Contact Shop

Immunostaining of PFA fixed rat hippocampus neurons with FluoTag®-X2 anti-Synaptotagmin-1 AF568 (Cat. No. N4302-AZ568-L, dilution 1:500). The confocal image is shown on the left side and the corresponding STED image is shown on the right side.

sdAb anti-Synaptotagmin 1 (A51)

Cat No: N4305 Category:

Price range: 290,00 € through 655,00 €

sdAb anti-Synaptotagmin 1 (A51) is an alpaca single-domain antibody (sdAb) that recognizes specifically the cytoplasmic domain of rat and mouse Synaptotagmin 1 (SYT1) with high affinity and specificity.

Read more

Synaptotagmin 1 (SYT1) belongs to a family with 17 members. Synaptotagmins are all single-pass transmembrane proteins containing two domains binding to calcium at their intracellular portion.  Synaptotagmin 1 is enriched at the pre-synaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase of calcium concentration.

Our nanobody strongly and specifically binds to the cytosolic portion of mouse and rat SYT1.

 

MSDS
sdAb unconjugated
sdAb-Biotin/DBCO
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
Biotin 250 ÎĽg N4305-Biotin AB_3668682
DBCO 250 ÎĽg N4305-DBCO AB_3668683
unconjugated C-terminal Cys 250 ÎĽg N4305-250ug AB_3668681
Related Products:

FluoTag®-X2 anti-Synaptotagmin 1 (A51)

View related products
Clone: A51
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Synaptotagmin 1 (cytosolic)
Specificity: Recognizes the cytosolic domain of rat and mouse Synaptotagmin 1 (other species not tested).
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 15,3
Ext Coef: 25628
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Queiroz Zetune Villa Real K, Mougios N, Rehm R, et al. A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy. Small Methods. 2023;7(10):e2300218. doi:10.1002/smtd.202300218
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms:

By purchasing this product you agree to our general terms and conditions.