Immunostaining of PFA-fixed mouse hippocampus neurons with FluoTag®-X2 AZDye568 anti-PSD95 (Cat. No. N3702-AF568, dilution 1:500, represented in red) and FluoTag®-X2 Atto488 anti-GFAP (Cat. No. N3802-At488, dilution 1:500, represented in cyan). Nuclei were visualized by DAPI staining (gray). Figure modified from Kilisch et al., 2023 (see References below).

Confocal and STED images of primary hippocampal culture neurons stained using FluoTag®-X2 anti-PSD95 coupled to AberriorStar635P (Cat. No. N3702-Ab635P, dilution 1:500; displayed in hot red). Counterstaining performed with the FluoTag®-X2 anti-Syt1 (Cat. No. N2302-AF568, dilution 1:500; pre-synaptic marker displayed in hot cyan) and FluoTag®-X2 anti-GFAP (Cat. No. N3802-At488, dilution 1:500; glia marker displayed in gray). Four selected regions denoted by white squares are magnified, displaying conventional lateral PSD shapes (asterisks) and the perforated synapses (arrowheads) only recognizable under STED microscopy. Figure modified from Kilisch et al., 2023 (see References below).

IHC in mouse cerebellar cortex fixed in 4% formaldehyde for 24h and cut into 50 μm thick sections using a vibratome. Brightfield fluorescence imaging overview and the respective zoom-in (bottom) on the granular cell layer (G) and molecular layer (M). FluoTag®-X2 anti-PSD95 AbberiorStar635P (Cat. No. N3702-Ab635P, dilution 1:500; displayed in red) was used with recombinant anti-Synaptotagmin 1 antibody (Synaptic Systems) to reveal pre-synapses (cyan). DAPI staining in gray. FluoTag®-X2 anti-PSD95 gives a bright signal in the Purkinje cell layer at the axosomatic synapses, directly contacting the cell body of Purkinje cells (arrowheads). Figure modified from Kilisch et al., 2023 (see References below).