Immunostaining of PFA-fixed mouse hippocampus neurons with FluoTag®-X2 AZDye568 anti-PSD95 (Cat. No. N3702-AF568, dilution 1:500, represented in red) and FluoTag®-X2 Atto488 anti-GFAP (Cat. No. N3802-At488, dilution 1:500, represented in cyan). Nuclei were visualized by DAPI staining (gray). Figure modified from Kilisch et al., 2023 (see References below).

Confocal and STED images of primary hippocampal culture neurons stained using FluoTag®-X2 anti-PSD95 coupled to AberriorStar635P (Cat. No. N3702-Ab635P, dilution 1:500; displayed in hot red). Counterstaining performed with the FluoTag®-X2 anti-Syt1 (Cat. No. N2302-AF568, dilution 1:500; pre-synaptic marker displayed in hot cyan) and FluoTag®-X2 anti-GFAP (Cat. No. N3802-At488, dilution 1:500; glia marker displayed in gray). Four selected regions denoted by white squares are magnified, displaying conventional lateral PSD shapes (asterisks) and the perforated synapses (arrowheads) only recognizable under STED microscopy. Figure modified from Kilisch et al., 2023 (see References below).

IHC in mouse cerebellar cortex fixed in 4% formaldehyde for 24h and cut into 50 μm thick sections using a vibratome. Brightfield fluorescence imaging overview and the respective zoom-in (bottom) on the granular cell layer (G) and molecular layer (M). FluoTag®-X2 anti-PSD95 AbberiorStar635P (Cat. No. N3702-Ab635P, dilution 1:500; displayed in red) was used with recombinant anti-Synaptotagmin 1 antibody (Synaptic Systems) to reveal pre-synapses (cyan). DAPI staining in gray. FluoTag®-X2 anti-PSD95 gives a bright signal in the Purkinje cell layer at the axosomatic synapses, directly contacting the cell body of Purkinje cells (arrowheads). Figure modified from Kilisch et al., 2023 (see References below).

FluoTag®-X2 anti-PSD95

Name: FluoTag®-X2 anti-PSD95
SKU: N3702
Availability: InStock

Cat No: N3702 Category: Primary sdAbs

400,00 €

A fluorophore-conjugated single-domain antibody that specifically recognizes mouse and rat PSD95. FluoTag®-X2 anti-PSD95 enables direct and highly specific fluorescent detection of PSD95 without the need for secondary antibodies.

Clear

FluoTag®-X2 anti-PSD95 is derived from an in-house developed single-domain antibody (sdAb) that specifically recognizes a PSD95 (Postsynaptic Density Protein 95). PSD95 (alternative names: PSD-95, SAP-90 and DLG4) is a member of the MAGUK family, and it plays an essential role in forming a dynamic scaffold at the post-synapses. It functions as an organizing centrum of receptors, ion channels, kinases, and other relevant players in achieving synaptic plasticity and molecular changes during long-term potentiation.

Our nanobody binds selectively and strongly to PDZ domain 2 of PSD95, and it does not bind to either of its close family members, PSD93, SAP97 or SAP102. Its characterization was published in the Int J Mol Sci. 2023.

FluoTag®-X2 anti-PSD95 is directly conjugated to two fluorophores per sdAb (FluoTag®-X2 variant). To learn more about the FluoTags®, please visit our Technology section here.

MSDS

Variations:

Conjugation	Amount	Cat No.	RRID
Atto488	200 μL	N3702-At488-L	AB_3076105
AZDye568	200 μL	N3702-AF568-L	AB_3076103
Atto643	200 μL	N3702-At643-L	AB_3076106
Alexa647	200 μL	N3702-AF647-L	AB_2936216
abberior STAR 635P	200 μL	N3702-Ab635P-L	AB_3076102

FluoTag®-X2 anti-PSD95

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