Immunostaining of PFA fixed mouse hippocampus neurons cultures with FluoTag®-X2 anti-GFAP AlexaFluor 568 (Cat. No. N3802-AF568-L, dilution 1:500, represented in red). Nuclei were visualized by DAPI staining (blue).

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-GFAP coupled to Alexa Fluor 647 (Cat. No. N3802-AF647, dilution 1:500). A Greyscale image of the staining performed with the anti-GFAP single-domain antibody N3802-AF647. B Greyscale image of the counterstaining performed with an anti-VGlut1 single-domain antibody N1602-At488. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in magenta, B in green and C in blue.

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-GFAP coupled to AZDye 568 (Cat. No. N3802-AF568, dilution 1:500). A Greyscale image of the staining performed with the anti-GFAP single-domain antibody N3802-AF568. B Greyscale image of the counterstaining performed with an anti-VGlut1 single-domain antibody N1602-At488. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in red, B in green and C in blue.

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-GFAP coupled to Atto 488 (Cat. No. N3802-At488, dilution 1:500). A Greyscale image of the staining performed with the anti-GFAP single-domain antibody N3802-At488. B Greyscale image of the counterstaining performed with an anti-PSD95 single-domain antibody N3702-AF647. C DAPI signal of the depicted section. D Merge of A – C. False color representation of A in green, B in magenta and C in blue.