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Immunostaining of PFA fixed rat hippocampus neurons with FluoTag®-X2 anti-Synaptotagmin-1 AF568 (Cat. No. N4302-AZ568-L, dilution 1:500). The confocal image is shown on the left side and the corresponding STED image is shown on the right side.

FluoTag®-X2 anti-Synaptotagmin 1 (A51)

Cat No: N4302 Category:

400,00 €

FluoTag®-X2 anti-Synaptotagmin 1 A51 (SYT1) is an alpaca single-domain antibody (sdAb) that recognizes specifically the cytoplasmic domain of murine Synaptotagmin 1 with high affinity and specificity.

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Synaptotagmin 1 (Syt1) belongs to a family with 17 members. Synaptotagmins are all single-pass transmembrane proteins containing two domains binding to calcium at their intracellular portion.  Synaptotagmin 1 is enriched at the pre-synaptic boutons on axon terminals, associated with synaptic vesicles, and is implicated in triggering vesicle fusion and neurotransmitter release upon a local increase of calcium concentration.

Our nanobody recognizes strongly and specifically the cytosolic portion of Syt1.

 

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
Atto488 200 μl N4302-At488-L AB_3076138
AZDye568 200 μl N4302-AF568-L AB_3076136
Atto643 200 μl N4302-At643-L AB_3076139
Alexa647 200 μl N4302-AF647-L AB_3076137
AbberiorStar635P 200 μl N4302-Ab635P-L AB_3076135
Related Products: -
Clone: A51
Host: Alpaca
Produced in: E.coli
Application: IF
Dilution: 1:500 (corresponding to 5 nM final concentration)
Capacity: N/A
Antigen: -
Targets: Synaptotagmin 1 (cytosolic)
Specificity: Recognizes the cytosolic domain of rat and mouse Synaptotagmin 1 (other species not tested).
Formulation: The single sdAb clone was lyophilized from PBS pH 7.4, containing 2% BSA (US-Origin). Reconstitute with 200 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 200 µl, the concentration of single-domain antibody is 2.5 µM
kDa: -
Ext Coef: -
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Protocols:

This Product is not recommended to be used to detect proteins in Western Blott, sdAbs tend to recognize native/folded protein mainly.

Look at detailed protocols in our Resource Section.
References:
  1. Queiroz Zetune Villa Real K, Mougios N, Rehm R, et al. A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy. Small Methods. 2023;7(10):e2300218. doi:10.1002/smtd.202300218
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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