PFA fixed COS cells transfected with mScarlet-i-tubulin (A) stained with FluoTag-X2 anti-mScarlet-i Atto488 (B). Overlay in (C).

Immunofluorescence analysis of KOLF2.1J human iPSCs transfected with pmScarlet3_C1 plasmid (Addgene #189753). Post-fixation, cells were stained with FluoTag®-X2 anti-mScarlet-i coupled to Atto 488 (Cat. No. N1302-At488, dilution 1:500). A The N1302-At488 specific signal is shown in green. B The mScarlet3 fluorescence is shown in red. C Nuclei were visualized by DAPI staining. D Merge of A – C confirms that FluoTag®-X2 anti-mScarlet-i specifically recognizes the mScarlet3 variant. The images were captured under 40x magnification. Image courtesy of Victoria Menne, King’s College London.

PFA-fixed Cos7 cells expressing a TOM70-nfmScarlet fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mScarlet-i coupled to Alexa Fluor 647 (Cat. No. N1302-AF647, dilution 1:500). A Greyscale image of the staining performed with N1302-AF647. B False color representation of the image shown in A is displayed in magenta (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in magenta and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfmScarlet fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mScarlet-i coupled to AZDye 568 (Cat. No. N1302-AF568, dilution 1:500). A Greyscale image of the staining performed with N1302-AF568. B False color representation of the image shown in A is displayed in red (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in red and C in blue.

PFA-fixed Cos7 cells expressing a TOM70-nfmScarlet fusion protein (nf: non-fluorescent) were stained with FluoTag®-X2 anti-mScarlet-i coupled to Atto 488 (Cat. No. N1302-At488, dilution 1:500). A Greyscale image of the staining performed with N1302-At488. B False color representation of the image shown in A is displayed in green (coloring according to the excitation wavelength of the employed fluorophore). C The corresponding DAPI signal of the depicted section. D Merge of A and C. False color representation of A in green and C in blue.