Immunostaining of PFA fixed mouse hippocampus neurons cultures with FluoTag®-X2 AlexaFluor 568 anti-GFAP sdAb (Cat. No. N3802-AF568-L, dilution 1:500, represented in red). Nuclei were visualized by DAPI staining (blue).

FluoTag-X2 anti-GFAP AF647

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-GFAP coupled to Alexa Fluor 647 (Cat. No. N3802-AF647, dilution 1:500). A Greyscale image of the staining performed with the anti-GFAP single-domain antibody N3802-AF647. B Greyscale image of the counterstaining performed with an anti-VGlut1 single-domain antibody N1602-At488. C DAPI signal of the depicted section. D Merge of AC. False color representation of A in magenta, B in green and C in blue.

FluoTag-X2 anti-GFAP AF568

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-GFAP coupled to AZDye 568 (Cat. No. N3802-AF568, dilution 1:500). A Greyscale image of the staining performed with the anti-GFAP single-domain antibody N3802-AF568. B Greyscale image of the counterstaining performed with an anti-VGlut1 single-domain antibody N1602-At488. C DAPI signal of the depicted section. D Merge of AC. False color representation of A in red, B in green and C in blue.

FluoTag-X2 anti-GFAP At488

PFA-fixed hippocampal primary neuronal cultures from rats were stained with FluoTag®-X2 anti-GFAP coupled to Atto 488 (Cat. No. N3802-At488, dilution 1:500). A Greyscale image of the staining performed with the anti-GFAP single-domain antibody N3802-At488. B Greyscale image of the counterstaining performed with an anti-PSD95 single-domain antibody N3702-AF647. C DAPI signal of the depicted section. D Merge of AC. False color representation of A in green, B in magenta and C in blue.

FluoTag®-X2 anti-GFAP

Cat No: N3802 Category:

400,00 

FluoTag-X2 anti-GFAP is a fluorescently conjugated single-domain antibody that recognizes mouse and rat GFAP. The single-domain antibody carries two fluorophores and is equipped with specially designed linkers to work in expansion microscopy or enhance post-staining fixation for applications like dSTORM and DNA-PAINT.

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Glial fibrillary acidic protein (GFAP) belongs to type III intermediate filaments (like vimentin) and is expressed in various cell types throughout the human body. However, in the central nervous system, it is almost exclusively present in astrocytes, resulting in an important marker to differentiate neurons from astrocytes. GFAP is used primarily as an excellent astrocyte cellular marker.

FluoTags® can be equipped with a single fluorophore for more quantitative readouts (FluoTag®-Q), with two fluorophores per single-domain antibody (FluoTag®-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores (FluoTag®-X4). For more detailed information on the FluoTags®, please check our Technology Section.

Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μl N3802-At488-L AB_3076117
AZDye568 200 μl N3802-AF568-L AB_3076115
Atto643 200 μl N3802-At643-L AB_3076118
Alexa647 200 μl N3802-AF647-L AB_3076116
AbberiorStar635P 200 μl N3802-Ab635P-L AB_3076114
Related Products: -
Clone: 1D12
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: GFAP
Specificity: Recognizes murine GFAP; other species or isoforms not yet tested.
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Look at detailed protocols in our Resource Section.

 

References:
  1. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives. Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (ICC/IF; tested species: rat)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.