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Immunostaining of PFA-fixed mouse hippocampus neurons with FluoTag®-X2 AZDye568 anti-PSD95 (Cat. No. N3702-AF568, dilution 1:500, represented in red) and FluoTag®-X2 Atto488 anti-GFAP (Cat. No. N3802-At488, dilution 1:500, represented in cyan). Nuclei were visualized by DAPI staining (gray). Figure modified from Kilisch et al., 2023 (see References below).

Confocal and STED images of primary hippocampal culture neurons stained using FluoTag®-X2 anti-PSD95 coupled to AberriorStar635P (Cat. No. N3702-Ab635P, dilution 1:500; displayed in hot red). Counterstaining performed with the FluoTag®-X2 anti-Syt1 (Cat. No. N2302-AF568, dilution 1:500; pre-synaptic marker displayed in hot cyan) and FluoTag®-X2 anti-GFAP (Cat. No. N3802-At488, dilution 1:500; glia marker displayed in gray). Four selected regions denoted by white squares are magnified, displaying conventional lateral PSD shapes (asterisks) and the perforated synapses (arrowheads) only recognizable under STED microscopy. Figure modified from Kilisch et al., 2023 (see References below).

IHC in mouse cerebellar cortex fixed in 4% formaldehyde for 24h and cut into 50 μm thick sections using a vibratome. Brightfield fluorescence imaging overview and the respective zoom-in (bottom) on the granular cell layer (G) and molecular layer (M). FluoTag®-X2 anti-PSD95 AbberiorStar635P (Cat. No. N3702-Ab635P, dilution 1:500; displayed in red) was used with recombinant anti-Synaptotagmin 1 antibody (Synaptic Systems) to reveal pre-synapses (cyan). DAPI staining in gray. FluoTag®-X2 anti-PSD95 gives a bright signal in the Purkinje cell layer at the axosomatic synapses, directly contacting the cell body of Purkinje cells (arrowheads). Figure modified from Kilisch et al., 2023 (see References below).

FluoTag®-X2 anti-PSD95

Cat No: N3702 Category:

400,00 €

A fluorophore-conjugated single-domain antibody that specifically recognizes mouse and rat PSD95. FluoTag®-X2 anti-PSD95 enables direct and highly specific fluorescent detection of PSD95 without the need for secondary antibodies.

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FluoTag®-X2 anti-PSD95 is derived from an in-house developed single-domain antibody (sdAb) that specifically recognizes a PSD95 (Postsynaptic Density Protein 95). PSD95 (alternative names: PSD-95, SAP-90 and DLG4) is a member of the MAGUK family, and it plays an essential role in forming a dynamic scaffold at the post-synapses. It functions as an organizing centrum of receptors, ion channels, kinases, and other relevant players in achieving synaptic plasticity and molecular changes during long-term potentiation.

Our nanobody binds selectively and strongly to one of the PDZ domains of PSD95, and it does not bind to either of its close family members, PSD93 or SAP102. Its characterization was published in the Int J Mol Sci. 2023.

FluoTag®-X2 anti-PSD95 is directly conjugated to two fluorophores per sdAb (FluoTag®-X2 variant). To learn more about the FluoTags®, please visit our Technology section here.

MSDS
Protocols
Reconstitution and Storage
IF (ICC) Protocol
Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μl N3702-At488-L AB_3076105
AZDye568 200 μl N3702-AF568-L AB_3076103
Atto643 200 μl N3702-At643-L AB_3076106
Alexa647 200 μl N3702-AF647-L AB_2936216
AbberiorStar635P 200 μl N3702-Ab635P-L AB_3076102
Related Products: -
Clone: 1B2
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: 1:500 (corresponding to 5 nM final concentration) , 5 µg/mL (IHC)
Capacity: N/A
Antigen: PSD95
Targets: PSD95
Specificity: Recognizes mouse and rat PSD95 (other species not tested). It does not cross-react with PSD93, SAP97 or SAP102.
Formulation:

The single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives. Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (ICC/IF, IHC; tested species: rat, mouse)
  2. Akter Y, Jones G, Daskivich GJ, Shifflett V, Vargas KJ, Hruska M. Combining nanobody labeling with STED microscopy reveals input-specific and layer-specific organization of neocortical synapses. PLoS Biol. Published online April 4, 2025. doi:10.1371/journal.pbio.3002649 (ICC/IF, IHC; STED; mouse brain cryosections, rat primary cortical neuron cultures, transfected HEK293T)
  3. Macarrón-Palacios V, Hubrich J, do Rego Barros Fernandes Lima MA, Metzendorf NG, Kneilmann S, Trapp M, Acuna C, Patrizi A, D’Este E, Kilimann MW. Paralemmin-1 controls the nanoarchitecture of the neuronal submembrane cytoskeleton. Sci Adv. 2025 Mar 7;11(10):eadt3724. doi: 10.1126/sciadv.adt3724. Epub 2025 Mar 7. PMID: 40053592. (ICC/IF, STED; rat hippocampal primary neurons)
  4. Marcassa G, Dascenco D, Lorente-Echeverría B, Daaboul D, Vandensteen J, Leysen E, Baltussen L, Howden AJM, de Wit J. Synaptic signatures and disease vulnerabilities of layer 5 pyramidal neurons. Nat Commun. 2025 Jan 2;16(1):228. doi: 10.1038/s41467-024-55470-w. PMID: 39747884; PMCID: PMC11697078 (IHC; mouse)
  5. Mougios N, Cotroneo ER, Imse N, et al. NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals. Nat Commun. 2024;15(1):8771. Published 2024 Oct 10. doi:10.1038/s41467-024-53030-w
  6. Shaib AH, Chouaib AA, Chowdhury R, et al. One-step nanoscale expansion microscopy reveals individual protein shapes. Nat Biotechnol. Published online October 9, 2024. doi:10.1038/s41587-024-02431-9 (ICC/IF; tested species: rat; hippocampal cultured neurons)
  7. Mougios N, Opazo F, Rizzoli SO, Reshetniak S. Trafficking proteins show limited differences in mobility across different postsynaptic spines. iScience. 2023;26(2):105971. Published 2023 Jan 13. doi:10.1016/j.isci.2023.105971 (ICC/IF; tested species: rat)
  8. Queiroz Zetune Villa Real K, Mougios N, Rehm R, et al. A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy. Small Methods. 2023;7(10):e2300218. doi:10.1002/smtd.202300218 (ICC/IF; tested species: rat)
  9. Paquette AF, Carbone BE, Vogel S, et al. The human milk component myo-inositol promotes neuronal connectivity. Proc Natl Acad Sci U S A. 2023;120(30):e2221413120. doi:10.1073/pnas.2221413120 (ICC/IF; tested species: rat)
  10. Wang L, Pang K, Zhou L, et al. A cross-species proteomic map reveals neoteny of human synapse development. Nature. 2023;622(7981):112-119. doi:10.1038/s41586-023-06542-2 (ICC/IF, IHC; tested species: human, mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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