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FluoTag-X2 anti-mScarlet-i At488

PFA-fixed Cos7 cells expressing a TOM70-nfmScarlet fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mScarlet-i coupled to Atto 488 (Cat. No. N1302-At488, dilution 1:500). A Greyscale image of the staining performed with N1302-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.

sdAb anti-mScarlet-i

Cat No: N1305 Category:

275,00 € – 625,00 €

sdAb anti-mScarlet-i is an in-house developed single-domain antibody (sdAb) recognizing mScarlet variants:-i, H and 3 with high affinity and specificity.

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mScarlet was created in vitro using a synthetic consensus template from various red fluorescent proteins (ref). It is one of the brightest (~3x brighter than mCherry) and truly monomeric fluorescent proteins, making it ideal for cellular imaging (FPbase).  mScarlet can generally be employed in two distinct versions. Each version carries a distinct single amino acid substitution, mScarlet-I (T74I) which features an accelerated maturation speed, and mScarlet-H (M164H) which displays higher photostability. An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”.

Our nanobody binds specifically and strongly to many mScarlet variants (validated for mScarlet-I, mScarlet-H and mScarlet-3). Have a look at our FP Specificity Chart.

 

MSDS
MSDS: sdAb unconjugated C-terminal Cys
MSDS: sdAb-HRP
Protocols
Reconstitution and Storage
Maleimide labeling
Variations:
Conjugation Amount Cat No. RRID
HRP 200 μl N1305-HRP -
unconjugated C-terminal Cys 250 μg N1305-250ug -
Biotin 250 μg N1305-Biotin -
DBCO 250 μg N1305-DBCO -
Related Products:

FluoTag®-X2 anti-mScarlet-i (Cat. No. N1302)

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Clone: 2B12
Host: Alpaca
Produced in: E.coli
Application:

IF

Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly.
Dilution: N/A
Capacity: N/A
Antigen: mScarlet-i
Targets: mScarlet-i, mCherry
Specificity: Recognizes mScarlet-i and mScarlet-3 in its native conformation. Cross-reacts with some mRFP-derived red fluorescent proteins like mCherry.
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C.

The HRP-conjugated sdAbs are lyophilized from HRP protector buffer and should be reconstituted with 200 µL pure deionized water for storage at 4°C or with 200 µL high purity, sterile 50% glycerol for storage at -80°C.

For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage.
Protocols:

For more details, click the Protocols button above or check our FP Specificity Chart.
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.