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Multiplexing image of indirect immostainging of PFA fixed rat hippocampus neurons with mouse anti-Synaptoporin (SYSY 102 011) (green) and mouse anti-Tau (SYSY 314 011) (red). FluoTag-X2 anti-Mouse IgG2a/b was used to decorate both primary mouse monoclonals. Nuclei were visualized using DAPI.

sdAb anti-Mouse IgG2a/b

Cat No: N2705 Category:

250,00 € – 600,00 €

sdAb anti-Mouse IgG2a/b binds to mouse-IgG2a and IgG2b subtype specifically and with high affinity.

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Most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).

Our anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.

MSDS Protocols
Variations:
Amount Conjugation Cat No.
unconjugated C-terminal Cys 250 μg N2705-250µg
Biotin 250 μg N2705-Biotin
DBCO 250 μg N2705-DBCO
HRP 200 μl N2705-HRP
Related Products: -
Clone: 14A4
Host: Alpaca
Produced in: E.coli
Application: IF, IHC, WB
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: Mouse IgG2
Specificity: Recognizes native Mouse IgG2a and IgG2b. It does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Formulation: DBCO and biotin-conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. HRP-conjugated sdAbs are lyophilized from HRP-Protector. Reconstitute with 250 µL pure water (unconjugated sdAbs) or with 250 µL 50% glycerol for storage at -80°C (DBCO-, Biotin-conjugated sdAbs). Use unconjugated sdAbs immediately after reconstitution. Reconstitute HRP-conjugated sdAbs with 200 µL pure water.
kDa: 15.4
Ext Coef: 20128
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols:

Multiplexing and IF protocols can be found on our Resources page
References: -
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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