GST Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.
GST Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees high accessibility of the sdAbs and largely eliminates batch-to-batch variations.
Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GST Selector thus features high affinity and superior capacity for GST fusion proteins while showing negligible non-specific background.
GST Selector is compatible with conventional physiological buffers and with more stringent compositions. As guidance, please look at some suggestions present in our IP protocol. GST Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
|Capacity:||> 2.5 μg GST per μl of packed beads|
|Specificity:||Recognizes Schistosoma japonicum Glutathione S-transferase (GST).|
|Formulation:||50 % slurry in PBS containing 20 % Ethanol|
|Storing:||Store at 4°C for up to 12 months. Do not freeze!|
Suggested protocols for IP and elutions using our Selector system can be found here: IP protocol
This and other protocols can be found on our Resources page.
|Notice:||To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.|
|Legal terms:||By purchasing this product you agree to our general terms and conditions.|