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Immunostaining of PFA fixed rat hippocampus neurons with FluoTag®-X2 anti-Synaptotagmin-1 AF568 (Cat. No. N4302-AZ568-L, dilution 1:500). The confocal image is shown on the left side and the corresponding STED image is shown on the right side.

sdAb anti-Synaptotagmin 1 (A51)

Cat No: N4305 Category:

275,00 € – 625,00 €

FluoTag®-X2 anti-Synaptotagmin 1 A51 (SYT1) is an alpaca single-domain antibody (sdAb) that recognizes specifically the cytoplasmic domain of murine Synaptotagmin 1 with high affinity and specificity.

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Synaptotagmin 1 (Syt1) belongs to a family with 17 members. Synaptotagmins are all single-pass transmembrane proteins containing two domains binding to calcium at their intracellular portion.  Synaptotagmin 1 is enriched at the pre-synaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase of calcium concentration.

Our nanobody recognizes strongly and specifically the cytosolic portion of Syt1.

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
Biotin 250 μg N4305-Biotin -
DBCO 250 μg N4305-DBCO -
unconjugated C-terminal Cys 250 μg N4305-250ug -
Related Products: -
Clone: A51
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Synaptotagmin 1 (cytosolic)
Specificity: Recognizes the cytosolic domain of rat and mouse Synaptotagmin 1 (other species not tested).
Formulation: DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconstitute with 250 µL pure water (unconjugated sdAbs) or with 250 µL 50% glycerol for storage at -80°C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution.
kDa: 15,3
Ext Coef: 25628
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

You can find protocols on our Resources page
References:
  1. Queiroz Zetune Villa Real K, Mougios N, Rehm R, et al. A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy. Small Methods. 2023;7(10):e2300218. doi:10.1002/smtd.202300218
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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