FluoTag®-X2 anti-mScarlet-i

Cat No: N1302 Category:


FluoTag®-X2 anti-mScarlet is derived from an in-house developed single-domain antibody (sdAb) recognizing mScarlet-i with high affinity and specificity.

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mScarlet was created in vitro using a consensus synthetic template from various red fluorescent proteins (ref). It is one of the brightest (~3x brighter than mCherry) and truly monomeric fluorescent proteins, making it ideal for cellular imaging (FPbase).  mScarlet can generally be employed in two distinct versions. Each version carries a distinct a single amino acid substitution, mScarlet-I (T74I) which features an accelerated maturation speed and mScarlet-H (M164H) which displays higher photostability.

Our nanobody binds specifically and strongly to all three mScarlet variants. Have a look at our specificity chart in the resource section.

For detailed information regarding our FluoTag-Q, -X2 and -X4 series check our technology section here.

Conjugation Amount Cat No.
AbberiorStar580 200 μl N1302-Ab580-L
AbberiorStar635P 200 μl N1302-Ab635P-L
Alexa647 200 μl N1302-AF647-L
Atto488 200 μl N1302-At488-L
AZDye568 200 μl N1302-AF568-L
Related Products: -
Clone: 2B12
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500
Capacity: N/A
Antigen: mScarlet-i
Specificity: Recognizes mScarlet-i in its native conformation. Cross-reacts with some mRFP-derived red fluorescent proteins like mCherry.
Formulation: Lyophilized from PBS pH 7.4. Reconstitute with 200 µL 50 % glycerol including 0.1 % sodium azid as preservative if applicable.
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Specificity Chart

References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.