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Direct immunofluorescence labeling of a cultured hippocampus neurons. Nerve-terminals positive for VGLUT1 were visualized with FluoTag®-X2 anti-VGLUT1 AbberiorSTAR® 580 (dilution 1:500; red). Counterstaining was performed with rabbit anti-MAP2 Cy5 (green). Nuclei were visulized with DAPI (blue).

FluoTag®-X2 anti-VGLUT1

Cat No: N1602 Category:

400,00 €

A fluorophore-conjugated single-domain antibody that specifically and strongly binds to the VGLUT1.

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FluoTag®-X2 anti-VGLUT1 is derived from an in-house developed single-domain antibody (sdAb) that specifically recognizes a vesicular glutamate transporter 1 (VGLUT1) protein. VGLUT1 is primarily located on the synaptic vesicles of glutamatergic synapses (excitatory). Its function is to fill the vesicles with the neurotransmitter glutamate before they fuse to the plasma membrane and release their content from the pre-synaptic compartment into the synaptic cleft to stimulate postsynaptic receptors. VGLUT1 harnesses the energy from the proton gradient, which is generated by the vesicular ATPase, to transport glutamate into the vesicle.

Our sdAb binds selectively and strongly to a cytoplasmic portion of the murine VGLUT1.

FluoTag®-X2 anti-VGLUT1 is directly conjugated to two fluorophores per sdAb (FluoTag®-X2 variant). To learn more about the FluoTags®, please visit our Technology section here.

MSDS
Protocols
Reconstitution and Storage
IF (ICC) Protocol
Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μl N1602-At488-L AB_3076005
AZDye568 200 μl N1602-AF568-L AB_3076003
Atto643 200 μl N1602-At643-L AB_3076006
Alexa647 200 μl N1602-AF647-L AB_3076004
AbberiorStar635P 200 μl N1602-Ab635P-L AB_2737072
Related Products: -
Clone: Nb9
Host: Llama immune library
Produced in: E.coli
Application:

IF

Note: This Product is not recommended for detecting proteins in Western Blot, as sdAbs tend to recognize mainly native or folded proteins.
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: vGlut1
Specificity: Recognizes the cytosolic domain of rat VGLUT1. Tested on rat tissue. Based on high sequence similarity, should also recognize mouse and human VGLUT1.
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). For more details, click the "Protocols" button above and check "Reconstitution and Storage".
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the "Protocols" button above and check "Reconstitution and Storage".
Protocols:

Relevant protocols can be found under the “Protocols” button above. For additional information, visit our Resources page.
References:
  1. Unterauer EM, Shetab Boushehri S, Jevdokimenko K, et al. Spatial proteomics in neurons at single-protein resolution. Cell. 2024;187(7):1785-1800.e16. doi:10.1016/j.cell.2024.02.045 (ICC/IF, STED, DNA-PAINT; rat and IHC/IF, DNA-PAINT; mouse)
  2. Falkovich R, Danielson EW, Perez de Arce K, et al. A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging. Cell Rep. 2023;42(5):112430. doi:10.1016/j.celrep.2023.112430 (IF; rat hippocampal neurons)
  3. Reichert JS. SV2A-just a synaptic vesicle protein? Unravelling the interaction of SV2A and mitochondria in the pathogenesis and therapy of Morbus Alzheimer. PhD thesis (2022). University of Mainz. (IHC; mouse)
  4. Dankovich TM, Kaushik R, Olsthoorn LHM, et al. Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R. Nat Commun. 2021;12(1):7129. Published 2021 Dec 8. doi:10.1038/s41467-021-27462-7 (ICC/IF, STED; mouse)
  5. Agüi-Gonzalez P, Dankovich TM, Rizzoli SO, Phan NTN. Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry. Nanomaterials (Basel). 2021;11(7):1797. Published 2021 Jul 10. doi:10.3390/nano11071797 (ICC/IF; rat)
  6. Perego E, Reshetniak S, Lorenz C, et al. A minimalist model to measure interactions between proteins and synaptic vesicles. Sci Rep. 2020;10(1):21086. Published 2020 Dec 3. doi:10.1038/s41598-020-77887-1 (IF; synaptic vesicles from rat brain)
  7. Gürth CM, Dankovich TM, Rizzoli SO, D’Este E. Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway. Sci Rep. 2020;10(1):20576. Published 2020 Nov 25. doi:10.1038/s41598-020-77260-2 (IF, STED; rat hippocampal primary neurons)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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