FluoTag®-X2 anti-mScarlet is derived from an in-house developed single-domain antibody (sdAb) recognizing mScarlet-i with high affinity and specificity.
mScarlet was created in vitro using a consensus synthetic template from various red fluorescent proteins (ref). It is one of the brightest (~3x brighter than mCherry) and truly monomeric fluorescent proteins, making it ideal for cellular imaging (FPbase). mScarlet can generally be employed in two distinct versions. Each version carries a distinct a single amino acid substitution, mScarlet-I (T74I) which features an accelerated maturation speed and mScarlet-H (M164H) which displays higher photostability.
Our nanobody binds specifically and strongly to all three mScarlet variants. Have a look at our specificity chart in the resource section.
For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.
|Specificity:||Recognizes mScarlet-i in its native conformation. Cross-reacts with some mRFP-derived red fluorescent proteins like mCherry.|
|Formulation:||Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.|
|Storing:||Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.|
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|