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Direct immunofluorescence labeling of a cultured hippocampus neuron (1:500). Nerve-terminals positive for VGLUT1 were visualized with FluoTag-X2 anti-VGLUT1 AbberiorSTAR® 580 (red). Counterstaining with rabbit anti-MAP2 Cy5 (false color representation in green) and dapi (blue).

FluoTag®-X2 anti-VGlut1

Cat No: N1602 Category:

375,00 €

FluoTag®-X2 anti-VGLUT1 is derived from a single-domain antibody (sdAb) recognizing specifically VGLUT1 with high affinity and specificity.

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The vesicula glutamate transport 1 (vGlut1) is primarily located on the synaptic vesicles of glutamatergic synapses (excitatory). Its function is to fill the vesicles with the neurotransmitter glutamate before they fuse to the plasma membrane and release their content from the pre-synaptic compartment into the synaptic cleft to stimulate postsynaptic receptors. vGlut1 uses the energy from the proton gradient generated by the vesicular ATPase to transport glutamate inside the vesicle.

Our sdAb binds selectively and strongly to a cytoplasmic portion of the murine vGlut1.

FluoTags are directly conjugated to fluorophores, however, they can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores, decorating the target protein with 4 fluorophores in total (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS
Variations:
Conjugation Amount Cat No.
Atto488 200 μl N1602-At488-L
AZDye568 200 μl N1602-AF568-L
Atto643 200 μl N1602-At643-L
Alexa647 200 μl N1602-AF647-L
AbberiorStar635P 200 μl N1602-Ab635P-L
Related Products: -
Clone: Nb9
Host: Synthetic Library
Produced in: E.coli
Application: IF
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: vGlut1
Specificity: Recognizes the cytosolic domain of rat vGlut1 (other species not tested).
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

This Product is not recommended to be used to detect proteins in Western Blott, sdAbs tend to recognize native/folded protein mainly.

Look at detailed protocols in our Resource Section.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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