binds most specifically to this K48 linkage on polyubiquitin chains.

This product was a co-development with

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
4% cross-linked agarose 2000 μl N1810 AB_3076013
Related Products: -
Clone: A4-1
Host: Synthetic Library
Produced in: E.coli
Application: IP
Dilution: N/A
Capacity: > 2.5 µg K-48 linked Ubiquitin per µL of packed beads
Antigen: -
Targets: Ubiquitin K48 linkage
Specificity: Recognizes K48-linkages of polyubiquitin.
Formulation: 50% slurry in PBS containing 20% Ethanol
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Store at 4 °C for up to 12 months. Do not freeze!
Protocols: -
References:
  1. Gonzalez-Santamarta M, Ceccato L, Carvalho AS, Rain JC, Matthiesen R, Rodriguez MS. Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome Using Chain-Specific Nanobodies. Methods Mol Biol. 2023;2602:125-136. doi:10.1007/978-1-0716-2859-1_9 (Isolation of ubiquitylated proteins, MS; MCL cells)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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