400,00 €
FluoTag®-X2 anti-mScarlet is derived from our sdAb anti-RFP (clone 2B12) and binds specifically and strongly to many mScarlet variants (validated for mScarlet-I, mScarlet-H and mScarlet-3). It also recognizes DsRed, mRFP, mCherry and others DsRed family members. Conjugated to fluorophores, it enables direct and highly specific fluorescent detection, eliminating the need for secondary antibodies.
mScarlet was created in vitro using a synthetic consensus template from various red fluorescent proteins (ref). It is one of the brightest (~3x brighter than mCherry) and truly monomeric fluorescent proteins, making it ideal for cellular imaging (FPbase). mScarlet can generally be employed in two distinct versions. Each version carries a distinct single amino acid substitution, mScarlet-I (T74I) which features an accelerated maturation speed, and mScarlet-H (M164H) which displays higher photostability. An open-source database with the most currently available variants can be found in the fluorescence protein database “fpbase”.
FluoTag®-X2 anti-mScarlet-i is derived from our sdAb anti-RFP (clone 2B12) and binds specifically and strongly to many mScarlet variants (validated for mScarlet-I, mScarlet-H and mScarlet-3). It also recognizes DsRed, mRFP, mCherry and others DsRed family members. For a detailed overview, refer to our FP Specificity Chart.
FluoTag®-X2 anti-mScarlet-i is directly conjugated to two fluorophores per sdAb (FluoTag®–X2 variant). To learn more about the FluoTags®, please visit our Technology section here.
Variations: |
|
||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Related Products: | |||||||||||||||||||||||||
Clone: | 2B12 | ||||||||||||||||||||||||
Host: | Alpaca | ||||||||||||||||||||||||
Produced in: | E.coli | ||||||||||||||||||||||||
Application: |
IF (ICC) Note: This product is not recommended for detecting proteins in Western blot, as sdAbs tend to recognize native/folded proteins mainly. |
||||||||||||||||||||||||
Dilution: | 1:500 (corresponding to 5 nM final concentration) | ||||||||||||||||||||||||
Capacity: | N/A | ||||||||||||||||||||||||
Antigen: | mCherry | ||||||||||||||||||||||||
Targets: | mRFP, mScarlet-i, mCherry | ||||||||||||||||||||||||
Specificity: | Recognizes mScarlet-i, mScarlet-H, mScarlet-3 in its native conformation. Cross-reacts with some mRFP-derived variants like mCherry, mOrange2, DsRed1, DsRED2 and tdTomato. | ||||||||||||||||||||||||
Formulation: |
A single sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA (US-Origin). For more details, click the Protocols button above and check Reconstitution and Storage. |
||||||||||||||||||||||||
kDa: | - | ||||||||||||||||||||||||
Ext Coef: | - | ||||||||||||||||||||||||
Shipping: | Ambient temperature | ||||||||||||||||||||||||
Storage: |
Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the Protocols button above and check Reconstitution and Storage. |
||||||||||||||||||||||||
Protocols: |
Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page. |
||||||||||||||||||||||||
References: |
|
||||||||||||||||||||||||
Notice: | To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious. | ||||||||||||||||||||||||
Legal terms: | By purchasing this product you agree to our general terms and conditions. |