sdAb anti-ALFA Halo fusion

Cat No: N1541 Category:

400,00 

The sdAb anti-ALFA Halo fusion combines a single-domain antibody (sdAb) targeting the ALFA tag with a dehalogenase-based technology. The sdAb anti-ALFA is fused to the Halo protein tag sequence at the C-terminus.

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The sdAb anti-ALFA Halo fusion combines an alpaca-derived single-domain antibody (sdAb) targeting the ALFA tag with a dehalogenase-based technology. The sdAb anti-ALFA is fused to the Halo protein tag sequence at the C-terminus. The final application of the sdAb anti-ALFA Halo fusion depends on the Halo ligand used. For instance, when combined with a fluorescent Halo ligand, our sdAb anti-ALFA Halo fusion can be used in immunofluorescence for detailed protein visualization and manipulation.

The ALFA system is NanoTag’s patented technology, consisting of the ALFA tag—a rationally designed epitope tag (SRLEEELRRRLTE) – and a set of high-affinity anti-ALFA sdAbs, available in various formats to suit diverse application needs. To learn more about the ALFA system, please visit our Technology section here.

Halo refers to a modified bacterial enzyme, haloalkane dehalogenase, engineered to covalently bind synthetic ligands. These ligands can be conjugated to various functional groups such as fluorophores, biotin, or beads, enabling numerous biochemical, proteomic, and imaging applications.

Variations:
Conjugation Amount Cat No. RRID
- 50 µg N1541 -
Related Products: -
Clone: 1G5
Host: Alpaca
Produced in: E.coli
Application: IF, IHC
Dilution: For IF (ICC), use the sdAb anti-ALFA Halo fusion at 1:500 dilution.
Capacity: N/A
Antigen: ALFA-tag
Targets: ALFA-tag
Specificity: Recognizes the ALFA tag (SRLEEELRRRLTE).
Formulation: sdAb anti-ALFA Halo fusion is lyophilized from PBS pH 7.4 with 2% BSA (US-Origin). After reconstitution in 200 μL of sterile 50% glycerol (v/v) in deionized water, the final concentration of sdAb anti-ALFA Halo fusion is 0.25 mg/mL. For more details, navigate to 'Protocols' then 'Reconstitution and Storage'.
kDa: 38.0
Ext Coef: 60118
Shipping: Ambient temperature
Storing: Store lyophilized reagents at 2-8°C for up to 12 months. Post-reconstitution, store at -80°C. For details, navigate to 'Protocols' then 'Reconstitution and Storage'.
Protocols:

To find information about the reconstitution and storage of sdAb Halo fusions, go to ‘Protocols’ and select ‘Reconstitution and Storage’.

References:
  1. Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. PMID: 31562305; PMCID: PMC6764986.
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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