FluoTag®-X2 anti-VGlut1

Cat No: N1602 Category:

400,00 

FluoTag®-X2 anti-VGLUT1 is derived from a single-domain antibody (sdAb) recognizing specifically VGLUT1 with high affinity and specificity.

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The vesicula glutamate transport 1 (vGlut1) is primarily located on the synaptic vesicles of glutamatergic synapses (excitatory). Its function is to fill the vesicles with the neurotransmitter glutamate before they fuse to the plasma membrane and release their content from the pre-synaptic compartment into the synaptic cleft to stimulate postsynaptic receptors. vGlut1 uses the energy from the proton gradient generated by the vesicular ATPase to transport glutamate inside the vesicle.

Our sdAb binds selectively and strongly to a cytoplasmic portion of the murine vGlut1.

FluoTags are directly conjugated to fluorophores, however, they can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores, decorating the target protein with 4 fluorophores in total (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μl N1602-At488-L AB_3076005
AZDye568 200 μl N1602-AF568-L AB_3076003
Atto643 200 μl N1602-At643-L AB_3076006
Alexa647 200 μl N1602-AF647-L AB_3076004
AbberiorStar635P 200 μl N1602-Ab635P-L AB_2737072
Related Products: -
Clone: Nb9
Host: Synthetic Library
Produced in: E.coli
Application: IF
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: vGlut1
Specificity: Recognizes the cytosolic domain of rat vGlut1 (other species not tested).
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

This Product is not recommended to be used to detect proteins in Western Blott, sdAbs tend to recognize native/folded protein mainly.

Look at detailed protocols in our Resource Section.

References:
  1. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF, STED, DNA-PAINT; rat and IHC/IF, DNA-PAINT; mouse)
  2. Falkovich R, Danielson EW, Perez de Arce K, et al. A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging. Cell Rep. 2023;42(5):112430. doi:10.1016/j.celrep.2023.112430 (IF; rat hippocampal neurons)
  3. Reichert JS. SV2A-just a synaptic vesicle protein? Unravelling the interaction of SV2A and mitochondria in the pathogenesis and therapy of Morbus Alzheimer. PhD thesis (2022). University of Mainz. (IHC; mouse)
  4. Dankovich TM, Kaushik R, Olsthoorn LHM, et al. Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R. Nat Commun. 2021;12(1):7129. Published 2021 Dec 8. doi:10.1038/s41467-021-27462-7 (ICC/IF, STED; mouse)
  5. Agüi-Gonzalez P, Dankovich TM, Rizzoli SO, Phan NTN. Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry. Nanomaterials (Basel). 2021;11(7):1797. Published 2021 Jul 10. doi:10.3390/nano11071797 (ICC/IF; rat)
  6. Perego E, Reshetniak S, Lorenz C, et al. A minimalist model to measure interactions between proteins and synaptic vesicles. Sci Rep. 2020;10(1):21086. Published 2020 Dec 3. doi:10.1038/s41598-020-77887-1 (IF; synaptic vesicles from rat brain)
  7. Gürth CM, Dankovich TM, Rizzoli SO, D’Este E. Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway. Sci Rep. 2020;10(1):20576. Published 2020 Nov 25. doi:10.1038/s41598-020-77260-2 (IF, STED; rat hippocampal primary neurons)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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