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Direct immunohistochemistry of a rat hippocampus region. A VGLUT1 positive nerve-terminals were visualized with FluoTag®-X2 anti-VGLUT1 AbberiorSTAR® 580 (Cat. No. N1602; dilution 1:500; false color representation in green). B Indirect immunohistochemistry counterstaining with a mouse monoclonal anti-VGLUT1 (SySy Cat. Co. 135 011). VGLUT1 positiv terminals were revealed with a Cy5 conjugate seconday antibody (false color representation in red). C Overlay of both channels.

sdAb anti-VGLUT1

Cat No: N1605 Category:

Price range: 290,00 € through 655,00 €

sdAb anti-VGLUT1 is derived from a single-domain antibody (sdAb) recognizing specifically VGLUT1 with high affinity and specificity.

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sdAb anti-VGLUT1 is a single-domain antibody (sdAb) that specifically recognizes a vesicular glutamate transporter 1 (VGLUT1) protein. VGLUT1 is primarily located on the synaptic vesicles of glutamatergic synapses. Its function is to fill the vesicles with the neurotransmitter glutamate before they fuse to the plasma membrane and release their content from the pre-synaptic compartment into the synaptic cleft to stimulate postsynaptic receptors. VGLUT1 harnesses the energy from the proton gradient, which is generated by the vesicular ATPase, to transport glutamate into the vesicle.

Our sdAb binds selectively and strongly to a cytoplasmic loop formed by amino acids 258-302 of the rat and mouse VGLUT1.

MSDS
Protocols
Reconstitution and Storage
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N1605-250ug AB_3076007
Biotin 250 μg N1605-Biotin AB_3076008
DBCO 250 μg N1605-DBCO AB_3076009
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FluoTag®X2 anti-VGLUT1 (Cat. No. N1602)

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Clone: Nb9
Host: Llama immune library
Produced in: E.coli
Application: IF, IHC, IP
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: vGlut1
Specificity: Recognizes a cytosolic loop formed by amino acids 258-302 of rat and mouse VGLUT1. Based on the high sequence similarity within this loop, sdAb anti-VGLUT1 is expected to also recognize human VGLUT1 (not experimentally validated).
Formulation:

DBCO- and Biotin-conjugated sdAbs (1:1 labeling on the C-terminus) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyophilized from 5 mM KPi pH 6.0, 300 mM NaCl, 0.5 mM EDTA pH 6.0. Reconstitute with 250 µL pure deionized water for immediate use or with 250 µL sterile 50% glycerol for storage at -80°C. For more details, click the Protocols button above and check Reconstitution and Storage.
kDa: 16.2
Ext Coef: 32618
Shipping: Ambient temperature
Storage:

Vials containing lyophilized reagent can be stored at 2-8°C for up to 12 months. After reconstitution, store at -80°C for up to 6 months. Working aliquots can be stored at -20°C for up to 4 weeks. For more details, click the click the Protocols button above and check Reconstitution and Storage.
Protocols:

Relevant protocols can be found under the Protocols button above. For additional information, visit our Resources page.
References:
  1. Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO. Heat denaturation enables multicolor X10-STED microscopy. Sci Rep. 2023;13(1):5366. Published 2023 Apr 1. doi:10.1038/s41598-023-32524-5 (ICC/IF; primary neurons and synaptic vesicles from rat)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
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