Immunostaining of PFA-fixed mouse hippocampus neurons with FluoTag®-X2 AZDye568 anti-PSD95 (Cat. No. N3702-AF568, dilution 1:500, represented in red) and FluoTag®-X2 Atto488 anti-GFAP (Cat. No. N3802-At488, dilution 1:500, represented in cyan). Nuclei were visualized by DAPI staining (gray). Figure modified from Kilisch et al., 2023 (see References below).

Confocal and STED images of primary hippocampal culture neurons stained using FluoTag®-X2 anti-PSD95 coupled to AberriorStar635P (Cat. No. N3702-Ab635P, dilution 1:500; displayed in hot red). Counterstaining performed with the FluoTag®-X2 anti-Syt1 (Cat. No. N2302-AF568, dilution 1:500; pre-synaptic marker displayed in hot cyan) and FluoTag®-X2 anti-GFAP (Cat. No. N3802-At488, dilution 1:500; glia marker displayed in gray). Four selected regions denoted by white squares are magnified, displaying conventional lateral PSD shapes (asterisks) and the perforated synapses (arrowheads) only recognizable under STED microscopy. Figure modified from Kilisch et al., 2023 (see References below).

IHC in mouse cerebellar cortex fixed in 4% formaldehyde for 24h and cut into 50 μm thick sections using a vibratome. Brightfield fluorescence imaging overview and the respective zoom-in (bottom) on the granular cell layer (G) and molecular layer (M). FluoTag®-X2 anti-PSD95 AbberiorStar635P (Cat. No. N3702-Ab635P, dilution 1:500; displayed in red) was used with recombinant anti-Synaptotagmin 1 antibody (Synaptic Systems) to reveal pre-synapses (cyan). DAPI staining in gray. FluoTag®-X2 anti-PSD95 gives a bright signal in the Purkinje cell layer at the axosomatic synapses, directly contacting the cell body of Purkinje cells (arrowheads). Figure modified from Kilisch et al., 2023 (see References below).

FluoTag®-X2 anti-PSD95

Cat No: N3702 Category:


FluoTag-X2 anti-PSD95 is a fluorescently conjugated single-domain antibody that recognizes mouse and rat PSD95.

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PSD95 (alternative names: PSD-95 or SAP-90) is a member of the MAGUK family, and it plays an essential role in forming a dynamic scaffold at the post-synapses. It functions as an organizing centrum of receptors, ion channels, kinases, and other relevant players in achieving synaptic plasticity and molecular changes during long-term potentiation.

Our nanobody binds selectively and strongly to one of the PDZ domains of PSD95, and it does not bind to either of its close family members, PSD93 or SAP102.

FluoTags are directly conjugated to fluorophores; however, they can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores, decorating the target protein with 4 fluorophores in total (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

Conjugation Amount Cat No. RRID
Atto488 200 μl N3702-At488-L AB_3076105
AZDye568 200 μl N3702-AF568-L AB_3076103
Atto643 200 μl N3702-At643-L AB_3076106
Alexa647 200 μl N3702-AF647-L AB_2936216
AbberiorStar635P 200 μl N3702-Ab635P-L AB_3076102
Related Products: -
Clone: 1B2
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: 1:500 (corresponding to 5 nM final concentration) , 5 µg/mL (IHC),
Capacity: N/A
Antigen: -
Targets: PSD95
Specificity: Recognizes mouse and rat PSD95 (other species not tested). It does not cross-react with PSD93, SAP97 or SAP102
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

You find suggested protocols on our Resources page

  1. Kilisch M, Gere-Becker M, Wüstefeld L, et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives. Int J Mol Sci. 2023;24(8):7294. Published 2023 Apr 14. doi:10.3390/ijms24087294 (ICC/IF, IHC; tested species: rat, mouse)
  2. Mougios N, Opazo F, Rizzoli SO, Reshetniak S. Trafficking proteins show limited differences in mobility across different postsynaptic spines. iScience. 2023;26(2):105971. Published 2023 Jan 13. doi:10.1016/j.isci.2023.105971 (ICC/IF; tested species: rat)
  3. Queiroz Zetune Villa Real K, Mougios N, Rehm R, et al. A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy. Small Methods. 2023;7(10):e2300218. doi:10.1002/smtd.202300218 (ICC/IF; tested species: rat)
  4. Paquette AF, Carbone BE, Vogel S, et al. The human milk component myo-inositol promotes neuronal connectivity. Proc Natl Acad Sci U S A. 2023;120(30):e2221413120. doi:10.1073/pnas.2221413120 (ICC/IF; tested species: rat)
  5. Wang L, Pang K, Zhou L, et al. A cross-species proteomic map reveals neoteny of human synapse development. Nature. 2023;622(7981):112-119. doi:10.1038/s41586-023-06542-2 (ICC/IF, IHC; tested species: human, mouse)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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