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PFA fixed COS cells transfected with mScarlet-i-tubulin (A) stained with FluoTag-X2 anti-mScarlet-i Atto488 (B). Overlay in (C)

FluoTag®-X2 anti-mScarlet-i

Cat No: N1302 Category:

375,00 €

FluoTag®-X2 anti-mScarlet is derived from an in-house developed single-domain antibody (sdAb) recognizing mScarlet-i with high affinity and specificity.

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mScarlet was created in vitro using a synthetic consensus template from various red fluorescent proteins (ref). It is one of the brightest (~3x brighter than mCherry) and truly monomeric fluorescent proteins, making it ideal for cellular imaging (FPbase).  mScarlet can generally be employed in two distinct versions. Each version carries a distinct single amino acid substitution, mScarlet-I (T74I) which features an accelerated maturation speed, and mScarlet-H (M164H) which displays higher photostability. An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”

Our nanobody binds specifically and strongly to all three mScarlet variants. Have a look at our specificity chart in our Resource Section.

FluoTags are directly conjugated to fluorophores, however, they can be equipped with a single fluorophore for more quantitative readouts (FluoTag-Q), with two fluorophores per single-domain antibody (FluoTag-X2), and we also developed a blend of two sdAbs bindings simultaneously the target proteins and each bearing two fluorophores, decorating the target protein with 4 fluorophores in total (FluoTag-X4). For more detailed information on the FluoTags, please check our Technology Section.

MSDS Protocols
Variations:
Amount Conjugation Cat No.
Atto488 200 μl N1302-At488-L
AZDye568 200 μl N1302-AF568-L
Atto643 200 μl N1302-At643-L
Alexa647 200 μl N1302-AF647-L
AbberiorStar635P 200 μl N1302-Ab635P-L
Related Products: -
Clone: 2B12
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: mScarlet-i
Specificity: Recognizes mScarlet-i in its native conformation. Cross-reacts with some mRFP-derived red fluorescent proteins like mCherry.
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

Western Blotting is not recommended. The sdAbs tend to recognize native protein conformation only.

Look at detailed protocols and our specificity chart in our Resource Section.

 

 
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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