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Direct immunohistochemistry of a rat hippocampus region (1:500). (A) VGLUT1 positive nerve-terminals were visualized with FluoTag-X2 anti-VGLUT1 AbberiorSTAR® 580 (false color representation in green). (B) Indirect immunohistochemistry counterstaining with a mouse monoclonal anti-VGLUT1 (SySy cat. no. 135 011). VGLUT1 positiv terminals were revealed with a Cy5 conjugate seconday antibody (false color representation in red). (C) Overlay of both channels.

sdAb anti-VGlut1

Cat No: N1605 Category:

275,00 € – 625,00 €

sdAb anti-VGLUT1 is derived from a single-domain antibody (sdAb) recognizing specifically VGLUT1 with high affinity and specificity.

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vGlut1 is primarily located on the synaptic vesicles of glutamatergic synapses. Its function is to fill the vesicles with the neurotransmitter glutamate before they fuse to the plasma membrane and release their content from the pre-synaptic compartment into the synaptic cleft to stimulate the postsynaptic receptors. vGlut1 uses the energy from the proton gradient generated by the vesicular ATPase to transport glutamate inside the vesicle.

Our sdAb binds selectively and firmly to a cytoplasmic portion of the vGlut1.

MSDS Protocols
Variations:
Conjugation Amount Cat No. RRID
unconjugated C-terminal Cys 250 μg N1605-250ug AB_3076007
Biotin 250 μg N1605-Biotin AB_3076008
DBCO 250 μg N1605-DBCO AB_3076009
Related Products: -
Clone: Nb9
Host: Synthetic Library
Produced in: E.coli
Application: IHC, IP
Dilution: N/A
Capacity: N/A
Antigen: -
Targets: vGlut1
Specificity: Recognizes the cytosolic domain of rat VGLUT1 (other species not tested).
Formulation: DBCO and biotin conjugated sdAbs (1:1 labeling on the c-term.) are lyophilized from PBS pH 7.4. Unconjugated sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA. Reconsitute with 250 µL pure water for immidate use or with 250 µL 50 % glycerol for storage at -80 °C.
kDa: 16.2
Ext Coef: 32618
Shipping: Ambient temperature
Storing: Vials containing lyophilized protein can be stored at 4°C for upto 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
Protocols: -
References:
  1. Saal KA, Shaib AH, Mougios N, Crzan D, Opazo F, Rizzoli SO. Heat denaturation enables multicolor X10-STED microscopy. Sci Rep. 2023;13(1):5366. Published 2023 Apr 1. doi:10.1038/s41598-023-32524-5 (ICC/IF; primary neurons and synaptic vesicles from rat)
Notice: To be used in vitro/ for research only. Non-toxic, non-hazardous, non-infectious.
Legal terms: By purchasing this product you agree to our general terms and conditions.

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